LOCUS       Exported               10366 bp ds-DNA     circular SYN 20-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..15
                     /locus_tag="pUC19 AatII 15bp"
                     /label=pUC19 AatII 15bp
     LTR             42..282
                     /locus_tag="Minimal 3'TR"
                     /label=Minimal 3'TR
     repeat_region   42..58
                     /locus_tag="PB Terminal Repeat"
                     /label=PB Terminal Repeat
     misc_feature    42..45
                     /locus_tag="TTAA"
                     /label=TTAA
     repeat_region   90..108
                     /locus_tag="PB Inverted Repeat"
                     /label=PB Inverted Repeat
     promoter        578..1767
                     /locus_tag="hEF1a promoter(1)"
                     /label=hEF1a promoter
                     /label=hEF1a promoter(1)
                     /note="/vntifkey=29"
     misc_signal     806..814
                     /locus_tag="SD"
                     /label=SD
     misc_feature    1363..1381
                     /locus_tag="EF1aPro-fwd-2"
                     /label=EF1aPro-fwd-2
     protein_bind    1502..1508
                     /locus_tag="AP1 binding"
                     /label=AP1 binding
     misc_feature    1626..1645
                     /locus_tag="EF1aPro-fwd"
                     /label=EF1aPro-fwd
     misc_signal     1749..1752
                     /locus_tag="SA"
                     /label=SA
     promoter        1768
                     /locus_tag="hEF1a promoter"
                     /label=hEF1a promoter
                     /note="/vntifkey=29"
     misc_feature    1769..1770
                     /locus_tag="mCMV promoter"
                     /label=mCMV promoter
     misc_recomb     1782..1793
                     /locus_tag="AttR1"
                     /label=AttR1
     misc_feature    1787..1793
                     /locus_tag="O"
                     /label=O
     misc_feature    1794..1806
                     /locus_tag="attL1"
                     /label=attL1
     misc_feature    complement(1807..1816)
                     /locus_tag="attL2"
                     /label=attL2
     misc_feature    1857..1862
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1878..1898
                     /locus_tag="SV40_NLS"
                     /label=SV40_NLS
     misc_feature    1899..1904
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    1905..2417
                     /locus_tag="Cse2"
                     /label=Cse2
     misc_feature    2418..2423
                     /locus_tag="SpeI"
                     /label=SpeI
     misc_feature    2424..2444
                     /locus_tag="SV40_NLS(4)"
                     /label=SV40_NLS
                     /label=SV40_NLS(4)
     misc_feature    2445..2510
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    2514..2534
                     /locus_tag="SV40_NLS(2)"
                     /label=SV40_NLS
                     /label=SV40_NLS(2)
     misc_feature    2535..2540
                     /locus_tag="MluI"
                     /label=MluI
     misc_feature    2541..3170
                     /locus_tag="Cas6"
                     /label=Cas6
     misc_feature    3171..3176
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3177..3197
                     /locus_tag="SV40_NLS(1)"
                     /label=SV40_NLS
                     /label=SV40_NLS(1)
     misc_feature    3198..3260
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    3264..3284
                     /locus_tag="SV40_NLS(3)"
                     /label=SV40_NLS
                     /label=SV40_NLS(3)
     misc_feature    3285..3290
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    3291..5987
                     /locus_tag="Cas3"
                     /label=Cas3
     misc_feature    5988..5993
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    5994..6014
                     /locus_tag="SV40_NLS(5)"
                     /label=SV40_NLS
                     /label=SV40_NLS(5)
     misc_feature    6018..6025
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    6044..6058
                     /locus_tag="attR2 site"
                     /label=attR2 site
     misc_feature    complement(6044..6050)
                     /locus_tag="O'"
                     /label=O'
     misc_feature    6092..6217
                     /locus_tag="EMCV IRES"
                     /label=EMCV IRES
     misc_feature    6218..6688
                     /locus_tag="EMCV IRES(1)"
                     /label=EMCV IRES
                     /label=EMCV IRES(1)
     misc_feature    complement(6224..6243)
                     /locus_tag="IRES-rev"
                     /label=IRES-rev
     CDS             6686..7396
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    7428..7968
                     /locus_tag="Rabbit hemoglobin beta poly A"
                     /label=Rabbit hemoglobin beta poly A
     LTR             8062..8371
                     /locus_tag="Minimal 5'TR"
                     /label=Minimal 5'TR
     repeat_region   8333..8351
                     /locus_tag="PB Inverted Repeat(1)"
                     /label=PB Inverted Repeat
                     /label=PB Inverted Repeat(1)
     repeat_region   8355..8371
                     /locus_tag="PB Terminal Repeat(1)"
                     /label=PB Terminal Repeat
                     /label=PB Terminal Repeat(1)
     misc_feature    8368..8371
                     /locus_tag="TTAA(1)"
                     /label=TTAA
                     /label=TTAA(1)
     misc_feature    8395..8409
                     /locus_tag="pUC19 PvuII 15bp"
                     /label=pUC19 PvuII 15bp
     rep_origin      complement(8572..9245)
                     /direction=LEFT
                     /locus_tag="pUC ori"
                     /label=pUC ori
     rep_origin      complement(8613..9295)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      complement(8631..9219)
                     /direction=LEFT
                     /locus_tag="pMB1 origin of replication"
                     /label=pMB1 origin of replication
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(8631..9183)
                     /locus_tag="RNAII transcript"
                     /label=RNAII transcript
                     /note="RNAII transcript (complementary strand)"
     -35_signal      9037..9042
                     /locus_tag="RNAI promoter (-35;TTGAAG)"
                     /label=RNAI promoter (-35;TTGAAG)
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9059..9064
                     /locus_tag="RNAI promoter (-10;GCTACA)"
                     /label=RNAI promoter (-10;GCTACA)
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9073..9180
                     /locus_tag="RNAI transcript"
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(9193..9198)
                     /locus_tag="RNAII promoter (-10;CGTAAT)"
                     /label=RNAII promoter (-10;CGTAAT)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9214..9219)
                     /locus_tag="RNAII promoter (-35;TTGAGA)"
                     /label=RNAII promoter (-35;TTGAGA)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(9390..10250)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="beta-lactamase (AmpR)"
                     /product="beta-lactamase"
                     /label=beta-lactamase (AmpR)
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9390..10250)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_recomb     complement(join(9525..9528,9530..9535))
                     /locus_tag="BsaI"
                     /label=BsaI
     -10_signal      complement(10294..10299)
                     /locus_tag="bla promoter (-10); GAGACA"
                     /label=bla promoter (-10); GAGACA
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(10315..10320)
                     /locus_tag="bla promoter (-35); TTCAAA"
                     /label=bla promoter (-35); TTCAAA
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt
      901 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      961 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     1021 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     1081 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     1141 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     1201 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     1261 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     1321 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     1381 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     1441 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     1501 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     1561 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1621 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1681 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1741 ttccatttca ggtgtcgtga gaattagcgg tggcggccat cacaagtttg tacaaaaaag
     1801 caggctaccc agctttgctg gcgccggaac caattcagtc gactggatcc ggtaccgaat
     1861 tcgccgccac catggcccca aagaagaaga gaaaggtgag atctgaagcc gatgagatcg
     1921 acgcaatggc actgtacagg gcatggcagc agctggacaa cggatcttgc gcacagatca
     1981 ggcgcgtgag cgagcctgat gagctgaggg acatcccagc cttctatcgg ctggtgcagc
     2041 cctttggctg ggagaatcct agacaccagc aggccctgct gaggatggtg ttttgtctga
     2101 gcgccggcaa gaacgtgatc cggcaccagg acaagaagag cgagcagacc acaggaatct
     2161 ccctgggacg cgccctggcc aattccggcc ggatcaacga gcggagaatc ttccagctga
     2221 tcagggccga tcgcacagcc gacatggtgc agctgaggcg cctgctgacc cacgcagagc
     2281 ctgtgctgga ttggccactg atggcccgca tgctgacatg gtggggcaag cgggagagac
     2341 agcagctgct ggaggacttc gtgctgacca caaataagaa cgccaagcgg actgctgatg
     2401 gcagtgaatt tgagtccact agtccaaaga agaagagaaa ggtgggaagc ggagcaacca
     2461 acttcagcct gctgaagcag gccggcgatg tggaggagaa tccaggcccc atgccaaaga
     2521 agaagagaaa ggtgacgcgt gaatacctga gcaaagtgat catcgcaagg gcatggtcca
     2581 gggacctgta tcagctgcac cagggcctgt ggcacctgtt ccctaataga ccagatgccg
     2641 ccagggactt cctgtttcac gtggagaaga ggaacacacc cgagggctgt cacgtgctgc
     2701 tgcagtccgc ccagatgccc gtgagcaccg cagtggccac agtgatcaag accaagcagg
     2761 tggagttcca gctgcaagtg ggcgtgccac tgtactttag gctgcgcgcc aatcccatca
     2821 agaccatcct ggataaccag aagcgcctgg actctaaggg caatatcaag cggtgcagag
     2881 tgcctctgat caaggaggcc gagcagatcg cctggctgca gagaaagctg ggcaacgccg
     2941 ccagggtgga ggatgtgcac cctatcagcg agcggccaca gtatttcagc ggcgacggca
     3001 agtccggcaa gatccagacc gtgtgctttg agggcgtgct gaccatcaac gatgccccag
     3061 ccctgatcga cctggtgcag cagggaatcg gacctgctaa gtcaatggga tgtgggctgc
     3121 tgtcactggc acctctgaag cggactgctg atggcagtga atttgagtcc tctagaccaa
     3181 agaagaagag aaaggtgggc tccggcgagg gcaggggaag tcttctaaca tgcggggacg
     3241 tggaggaaaa tcccggccca atgccaaaga agaagagaaa ggtgggatcc gaggagccct
     3301 tcaagtacat ctgccactat tggggcaaga gctccaagag cctgacaaag ggcaacgata
     3361 tccacctgct gatctaccac tgcctggacg tggcagcagt ggcagattgt tggtgggacc
     3421 agtccgtggt gctgcagaat accttttgtc ggaacgagat gctgtctaag cagagagtga
     3481 aggcctggct gctgttcttt atcgccctgc acgatatcgg caagttcgac atccggtttc
     3541 agtataagtc cgccgagtct tggctgaagc tgaatccagc cacaccctct ctgaacggcc
     3601 ctagcaccca gatgtgcaga aagttcaatc acggcgccgc cggcctgtac tggtttaacc
     3661 aggactctct gagcgagcag agcctgggcg atttcttttc cttctttgac gcagcacctc
     3721 acccctacga gtcctggttc ccttgggtgg aggcagtgac cggacaccac ggctttatcc
     3781 tgcattctca ggaccaggat aagagccggt gggagatgcc agccagcctg gcctcctacg
     3841 ccgcacagga taagcaggcc agagaggagt ggatcagcgt gctggaggcc ctgttcctga
     3901 ccccagcagg actgtccatc aatgatatcc cacctgactg ctctagcctg ctggccggct
     3961 tttgttccct ggccgactgg ctgggctctt ggaccacaac caatacattc ctgtttaacg
     4021 aggatgcacc aagcgacatc aacgccctga ggacctactt ccaggatcgg cagcaggacg
     4081 cctccagagt gctggagctg tccggcctgg tgtctaataa gaggtgctac gagggagtgc
     4141 acgccctgct ggataacggc tatcagccaa ggcagctgca ggtgctggtg gacgccctgc
     4201 ctgtggcacc aggactgaca gtgatcgagg cacctaccgg cagcggcaag acagagaccg
     4261 ccctggccta tgcctggaag ctgatcgatc agcagatcgc cgactccgtg atcttcgccc
     4321 tgccaacaca ggccaccgcc aatgcaatgc tgacccggat ggaggcctcc gcctctcacc
     4381 tgttttcctc tcccaacctg atcctggccc acggcaacag caggttcaat cacctgtttc
     4441 agtctatcaa gagccgcgcc atcacagagc agggacagga ggaggcctgg gtgcagtgct
     4501 gtcagtggct gagccagtcc aataagaagg tgttcctggg ccagatcggc gtgtgcacca
     4561 tcgatcaggt gctgatctct gtgctgcctg tgaagcacag gtttatcagg ggactgggaa
     4621 tcggcagaag cgtgctgatc gtggatgagg tgcacgccta cgacacatat atgaacggcc
     4681 tgctggaggc cgtgctgaag gcacaggcag acgtgggcgg ctccgtgatc ctgctgtctg
     4741 ccaccctgcc aatgaagcag aagcagaagc tgctggatac atacggcctg cacaccgacc
     4801 ccgtggagaa caattccgcc tatcctctga tcaattggag gggagtgaac ggagcacagc
     4861 ggttcgacct gctggcccac cctgagcagc tgccaccaag gttttctatc cagcccgagc
     4921 ctatctgcct ggccgatatg ctgccagacc tgaccatgct ggagaggatg atcgctgccg
     4981 ccaatgcagg agcacaggtg tgcctgatct gtaacctggt ggatgtggcc caggtgtgct
     5041 accagaggct gaaggagctg aacaatacac aggtggacat cgatctgttc cacgcccggt
     5101 ttaccctgaa tgaccggaga gagaaggaga acagagtgat ctctaacttc ggcaagaatg
     5161 gcaagagaaa cgtgggcaga atcctggtgg ccacacaggt ggtggagcag agcctggacg
     5221 tggatttcga ctggctgatc acccagcact gccccgccga tctgctgttt cagcggctgg
     5281 gcagactgca caggcaccac cgcaagtatc ggcctgccgg ctttgagatc ccagtggcca
     5341 caatcctgct gcctgacgga gagggatacg gcaggcacga gcacatctat agcaatgtgc
     5401 gcgtgatgtg gcggacccag cagcacatcg aggagctgaa cggcgcctct ctgttctttc
     5461 cagatgccta ccgccagtgg ctggacagca tctatgacga tgccgagatg gatgagcccg
     5521 agtgggtggg caatggcatg gacaagttcg agtccgccga gtgtgagaag cggttcaagg
     5581 ccaggaaggt gctgcagtgg gccgaggagt actccctgca ggataacgac gagacaatcc
     5641 tggccgtgac cagagatggc gagatgtctc tgcccctgct gccttatgtg cagacaagct
     5701 ccggcaagca gctgctggat ggccaggtgt acgaggacct gagccacgag cagcagtatg
     5761 aggccctggc cctgaacagg gtgaatgtgc ccttcacctg gaagcggagc ttcagcgaag
     5821 tggtggatga ggacggcctg ctgtggctgg agggcaagca gaatctggat ggctgggtgt
     5881 ggcagggcaa ctccatcgtg atcacataca ccggcgacga gggcatgacc agagtgatcc
     5941 cagccaaccc caagaagaga accgccgacg gcagcgagtt cgagtccgct agcccaaaga
     6001 agaagagaaa ggtgtaagcg gccgcactcg agatatctag acccttgtac aaagtggtga
     6061 tctagctaaa acgcggcctc gaatcgaatt ccgccccccc ccctctccct cccccccccc
     6121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     6541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6601 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6661 acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata acatggccat
     6721 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     6781 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     6841 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     6901 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     6961 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     7021 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     7081 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     7141 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     7201 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     7261 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     7321 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     7381 cgagctgtac aagtaaagcg ctctaggctt aagtatacct cgagttaaat tcactcctca
     7441 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     7501 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     7561 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     7621 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     7681 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     7741 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     7801 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     7861 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     7921 cccagtcata gctgtccctc ttctcttatg gagatccctc gacttaatta accaacaagc
     7981 tcgtcatcgc tttgcagaag agcagagagg atatgctcat cgtctaaaga actacccatt
     8041 ttattatata ttagtcacga tatctataac aagaaaatat atatataata agttatcacg
     8101 taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa aagtcacgta
     8161 aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat cagtgacact
     8221 taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat gtcctaaatg
     8281 cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa tgcatgcgtc
     8341 aattttacgc agactatctt tctagggtta atacgtataa tacatatgat tcagctgcat
     8401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     9541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     9601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     9661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     9721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     9781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     9841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccc
//
