LOCUS       Exported                9820 bp ds-DNA     circular SYN 08-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..15
                     /locus_tag="pUC19 AatII 15bp"
                     /label=pUC19 AatII 15bp
     LTR             42..282
                     /locus_tag="Minimal 3'TR"
                     /label=Minimal 3'TR
     repeat_region   42..58
                     /locus_tag="PB Terminal Repeat"
                     /label=PB Terminal Repeat
     misc_feature    42..45
                     /locus_tag="TTAA"
                     /label=TTAA
     repeat_region   90..108
                     /locus_tag="PB Inverted Repeat"
                     /label=PB Inverted Repeat
     promoter        578..1767
                     /locus_tag="hEF1a promoter(1)"
                     /label=hEF1a promoter
                     /label=hEF1a promoter(1)
                     /note="/vntifkey=29"
     misc_signal     806..814
                     /locus_tag="SD"
                     /label=SD
     misc_feature    1363..1381
                     /locus_tag="EF1aPro-fwd-2"
                     /label=EF1aPro-fwd-2
     protein_bind    1502..1508
                     /locus_tag="AP1 binding"
                     /label=AP1 binding
     misc_feature    1626..1645
                     /locus_tag="EF1aPro-fwd"
                     /label=EF1aPro-fwd
     misc_signal     1749..1752
                     /locus_tag="SA"
                     /label=SA
     promoter        1768
                     /locus_tag="hEF1a promoter"
                     /label=hEF1a promoter
                     /note="/vntifkey=29"
     misc_feature    1769..1770
                     /locus_tag="mCMV promoter"
                     /label=mCMV promoter
     misc_recomb     1782..1793
                     /locus_tag="AttR1"
                     /label=AttR1
     misc_feature    1787..1793
                     /locus_tag="O"
                     /label=O
     misc_feature    1794..1806
                     /locus_tag="attL1"
                     /label=attL1
     misc_feature    complement(1807..1816)
                     /locus_tag="attL2"
                     /label=attL2
     misc_feature    1857..1862
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1878..1898
                     /locus_tag="SV40_NLS(4)"
                     /label=SV40_NLS
                     /label=SV40_NLS(4)
     misc_feature    1899..1904
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    1905..3026
                     /locus_tag="Cas7"
                     /label=Cas7
     misc_feature    3027..3032
                     /locus_tag="SpeI"
                     /label=SpeI
     misc_feature    3033..3053
                     /locus_tag="SV40_NLS"
                     /label=SV40_NLS
     misc_feature    3054..3119
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    3123..3143
                     /locus_tag="SV40_NLS(2)"
                     /label=SV40_NLS
                     /label=SV40_NLS(2)
     misc_feature    3144..3149
                     /locus_tag="MluI"
                     /label=MluI
     CDS             3150..3854
                     /locus_tag="Cas5"
                     /label=Cas5
     misc_feature    3855..3860
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3861..3881
                     /locus_tag="SV40_NLS(5)"
                     /label=SV40_NLS
                     /label=SV40_NLS(5)
     misc_feature    3882..3944
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    3948..3968
                     /locus_tag="SV40_NLS(1)"
                     /label=SV40_NLS
                     /label=SV40_NLS(1)
     misc_feature    3969..3974
                     /locus_tag="BamHI"
                     /label=BamHI
     CDS             3975..5477
                     /locus_tag="Cse1"
                     /label=Cse1
     misc_feature    3998
                     /locus_tag="C->T"
                     /label=C->T
     misc_feature    5478..5483
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    5484..5504
                     /locus_tag="SV40_NLS(3)"
                     /label=SV40_NLS
                     /label=SV40_NLS(3)
     misc_feature    5508..5515
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    5534..5548
                     /locus_tag="attR2 site"
                     /label=attR2 site
     misc_feature    complement(5534..5540)
                     /locus_tag="O'"
                     /label=O'
     misc_feature    join(5577..5583,5590..5813)
                     /locus_tag="IRES(2)"
                     /label=IRES
                     /label=IRES(2)
     misc_feature    complement(5714..5733)
                     /locus_tag="IRES-rev"
                     /label=IRES-rev
     misc_feature    5814..5819
                     /locus_tag="IRES"
                     /label=IRES
                     /note="/vntifkey=21"
     misc_feature    join(5820..6105,6107..6165)
                     /locus_tag="IRES(1)"
                     /label=IRES
                     /label=IRES(1)
                     /note="/vntifkey=21"
     CDS             join(6177..6227,6229..6776)
                     /locus_tag="PuroR"
                     /label=PuroR
                     /note="/vntifkey=21"
     misc_feature    6882..7422
                     /locus_tag="Rabbit hemoglobin beta poly A"
                     /label=Rabbit hemoglobin beta poly A
     LTR             7516..7825
                     /locus_tag="Minimal 5'TR"
                     /label=Minimal 5'TR
     repeat_region   7787..7805
                     /locus_tag="PB Inverted Repeat(1)"
                     /label=PB Inverted Repeat
                     /label=PB Inverted Repeat(1)
     repeat_region   7809..7825
                     /locus_tag="PB Terminal Repeat(1)"
                     /label=PB Terminal Repeat
                     /label=PB Terminal Repeat(1)
     misc_feature    7822..7825
                     /locus_tag="TTAA(1)"
                     /label=TTAA
                     /label=TTAA(1)
     misc_feature    7849..7863
                     /locus_tag="pUC19 PvuII 15bp"
                     /label=pUC19 PvuII 15bp
     rep_origin      complement(8026..8699)
                     /direction=LEFT
                     /locus_tag="pUC ori"
                     /label=pUC ori
     rep_origin      complement(8067..8749)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      complement(8085..8673)
                     /direction=LEFT
                     /locus_tag="pMB1 origin of replication"
                     /label=pMB1 origin of replication
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(8085..8637)
                     /locus_tag="RNAII transcript"
                     /label=RNAII transcript
                     /note="RNAII transcript (complementary strand)"
     -35_signal      8491..8496
                     /locus_tag="RNAI promoter (-35;TTGAAG)"
                     /label=RNAI promoter (-35;TTGAAG)
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      8513..8518
                     /locus_tag="RNAI promoter (-10;GCTACA)"
                     /label=RNAI promoter (-10;GCTACA)
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        8527..8634
                     /locus_tag="RNAI transcript"
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(8647..8652)
                     /locus_tag="RNAII promoter (-10;CGTAAT)"
                     /label=RNAII promoter (-10;CGTAAT)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(8668..8673)
                     /locus_tag="RNAII promoter (-35;TTGAGA)"
                     /label=RNAII promoter (-35;TTGAGA)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(8844..9704)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="beta-lactamase (AmpR)"
                     /product="beta-lactamase"
                     /label=beta-lactamase (AmpR)
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(8844..9704)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_recomb     complement(join(8979..8982,8984..8989))
                     /locus_tag="BsaI"
                     /label=BsaI
     -10_signal      complement(9748..9753)
                     /locus_tag="bla promoter (-10); GAGACA"
                     /label=bla promoter (-10); GAGACA
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(9769..9774)
                     /locus_tag="bla promoter (-35); TTCAAA"
                     /label=bla promoter (-35); TTCAAA
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt
      901 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      961 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     1021 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     1081 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     1141 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     1201 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     1261 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     1321 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     1381 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     1441 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     1501 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     1561 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1621 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1681 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1741 ttccatttca ggtgtcgtga gaattagcgg tggcggccat cacaagtttg tacaaaaaag
     1801 caggctaccc agctttgctg gcgccggaac caattcagtc gactggatcc ggtaccgaat
     1861 tcgccgccac catggcccca aagaagaaga gaaaggtgag atctgaatcc aatttcatca
     1921 acatccacgt gctgatctcc cactctccaa gctgcctgaa tagagacgat atgaacatgc
     1981 agaaggacgc catctttggc ggcaagcgga gagtgaggat ctctagccag tccctgaagc
     2041 gggccatgag aaagtctggc tactatgccc agaatatcgg cgagtcctct ctgaggacca
     2101 tccacctggc acagctgagg gacgtgctga gacagaagct gggcgagcgg tttgatcaga
     2161 agatcatcga caagacactg gccctgctga gcggcaagtc cgtggatgag gccgagaaga
     2221 tcagcgccga cgcagtgacc ccatgggtgg tgggagagat cgcatggttc tgtgagcagg
     2281 tggccaaggc cgaggccgat aatctggacg ataagaagct gctgaaggtg ctgaaggagg
     2341 atatcgccgc catcagagtg aacctgcagc agggagtgga catcgccctg agcggcagga
     2401 tggccacatc cggcatgatg accgagctgg gcaaggtgga cggagcaatg tccatcgcac
     2461 acgccatcac cacacaccag gtggactctg atatcgactg gttcacagcc gtggacgatc
     2521 tgcaggagca gggaagcgcc cacctgggaa cccaggagtt cagctccggc gtgttttaca
     2581 gatatgccaa tatcaacctg gcacagctgc aggagaacct gggaggagca tccagggagc
     2641 aggccctgga gatcgccaca cacgtggtgc acatgctggc aaccgaggtg ccaggagcaa
     2701 agcagcgcac ctacgccgcc ttcaatcctg ccgatatggt catggtgaac ttttccgaca
     2761 tgccactgtc tatggccaat gccttcgaga aggccgtgaa ggccaaggat ggcttcctgc
     2821 agccttccat ccaggccttt aaccagtact gggaccgcgt ggccaatgga tatggcctga
     2881 acggagctgc cgcccagttt tccctgtctg atgtggaccc tatcacagcc caggtgaagc
     2941 agatgccaac cctggagcag ctgaagagct gggtgcggaa caatggagag gcaaagcgga
     3001 ctgctgatgg cagtgaattt gagtccacta gtccaaagaa gaagagaaag gtgggaagcg
     3061 gagcaaccaa cttcagcctg ctgaagcagg ccggcgatgt ggaggagaat ccaggcccca
     3121 tgccaaagaa gaagagaaag gtgacgcgtg aacgctccta cctgatcctg agactggcag
     3181 gaccaatgca ggcatgggga cagcctacat tcgagggaac caggccaaca ggccgctttc
     3241 ctacccggtc tggactgctg ggactgctgg gagcctgcct gggcatccag agggacgata
     3301 cctctagcct gcaggccctg agcgagtccg tgcagttcgc cgtgcgctgt gatgagctga
     3361 tcctggacga taggcgcgtg tccgtgacag gcctgcggga ttaccacacc gtgctgggcg
     3421 ccagagagga ctataggggc ctgaagtccc acgagaccat ccagacatgg cgcgagtacc
     3481 tgtgcgacgc ctcttttaca gtggccctgt ggctgacccc acacgcaaca atggtcatca
     3541 gcgagctgga gaaggccgtg ctgaagccac ggtacacccc ctatctgggc cggagaagct
     3601 gccctctgac acacccactg ttcctgggca cctgtcaggc ctccgatccc cagaaggccc
     3661 tgctgaacta cgagcctgtg ggcggcgaca tctattctga ggagagcgtg acaggccacc
     3721 acctgaagtt caccgccagg gatgagccaa tgatcacact gccaaggcag tttgcatcca
     3781 gggagtggta tgtgatcaag ggaggaatgg acgtgagcca gaagcggact gctgatggca
     3841 gtgaatttga gtcctctaga ccaaagaaga agagaaaggt gggctccggc gagggcaggg
     3901 gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccaatgcca aagaagaaga
     3961 gaaaggtggg atccaacctg ctgattgaca actggattcc tgtgcgccca cggaacggag
     4021 gaaaagtcca gattattaat ctgcagagcc tgtactgctc ccgggatcag tggagactga
     4081 gcctgcccag agacgatatg gagctggccg ccctggccct gctggtgtgc atcggccaga
     4141 tcatcgcccc tgccaaggac gatgtggagt tcaggcaccg catcatgaac cctctgaccg
     4201 aggatgagtt tcagcagctg atcgccccat ggatcgacat gttctatctg aatcacgccg
     4261 agcacccctt catgcagaca aagggcgtga aggccaacga cgtgaccccc atggagaagc
     4321 tgctggcagg cgtgtccgga gcaacaaatt gcgccttcgt gaaccagcca ggacagggag
     4381 aggccctgtg cggaggctgt accgccatcg ccctgtttaa tcaggcaaac caggcacctg
     4441 gattcggagg aggctttaag tctggactga ggggaggaac cccagtgacc acattcgtga
     4501 gaggcatcga tctgaggagc acagtgctgc tgaatgtgct gaccctgcca cggctgcaga
     4561 agcagtttcc caatgagagc cacacagaga accagcccac ctggatcaag cctatcaagt
     4621 ctaacgagag catccctgcc agctccatcg gcttcgtgag aggcctgttt tggcagccag
     4681 cccacatcga gctgtgcgac cccatcggca tcggcaagtg ttcttgctgt ggccaggaaa
     4741 gcaatctgag gtacaccggc ttcctgaagg agaagttcac ctttacagtg aacggcctgt
     4801 ggccccaccc tcactctcca tgtctggtga cagtgaagaa gggcgaggtg gaggagaagt
     4861 tcctggcctt taccacatcc gccccctctt ggacccagat cagcagagtg gtggtggaca
     4921 agatcatcca gaacgagaat ggcaacagag tggccgccgt ggtgaatcag ttcaggaaca
     4981 tcgccccaca gtctcccctg gagctgatca tgggcggcta caggaacaat caggccagca
     5041 tcctggagcg gagacacgat gtgctgatgt ttaatcaggg ctggcagcag tatggcaatg
     5101 tgatcaacga gatcgtgaca gtgggcctgg gctacaagac cgccctgaga aaggccctgt
     5161 atacattcgc cgagggcttt aagaacaagg acttcaaggg agcaggcgtg agcgtgcacg
     5221 agaccgccga gaggcacttt taccgccagt ccgagctgct gatccccgat gtgctggcca
     5281 atgtgaactt ctcccaggcc gacgaagtga tcgccgatct gagggacaag ctgcaccagc
     5341 tgtgcgagat gctgtttaac cagtctgtgg ccccatacgc ccaccacccc aagctgatca
     5401 gcacactggc cctggcaagg gccaccctgt ataagcacct gagggagctg aagccacagg
     5461 gaggaccttc taatggagct agcccaaaga agaagagaaa ggtgtaagcg gccgcactcg
     5521 agatatctag acccttgtac aaagtggtga tctagctaaa acgcggcctc gaatcgaatt
     5581 ccgccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     5641 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5701 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5761 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5821 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5881 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5941 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6001 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6061 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6121 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga
     6181 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6241 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6301 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6361 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6421 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6481 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6541 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6601 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6661 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6721 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     6781 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgattcgaa
     6841 tgtacagatc tggatcctag gcttaagtat acctcgagtt aaattcactc ctcaggtgca
     6901 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     6961 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     7021 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     7081 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     7141 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     7201 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     7261 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     7321 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     7381 catagctgtc cctcttctct tatggagatc cctcgactta attaaccaac aagctcgtca
     7441 tcgctttgca gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta
     7501 tatattagtc acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     7561 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     7621 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     7681 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     7741 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     7801 acgcagacta tctttctagg gttaatacgt ataatacata tgattcagct gcattaatga
     7861 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7921 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7981 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8281 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8401 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8461 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8521 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8581 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8641 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8701 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8761 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8821 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8881 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8941 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9001 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9061 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc
//
