LOCUS       Exported               10330 bp ds-DNA     circular SYN 20-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 11, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..15
                     /locus_tag="pUC19 AatII 15bp"
                     /label=pUC19 AatII 15bp
     LTR             42..282
                     /locus_tag="Minimal 3'TR"
                     /label=Minimal 3'TR
     repeat_region   42..58
                     /locus_tag="PB Terminal Repeat"
                     /label=PB Terminal Repeat
     misc_feature    42..45
                     /locus_tag="TTAA"
                     /label=TTAA
     repeat_region   90..108
                     /locus_tag="PB Inverted Repeat"
                     /label=PB Inverted Repeat
     promoter        578..1767
                     /locus_tag="hEF1a promoter(1)"
                     /label=hEF1a promoter
                     /label=hEF1a promoter(1)
                     /note="/vntifkey=29"
     misc_signal     806..814
                     /locus_tag="SD"
                     /label=SD
     misc_feature    1363..1381
                     /locus_tag="EF1aPro-fwd-2"
                     /label=EF1aPro-fwd-2
     protein_bind    1502..1508
                     /locus_tag="AP1 binding"
                     /label=AP1 binding
     misc_feature    1626..1645
                     /locus_tag="EF1aPro-fwd"
                     /label=EF1aPro-fwd
     misc_signal     1749..1752
                     /locus_tag="SA"
                     /label=SA
     promoter        1768
                     /locus_tag="hEF1a promoter"
                     /label=hEF1a promoter
                     /note="/vntifkey=29"
     misc_feature    1769..1770
                     /locus_tag="mCMV promoter"
                     /label=mCMV promoter
     misc_recomb     1782..1793
                     /locus_tag="AttR1"
                     /label=AttR1
     misc_feature    1787..1793
                     /locus_tag="O"
                     /label=O
     misc_feature    1794..1806
                     /locus_tag="attL1"
                     /label=attL1
     misc_feature    complement(1807..1816)
                     /locus_tag="attL2"
                     /label=attL2
     misc_feature    1857..1862
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1878..1898
                     /locus_tag="SV40_NLS"
                     /label=SV40_NLS
     misc_feature    1899..1904
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    1905..2417
                     /locus_tag="Cse2"
                     /label=Cse2
     misc_feature    2418..2423
                     /locus_tag="SpeI"
                     /label=SpeI
     misc_feature    2424..2444
                     /locus_tag="SV40_NLS(4)"
                     /label=SV40_NLS
                     /label=SV40_NLS(4)
     misc_feature    2445..2510
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    2514..2534
                     /locus_tag="SV40_NLS(2)"
                     /label=SV40_NLS
                     /label=SV40_NLS(2)
     misc_feature    2535..2540
                     /locus_tag="MluI"
                     /label=MluI
     misc_feature    2541..3170
                     /locus_tag="Cas6"
                     /label=Cas6
     misc_feature    3171..3176
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3177..3197
                     /locus_tag="SV40_NLS(1)"
                     /label=SV40_NLS
                     /label=SV40_NLS(1)
     misc_feature    3198..3260
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    3264..3284
                     /locus_tag="SV40_NLS(3)"
                     /label=SV40_NLS
                     /label=SV40_NLS(3)
     misc_feature    3285..3290
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    3291..5987
                     /locus_tag="Cas3"
                     /label=Cas3
     misc_feature    5988..5993
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    5994..6014
                     /locus_tag="SV40_NLS(5)"
                     /label=SV40_NLS
                     /label=SV40_NLS(5)
     misc_feature    6018..6025
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    6044..6058
                     /locus_tag="attR2 site"
                     /label=attR2 site
     misc_feature    complement(6044..6050)
                     /locus_tag="O'"
                     /label=O'
     misc_feature    join(6087..6093,6100..6323)
                     /locus_tag="IRES(2)"
                     /label=IRES
                     /label=IRES(2)
     misc_feature    complement(6224..6243)
                     /locus_tag="IRES-rev"
                     /label=IRES-rev
     misc_feature    6324..6329
                     /locus_tag="IRES"
                     /label=IRES
                     /note="/vntifkey=21"
     misc_feature    join(6330..6615,6617..6675)
                     /locus_tag="IRES(1)"
                     /label=IRES
                     /label=IRES(1)
                     /note="/vntifkey=21"
     CDS             join(6687..6737,6739..7286)
                     /locus_tag="PuroR"
                     /label=PuroR
                     /note="/vntifkey=21"
     misc_feature    7392..7932
                     /locus_tag="Rabbit hemoglobin beta poly A"
                     /label=Rabbit hemoglobin beta poly A
     LTR             8026..8335
                     /locus_tag="Minimal 5'TR"
                     /label=Minimal 5'TR
     repeat_region   8297..8315
                     /locus_tag="PB Inverted Repeat(1)"
                     /label=PB Inverted Repeat
                     /label=PB Inverted Repeat(1)
     repeat_region   8319..8335
                     /locus_tag="PB Terminal Repeat(1)"
                     /label=PB Terminal Repeat
                     /label=PB Terminal Repeat(1)
     misc_feature    8332..8335
                     /locus_tag="TTAA(1)"
                     /label=TTAA
                     /label=TTAA(1)
     misc_feature    8359..8373
                     /locus_tag="pUC19 PvuII 15bp"
                     /label=pUC19 PvuII 15bp
     rep_origin      complement(8536..9209)
                     /direction=LEFT
                     /locus_tag="pUC ori"
                     /label=pUC ori
     rep_origin      complement(8577..9259)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     rep_origin      complement(8595..9183)
                     /direction=LEFT
                     /locus_tag="pMB1 origin of replication"
                     /label=pMB1 origin of replication
                     /note="pMB1 origin of replication (counter-clockwise) 
                     (RNAII -35 to RNA/DNA switch point)"
     misc_RNA        complement(8595..9147)
                     /locus_tag="RNAII transcript"
                     /label=RNAII transcript
                     /note="RNAII transcript (complementary strand)"
     -35_signal      9001..9006
                     /locus_tag="RNAI promoter (-35;TTGAAG)"
                     /label=RNAI promoter (-35;TTGAAG)
                     /note="RNAI promoter (clockwise); TTGAAG"
     -10_signal      9023..9028
                     /locus_tag="RNAI promoter (-10;GCTACA)"
                     /label=RNAI promoter (-10;GCTACA)
                     /note="RNAI promoter (clockwise); GCTACA"
     misc_RNA        9037..9144
                     /locus_tag="RNAI transcript"
                     /label=RNAI transcript
                     /note="RNAI transcript"
     -10_signal      complement(9157..9162)
                     /locus_tag="RNAII promoter (-10;CGTAAT)"
                     /label=RNAII promoter (-10;CGTAAT)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
     -35_signal      complement(9178..9183)
                     /locus_tag="RNAII promoter (-35;TTGAGA)"
                     /label=RNAII promoter (-35;TTGAGA)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
     CDS             complement(9354..10214)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="beta-lactamase (AmpR)"
                     /product="beta-lactamase"
                     /label=beta-lactamase (AmpR)
                     /note="ampR (confers resistance to ampicillin)"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(9354..10214)
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_recomb     complement(join(9489..9492,9494..9499))
                     /locus_tag="BsaI"
                     /label=BsaI
     -10_signal      complement(10258..10263)
                     /locus_tag="bla promoter (-10); GAGACA"
                     /label=bla promoter (-10); GAGACA
                     /note="bla promoter (counter-clockwise); GAGACA"
     -35_signal      complement(10279..10284)
                     /locus_tag="bla promoter (-35); TTCAAA"
                     /label=bla promoter (-35); TTCAAA
                     /note="bla promoter (counter-clockwise); TTCAAA"
ORIGIN
        1 gaaaagtgcc acctgacgtc atctgttaac attatacgcg tttaacccta gaaagataat
       61 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      121 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      181 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      241 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      301 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
      361 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
      421 cacatatcaa tattatgctc tgctagcgtt taaacccggg cgatcgctct agactagtgc
      481 ggccgctgca gaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
      541 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      601 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      661 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      721 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      781 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      841 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacgcccc tggctgcagt
      901 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      961 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     1021 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     1081 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     1141 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     1201 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     1261 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     1321 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     1381 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     1441 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     1501 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     1561 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     1621 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     1681 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     1741 ttccatttca ggtgtcgtga gaattagcgg tggcggccat cacaagtttg tacaaaaaag
     1801 caggctaccc agctttgctg gcgccggaac caattcagtc gactggatcc ggtaccgaat
     1861 tcgccgccac catggcccca aagaagaaga gaaaggtgag atctgaagcc gatgagatcg
     1921 acgcaatggc actgtacagg gcatggcagc agctggacaa cggatcttgc gcacagatca
     1981 ggcgcgtgag cgagcctgat gagctgaggg acatcccagc cttctatcgg ctggtgcagc
     2041 cctttggctg ggagaatcct agacaccagc aggccctgct gaggatggtg ttttgtctga
     2101 gcgccggcaa gaacgtgatc cggcaccagg acaagaagag cgagcagacc acaggaatct
     2161 ccctgggacg cgccctggcc aattccggcc ggatcaacga gcggagaatc ttccagctga
     2221 tcagggccga tcgcacagcc gacatggtgc agctgaggcg cctgctgacc cacgcagagc
     2281 ctgtgctgga ttggccactg atggcccgca tgctgacatg gtggggcaag cgggagagac
     2341 agcagctgct ggaggacttc gtgctgacca caaataagaa cgccaagcgg actgctgatg
     2401 gcagtgaatt tgagtccact agtccaaaga agaagagaaa ggtgggaagc ggagcaacca
     2461 acttcagcct gctgaagcag gccggcgatg tggaggagaa tccaggcccc atgccaaaga
     2521 agaagagaaa ggtgacgcgt gaatacctga gcaaagtgat catcgcaagg gcatggtcca
     2581 gggacctgta tcagctgcac cagggcctgt ggcacctgtt ccctaataga ccagatgccg
     2641 ccagggactt cctgtttcac gtggagaaga ggaacacacc cgagggctgt cacgtgctgc
     2701 tgcagtccgc ccagatgccc gtgagcaccg cagtggccac agtgatcaag accaagcagg
     2761 tggagttcca gctgcaagtg ggcgtgccac tgtactttag gctgcgcgcc aatcccatca
     2821 agaccatcct ggataaccag aagcgcctgg actctaaggg caatatcaag cggtgcagag
     2881 tgcctctgat caaggaggcc gagcagatcg cctggctgca gagaaagctg ggcaacgccg
     2941 ccagggtgga ggatgtgcac cctatcagcg agcggccaca gtatttcagc ggcgacggca
     3001 agtccggcaa gatccagacc gtgtgctttg agggcgtgct gaccatcaac gatgccccag
     3061 ccctgatcga cctggtgcag cagggaatcg gacctgctaa gtcaatggga tgtgggctgc
     3121 tgtcactggc acctctgaag cggactgctg atggcagtga atttgagtcc tctagaccaa
     3181 agaagaagag aaaggtgggc tccggcgagg gcaggggaag tcttctaaca tgcggggacg
     3241 tggaggaaaa tcccggccca atgccaaaga agaagagaaa ggtgggatcc gaggagccct
     3301 tcaagtacat ctgccactat tggggcaaga gctccaagag cctgacaaag ggcaacgata
     3361 tccacctgct gatctaccac tgcctggacg tggcagcagt ggcagattgt tggtgggacc
     3421 agtccgtggt gctgcagaat accttttgtc ggaacgagat gctgtctaag cagagagtga
     3481 aggcctggct gctgttcttt atcgccctgc acgatatcgg caagttcgac atccggtttc
     3541 agtataagtc cgccgagtct tggctgaagc tgaatccagc cacaccctct ctgaacggcc
     3601 ctagcaccca gatgtgcaga aagttcaatc acggcgccgc cggcctgtac tggtttaacc
     3661 aggactctct gagcgagcag agcctgggcg atttcttttc cttctttgac gcagcacctc
     3721 acccctacga gtcctggttc ccttgggtgg aggcagtgac cggacaccac ggctttatcc
     3781 tgcattctca ggaccaggat aagagccggt gggagatgcc agccagcctg gcctcctacg
     3841 ccgcacagga taagcaggcc agagaggagt ggatcagcgt gctggaggcc ctgttcctga
     3901 ccccagcagg actgtccatc aatgatatcc cacctgactg ctctagcctg ctggccggct
     3961 tttgttccct ggccgactgg ctgggctctt ggaccacaac caatacattc ctgtttaacg
     4021 aggatgcacc aagcgacatc aacgccctga ggacctactt ccaggatcgg cagcaggacg
     4081 cctccagagt gctggagctg tccggcctgg tgtctaataa gaggtgctac gagggagtgc
     4141 acgccctgct ggataacggc tatcagccaa ggcagctgca ggtgctggtg gacgccctgc
     4201 ctgtggcacc aggactgaca gtgatcgagg cacctaccgg cagcggcaag acagagaccg
     4261 ccctggccta tgcctggaag ctgatcgatc agcagatcgc cgactccgtg atcttcgccc
     4321 tgccaacaca ggccaccgcc aatgcaatgc tgacccggat ggaggcctcc gcctctcacc
     4381 tgttttcctc tcccaacctg atcctggccc acggcaacag caggttcaat cacctgtttc
     4441 agtctatcaa gagccgcgcc atcacagagc agggacagga ggaggcctgg gtgcagtgct
     4501 gtcagtggct gagccagtcc aataagaagg tgttcctggg ccagatcggc gtgtgcacca
     4561 tcgatcaggt gctgatctct gtgctgcctg tgaagcacag gtttatcagg ggactgggaa
     4621 tcggcagaag cgtgctgatc gtggatgagg tgcacgccta cgacacatat atgaacggcc
     4681 tgctggaggc cgtgctgaag gcacaggcag acgtgggcgg ctccgtgatc ctgctgtctg
     4741 ccaccctgcc aatgaagcag aagcagaagc tgctggatac atacggcctg cacaccgacc
     4801 ccgtggagaa caattccgcc tatcctctga tcaattggag gggagtgaac ggagcacagc
     4861 ggttcgacct gctggcccac cctgagcagc tgccaccaag gttttctatc cagcccgagc
     4921 ctatctgcct ggccgatatg ctgccagacc tgaccatgct ggagaggatg atcgctgccg
     4981 ccaatgcagg agcacaggtg tgcctgatct gtaacctggt ggatgtggcc caggtgtgct
     5041 accagaggct gaaggagctg aacaatacac aggtggacat cgatctgttc cacgcccggt
     5101 ttaccctgaa tgaccggaga gagaaggaga acagagtgat ctctaacttc ggcaagaatg
     5161 gcaagagaaa cgtgggcaga atcctggtgg ccacacaggt ggtggagcag agcctggacg
     5221 tggatttcga ctggctgatc acccagcact gccccgccga tctgctgttt cagcggctgg
     5281 gcagactgca caggcaccac cgcaagtatc ggcctgccgg ctttgagatc ccagtggcca
     5341 caatcctgct gcctgacgga gagggatacg gcaggcacga gcacatctat agcaatgtgc
     5401 gcgtgatgtg gcggacccag cagcacatcg aggagctgaa cggcgcctct ctgttctttc
     5461 cagatgccta ccgccagtgg ctggacagca tctatgacga tgccgagatg gatgagcccg
     5521 agtgggtggg caatggcatg gacaagttcg agtccgccga gtgtgagaag cggttcaagg
     5581 ccaggaaggt gctgcagtgg gccgaggagt actccctgca ggataacgac gagacaatcc
     5641 tggccgtgac cagagatggc gagatgtctc tgcccctgct gccttatgtg cagacaagct
     5701 ccggcaagca gctgctggat ggccaggtgt acgaggacct gagccacgag cagcagtatg
     5761 aggccctggc cctgaacagg gtgaatgtgc ccttcacctg gaagcggagc ttcagcgaag
     5821 tggtggatga ggacggcctg ctgtggctgg agggcaagca gaatctggat ggctgggtgt
     5881 ggcagggcaa ctccatcgtg atcacataca ccggcgacga gggcatgacc agagtgatcc
     5941 cagccaaccc caagaagaga accgccgacg gcagcgagtt cgagtccgct agcccaaaga
     6001 agaagagaaa ggtgtaagcg gccgcactcg agatatctag acccttgtac aaagtggtga
     6061 tctagctaaa acgcggcctc gaatcgaatt ccgccccccc ccctctccct cccccccccc
     6121 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6181 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6241 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6301 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6361 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6421 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6481 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     6541 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6601 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     6661 aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg cgcctcgcca
     6721 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     6781 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6841 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6901 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6961 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     7021 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     7081 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     7141 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     7201 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     7261 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     7321 ggagcgcacg accccatgca tcgattcgaa tgtacagatc tggatcctag gcttaagtat
     7381 acctcgagtt aaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     7441 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     7501 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     7561 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     7621 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     7681 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     7741 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     7801 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     7861 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     7921 cctcgactta attaaccaac aagctcgtca tcgctttgca gaagagcaga gaggatatgc
     7981 tcatcgtcta aagaactacc cattttatta tatattagtc acgatatcta taacaagaaa
     8041 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
     8101 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
     8161 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
     8221 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
     8281 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatacgt
     8341 ataatacata tgattcagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10321 acatttcccc
//
