LOCUS       pM_416_                 9990 bp ds-DNA     circular     09-SEP-2019
DEFINITION  .
ACCESSION   urn.local...3ec-58sv2hx
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    18..1718
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label=CAG promoter
                     /ApEinfo_fwdcolor=#66ccff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    18..381
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label=CMV Enhancer
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    18..23
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /note="Geneious type: restriction site"
                     /label=SpeI, AhlI, BcuI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    84..371
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /label=CAG_enhancer
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    662..1625
                     /SerialCloner_Color="&hC01400"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label=Chicken beta-actin intron
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1153..1159
                     /Recognition_pattern="GC^TNAGC"
                     /annotation_group="GCTNAGC"
                     /note="Geneious type: restriction site"
                     /label=BlpI, Bpu1102I, Bsp1720I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1310..1317
                     /Recognition_pattern="CG^CCGGCG"
                     /annotation_group="CGCCGGCG"
                     /note="Geneious type: restriction site"
                     /label=MreI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1310..1317
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /note="Geneious type: restriction site"
                     /label=SgrAI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1623..1628
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /note="Geneious type: restriction site"
                     /label=XbaI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(6095..6484,6488..6905)
                     /label=E.coli dam
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1626..1718
                     /SerialCloner_Color="&hA304C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label=Rabbit globin intron
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(6095..6484,6488..6925)
                     /label=Ecoli dam(N132A)
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6906..6925
                     /label=E.coli dam(1)
                     /ApEinfo_label=E.coli dam
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1735..1740
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /note="Geneious type: restriction site"
                     /label=Acc65I, Asp718I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1735..1740
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /note="Geneious type: restriction site"
                     /label=KpnI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1736..1760
                     /created_by="User"
                     /label=Gibson overlap
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1756..1761
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /note="Geneious type: restriction site"
                     /label=AgeI, AsiGI, BshTI, CspAI, PinAI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     motif           1763..1771
                     /SerialCloner_Color="&hB400C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand translation"" \"
                     /SerialCloner_Desc="translation=""AT"" \"
                     /modified_by="User"
                     /label=Kozak sequence
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1772..1772
                     /Original_Bases="ATG"
                     /note="Geneious type: Editing History Deletion"
                     /label=ATG
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1772..5971
                     /SerialCloner_Color="&h0031C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand translation"" \"
                     /SerialCloner_Desc="translation=""MDKKYSIGLDIGTNSVGWAVITDE
                     YKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYL
                     QEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRK
                     KLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEE
                     NPINASGVDAK \"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label=dCas9
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1981..1986
                     /Recognition_pattern="GTA^TAC"
                     /annotation_group="GTATAC"
                     /note="Geneious type: restriction site"
                     /label=BssNAI, Bst1107I, BstZ17I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1986..1991
                     /Recognition_pattern="CACGTC(-3/-3)"
                     /annotation_group="CACGTC"
                     /note="Geneious type: restriction site"
                     /label=BtrI, AjiI, BmgBI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     2201..2209
                     /created_by="User"
                     /label=Loop A
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     2291..2299
                     /created_by="User"
                     /label=Loop B
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     2363..2371
                     /created_by="User"
                     /label=Loop C
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2411..2430)
                     /Sequence="CGTCGGGATTTAGAGAGGCG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /note="Geneious type: primer_bind_reverse"
                     /label=oBK424
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2411..2430
                     /Sequence="CGCCTCTCTAAATCCCGACG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label=oBK423
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2411..2430)
                     /Sequence="CGTCGGGATTTAGAGAGGCG"
                     /Transferred_From="oBK424"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     2453..2461
                     /created_by="User"
                     /label=Loop D
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2483..2489
                     /Recognition_pattern="G^GTNACC"
                     /annotation_group="GGTNACC"
                     /note="Geneious type: restriction site"
                     /label=BstEII, BstPI, Eco91I, EcoO65I, PspEI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2577..2582
                     /Recognition_pattern="C^GTACG"
                     /annotation_group="CGTACG"
                     /note="Geneious type: restriction site"
                     /label=BsiWI, Pfl23II, PspLI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2841..2851)
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /annotation_group="ACNNNNGTAYC"
                     /note="Geneious type: restriction site"
                     /label=BaeI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3327..3332
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /note="Geneious type: restriction site"
                     /label=Bsp1407I, BsrGI, BstAUI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     3365..3373
                     /created_by="User"
                     /label=Loop E
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     3482..3490
                     /created_by="User"
                     /label=Loop F
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3608..3613
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /note="Geneious type: restriction site"
                     /label=EcoRV, Eco32I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3747..3756
                     /Recognition_pattern="GACNN^NNGTC"
                     /annotation_group="GACNNNNGTC"
                     /note="Geneious type: restriction site"
                     /label=PshAI, BoxI, BstPAI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     3782..3790
                     /created_by="User"
                     /label=Loop G
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3851..3856
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /note="Geneious type: restriction site"
                     /label=PvuII
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     4145..4174
                     /created_by="User"
                     /label=Loop H
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4716..4723
                     /Recognition_pattern="RTGC^GCAY"
                     /annotation_group="RTGCGCAY"
                     /note="Geneious type: restriction site"
                     /label=FspAI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     4967..4975
                     /created_by="User"
                     /label=Loop I
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5158..5170)
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /note="Geneious type: restriction site"
                     /label=BarI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     sig_peptide     5222..5230
                     /created_by="User"
                     /label=Loop J
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5393..5412
                     /Sequence="GGCCGAAAACGGATGTTGGC"
                     /Transferred_From="oBK309"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(1)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5403..5426)
                     /Sequence="GCTCTCCGGCGCTAGCCAACATCC"
                     /Hairpin_Tm="64.0"
                     /Tm="69.6"
                     /Self_Dimer_Tm="13.3"
                     /%GC="66.7"
                     /created_by="primer3"
                     /note="Geneious type: primer_bind_reverse"
                     /label=oBK1013
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5411..5416
                     /Recognition_pattern="GCTAG^C"
                     /annotation_group="GCTAGC"
                     /note="Geneious type: restriction site"
                     /label=BmtI, BspOI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5411..5416
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /note="Geneious type: restriction site"
                     /label=NheI, AsuNHI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5441..5453
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /annotation_group="GAACNNNNNNTAC"
                     /note="Geneious type: restriction site"
                     /label=PsrI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5899..5904
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /note="Geneious type: restriction site"
                     /label=XhoI, PaeR7I, Sfr274I, SlaI, StrI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(5904..5927)
                     /Sequence="CACCGTCATGGTCTTTGTAGTCGC"
                     /Transferred_From="oBK591"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(2)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     5904..5927
                     /Sequence="GCGACTACAAAGACCATGACGGTG"
                     /Transferred_From="oBK590"
                     /Transferred_Similarity="100.00%"
                     /label=Binding Region(3)
                     /ApEinfo_label=Binding Region
                     /ApEinfo_fwdcolor=#14c0bd
                     /ApEinfo_revcolor=#4ec02b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     motif           5979..5979
                     /created_by="User"
                     /label=X
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6929..6936
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /note="Geneious type: restriction site"
                     /label=NotI, CciNI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     motif           6952..6952
                     /created_by="User"
                     /label=X(1)
                     /ApEinfo_label=X
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6983..6994
                     /Recognition_pattern="CCANNNNN^NTGG"
                     /annotation_group="CCANNNNNNTGG"
                     /note="Geneious type: restriction site"
                     /label=BstXI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      7102..7603
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""rb_glob_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY \"
                     /label=rb_glob_PA_terminator
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7176..7189
                     /Recognition_pattern="(7/12)GAANNNNNNNTTGG(11/6)"
                     /annotation_group="GAANNNNNNNTTGG"
                     /note="Geneious type: restriction site"
                     /label=AjuI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7211..7221
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /note="Geneious type: restriction site"
                     /label=PflMI, AccB7I, BasI, Van91I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7475..7480
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /note="Geneious type: restriction site"
                     /label=HindIII
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(7645..7663)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""M13_reverse_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7662..7684)
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""M13_pUC_rev_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label=M13_pUC_rev_primer
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(7698..7727)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""lac_promoter"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK \"
                     /label=lac_promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7790..7800
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /note="Geneious type: restriction site"
                     /label=BplI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7792..7804
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /note="Geneious type: restriction site"
                     /label=SfiI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        7833..8012
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_promoter"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS \"
                     /label=SV40_promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7846..7851
                     /Recognition_pattern="C^CTAGG"
                     /annotation_group="CCTAGG"
                     /note="Geneious type: restriction site"
                     /label=AvrII, AspA2I, BlnI, XmaJI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      7868..7945
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_origin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKD \"
                     /label=SV40_origin
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      8026..8157
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label=SV40_PA_terminator
                     /ApEinfo_fwdcolor=#9d1b1c
                     /ApEinfo_revcolor=#9d1b1c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8056..8062
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /annotation_group="GCTCTTC"
                     /note="Geneious type: restriction site"
                     /label=SapI, BspQI, LguI, PciSI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8114..8133
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""EBV_rev_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL \"
                     /label=EBV_rev_primer
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8381..9000)
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""pBR322_origin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS \"
                     /label=pBR322_origin
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9067..9077
                     /Recognition_pattern="GACNNN^NNGTC"
                     /annotation_group="GACNNNNNGTC"
                     /note="Geneious type: restriction site"
                     /label=Eam1105I, AhdI, BmeRI, DriI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(9139..9144)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /note="Geneious type: restriction site"
                     /label=Eco31I, BsaI, Bso31I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(join(9155..9990,1..25))
                     /SerialCloner_Color="&hFF4500"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""Ampicillin"" -
                     gene=""Ampicillin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG \"
                     /label=Ampicillin_Ampicillin
                     /ApEinfo_fwdcolor=#ff797d
                     /ApEinfo_revcolor=#ff102f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9439..9444
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /note="Geneious type: restriction site"
                     /label=PvuI, BpvUI, MvrI, Ple19I
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9550..9555
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /note="Geneious type: restriction site"
                     /label=ScaI, AssI, BmcAI, ZrmI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(9986..9990,1..2)
                     /Recognition_pattern="A^CCWGGT"
                     /annotation_group="ACCWGGT"
                     /note="Geneious type: restriction site"
                     /label=SexAI, CsiI, MabI
                     /ApEinfo_fwdcolor=#7eff74
                     /ApEinfo_revcolor=#7eff74
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(1..95)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /label=AmpR_promoter
                     /ApEinfo_fwdcolor=#346ee0
                     /ApEinfo_revcolor=#346ee0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5879..5899
                     /label=sv40 NLS
                     /ApEinfo_fwdcolor=#fead32
                     /ApEinfo_revcolor=#fead32
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6005..6085
                     /label=3AC3L linker
                     /ApEinfo_fwdcolor=#ffd516
                     /ApEinfo_revcolor=#ffd516
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5906..5971
                     /label=3X FLAG
                     /ApEinfo_fwdcolor=#fe578e
                     /ApEinfo_revcolor=#fe578e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 2291..2689
                     /created_by="User"
                     /label=delta 175-307
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     ORF             5873..5971
                     /created_by="User"
                     /label=NLS-FLAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat ggataaaaag tattctattg gtttagccat
     1801 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa
     1861 atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct
     1921 cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag
     1981 gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc
     2041 caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa
     2101 gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa
     2161 gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct
     2221 gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga
     2281 gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac
     2341 ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat
     2401 tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg
     2461 agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa
     2521 ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta
     2581 cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt
     2641 ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga
     2701 gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga
     2761 cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt
     2821 ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga
     2881 attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt
     2941 aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc
     3001 acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc
     3061 gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta
     3121 tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga
     3181 aacgattact ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt
     3241 catcgagagg atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca
     3301 cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac
     3361 tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct
     3421 gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa
     3481 aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact
     3541 tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga
     3601 gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat
     3661 gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt
     3721 aaagaggcgt cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag
     3781 agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag
     3841 gaactttatg cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc
     3901 acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc
     3961 agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat
     4021 gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca
     4081 gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact
     4141 gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact
     4201 ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa
     4261 ccgtttatct gattacgacg tcgatgccat tgtaccccaa tcctttttga aggacgattc
     4321 aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc
     4381 aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact
     4441 gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact
     4501 tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt
     4561 tgcacagata ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg
     4621 ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca
     4681 attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc
     4741 cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg
     4801 tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa
     4861 ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac
     4921 tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga
     4981 aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca
     5041 agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct
     5101 tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta
     5161 cggtggcttc gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa
     5221 gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg
     5281 ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa
     5341 aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa
     5401 acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa
     5461 atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga
     5521 taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga
     5581 gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt
     5641 aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca
     5701 tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat
     5761 agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc
     5821 catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg acggatcccc
     5881 caagaagaag aggaaagtct cgagcgacta caaagaccat gacggtgatt ataaagatca
     5941 tgacatcgat tacaaggatg acgatgacaa ggctgcagga ggcggtggaa gcgggggaGA
     6001 GCTCtctagt ggcaatagca acgccaactc ccggggcccc agtttttcct ccggactggt
     6061 gcccctgtcc ctgagagggt cacatgCCTG CAGGaagaaa aatcgcgctt ttttgaagtg
     6121 ggcagggggc aagtatcccc tgcttgatga tattaaacgg catttgccca agggcgaatg
     6181 tctggttgag ccttttgtag gtgccgggtc ggtgtttctc aacaccgact tttctcgtta
     6241 tatccttgcc gatatcaata gcgacctgat cagtctctat aacattgtga agatgcgtac
     6301 tgatgagtac gtacaggccg cacgcgagct gtttgttccc gaaacaaatt gcgccgaggt
     6361 ttactatcag ttccgcgaag agttcaacaa aagccaggat ccgttccgtc gggcggtact
     6421 gtttttatat ttgaaccgct acggttacaa cggcctgtgt cgttacaatc tgcgcggtga
     6481 gtttGCCgtg ccgttcggcc gctacaaaaa accctatttc ccggaagcag agttgtatca
     6541 cttcgctgaa aaagcgcaga atgccttttt ctattgtgag tcttacgccg atagcatggc
     6601 gcgcgcagat gatgcatccg tcgtctattg cgatccgcct tatgcaccgc tgtctgcgac
     6661 cgccaacttt acggcgtatc acacaaacag ttttacgctt gaacaacaag cgcatctggc
     6721 ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg ctgatctcca atcacgatac
     6781 gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat gtcgtcaaag ttcgacgcag
     6841 tataagcagc aacggcggca cacgtaaaaa ggtggacgaa ctgctggctt tgtacaaacc
     6901 aggagtcgtt tcacccgcga aaaaataagc ggccgcactc ctcaggtgca ggctgcctat
     6961 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     7021 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     7081 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     7141 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     7201 gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag gtcatcagta
     7261 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     7321 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     7381 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     7441 tcttctctta tgaagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     7501 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7561 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7621 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     7681 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7801 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7861 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7981 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8041 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8101 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8401 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     8461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8641 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9961 cgcgcacatt tccccgaaaa gtgccacctg   
//
