LOCUS       CAG-AausFP1-Z1_(planned 4005 bp ds-DNA     circular     05-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature                    19..1719
                                     /label="CAG Promoter"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     transcriptional terminator      complement(2743..2794)
                                     /label="T0 bacterial terminator"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature                    complement(2795..3176)
                                     /label="Zeocin resistance"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     misc_feature                    complement(3177..3242)
                                     /label="EM7 promoter"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
     polyA_signal                    2513..2704
                                     /label="SV40 late polyA"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     misc_feature                    2504..2737
                                     /label="SV40 poly(A)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature                    2486..2502
                                     /label="MCS"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     misc_feature                    1769..2473
                                     /label="AausFP1"
                                     /ApEinfo_revcolor=#9eafd2
                                     /ApEinfo_fwdcolor=#9eafd2
     primer                          1678..1697
                                     /label="pCAG_F_primer"
                                     /ApEinfo_revcolor=#c7b0e3
                                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature                    1763..1771
                                     /label="Kozak"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     regulatory                      84..371
                                     /label="CAG_enhancer"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     Bacterial origin of replication complement(3249..4005)
                                     /label="pMB1 ori"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature                    complement(7..18)
                                     /label="U6-gRNA-tRNA cloning site"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 CTGCAGAAGC TTTACTAACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA
       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC
      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG
      181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC
      241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG
      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG
      361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC
      421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA
      481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG
      541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT
      601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC
      661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC
      721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC
      781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG
      841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG
      901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG
      961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC
     1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG
     1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC
     1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC
     1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG
     1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT
     1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG
     1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC
     1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT
     1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG
     1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG
     1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA
     1681 ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCTGCAGT CGACGGTACC
     1741 GCGGGCCCGG GATCCACCGG TCGCCACCAT GGGTTCCTAT GGTGCTCTGC TGTTTAGGGA
     1801 GAAGATACCT TACGTGGTCG AAATGGAAGG CGACGTCGAA GGCATGAAAT TCAGCGTGCG
     1861 GGGCAAGGGG CACGGAGATG CCAATACAGG GAAGATCGAG GCTTCCTTTA TATGTACTAC
     1921 CGGCGAGCTG CCTGTTCCCT GGAGCTCTAT ACTCACCACC GTGACCTATG GTGCCCAATG
     1981 CTTCGCTAAG TACCCGAACG ACATAAAGGA TTATCCTAAG TCCGCAATGC CCGAGGGGTA
     2041 TGTCCAGGAA CGTACCATAA CATTCGAGAA TGATGGAGTT TACAAAACGC GGGCCGAGGT
     2101 TACTTATGAA AAGGGCTCCG TCTACAATAG GGTGACACTG AATGGGTCCG GGTTCAAGAA
     2161 GGGCGGAAAT ATTCTGGGAA AGAAGCTGGA GTTCAATTAT AATCCCCACT GTATTTACGT
     2221 CCTGCCCGAC GTTCAGAACA ACGGCATTAA GTGTTATATT AATATCGTGC ACGATGTGAT
     2281 CGGCGGCGGT CAGATCATCG CAGCCCATCA GCAGCTGAAC ACACCACTCG GCGGCGGTCC
     2341 CGTAGATATC CCGCATTATC ACCACATCCA GGCCCATACC ATCCTCTCAA AGGACCCGAA
     2401 GGAGACCAGG GACCACATGA ATGTGGTCGA AGTATTTCGT GCCATTGACT GTAAGACTGC
     2461 TTACGCCTGA TAGGCGGCCG CGACTCTAGA TGTACACATA TGGCTAGCTG GCCAGACATG
     2521 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
     2581 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
     2641 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT
     2701 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGAAT TCtggactca caaagaaaaa
     2761 acgcccggtg tgcaagaccg agcgttctga acaaTTTATC CTCAGTCCTG CTCCTCTGCC
     2821 ACAAAGTGCA CGCAGTTGCC GGCCGGGTCG CGCAGGGCGA ACTCCCGCCC CCACGGCTGC
     2881 TCGCCGATCT CGGTCATGGC CGGCCCGGAG GCGTCCCGGA AGTTCGTGGA CACGACCTCC
     2941 GACCACTCGG CGTACAGCTC GTCCAGGCCG CGCACCCACA CCCAGGCCAG GGTGTTGTCC
     3001 GGCACCACCT GGTCCTGGAC CGCGCTGATG AACAGGGTCA CGTCGTCCCG GACCACACCG
     3061 GCGAAGTCGT CCTCCACGAA GTCCCGGGAG AACCCGAGCC GGTCGGTCCA GAACTCGACC
     3121 GCTCCGGCGA CGTCGCGCGC GGTGAGCACC GGAACGGCAC TGGTCAACTT GGCCATGATG
     3181 GCCCTCCTAT AGTGAGTCGT ATTATACTAT GCCGATATAC TATGCCGATG ATTAATTGTC
     3241 AAGGATCCTA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     3301 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     3361 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     3421 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     3481 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     3541 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     3601 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     3661 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     3721 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG
     3781 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     3841 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     3901 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGAAAACT CACGTTAAGG
     3961 GATTTTGGTC ATGGCTAGTT AATTAACATT TAAATCAGCG GCCGC
//