LOCUS       CMV-AausFP1-Z1_(planned 2930 bp ds-DNA     circular     05-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature                    688..696
                                     /label="Kozak"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature                    complement(7..18)
                                     /label="U6-gRNA-tRNA cloning site"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     Bacterial origin of replication complement(2174..2930)
                                     /label="pMB1 ori"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind                     534..554
                                     /label="CMV-F"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     enhancer                        76..379
                                     /label="CMV enhancer"
                                     /ApEinfo_revcolor=#c6c9d1
                                     /ApEinfo_fwdcolor=#c6c9d1
     transcriptional terminator      complement(1668..1719)
                                     /label="T0 bacterial terminator"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal                    1438..1629
                                     /label="SV40 late polyA"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     misc_feature                    complement(1720..2101)
                                     /label="Zeocin resistance"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     misc_feature                    1429..1662
                                     /label="SV40 poly(A)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     source                          19..75
                                     /label="source"
                                     /ApEinfo_revcolor=#ffef86
                                     /ApEinfo_fwdcolor=#ffef86
     misc_feature                    694..1398
                                     /label="AausFP1"
                                     /ApEinfo_revcolor=#9eafd2
                                     /ApEinfo_fwdcolor=#9eafd2
     source                          76..660
                                     /label="source"
                                     /ApEinfo_revcolor=#ffef86
                                     /ApEinfo_fwdcolor=#ffef86
     misc_feature                    complement(2102..2167)
                                     /label="EM7 promoter"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature                    1411..1427
                                     /label="MCS"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     promoter                        380..583
                                     /label="CMV promoter"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature                    606..660
                                     /label="MCS"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CTGCAGAAGC TTTACTAAct agtaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      601 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
      661 GTACCGCGGG CCCGGGATCC ACCGGTCGCC ACCATGGGTT CCTATGGTGC TCTGCTGTTT
      721 AGGGAGAAGA TACCTTACGT GGTCGAAATG GAAGGCGACG TCGAAGGCAT GAAATTCAGC
      781 GTGCGGGGCA AGGGGCACGG AGATGCCAAT ACAGGGAAGA TCGAGGCTTC CTTTATATGT
      841 ACTACCGGCG AGCTGCCTGT TCCCTGGAGC TCTATACTCA CCACCGTGAC CTATGGTGCC
      901 CAATGCTTCG CTAAGTACCC GAACGACATA AAGGATTATC CTAAGTCCGC AATGCCCGAG
      961 GGGTATGTCC AGGAACGTAC CATAACATTC GAGAATGATG GAGTTTACAA AACGCGGGCC
     1021 GAGGTTACTT ATGAAAAGGG CTCCGTCTAC AATAGGGTGA CACTGAATGG GTCCGGGTTC
     1081 AAGAAGGGCG GAAATATTCT GGGAAAGAAG CTGGAGTTCA ATTATAATCC CCACTGTATT
     1141 TACGTCCTGC CCGACGTTCA GAACAACGGC ATTAAGTGTT ATATTAATAT CGTGCACGAT
     1201 GTGATCGGCG GCGGTCAGAT CATCGCAGCC CATCAGCAGC TGAACACACC ACTCGGCGGC
     1261 GGTCCCGTAG ATATCCCGCA TTATCACCAC ATCCAGGCCC ATACCATCCT CTCAAAGGAC
     1321 CCGAAGGAGA CCAGGGACCA CATGAATGTG GTCGAAGTAT TTCGTGCCAT TGACTGTAAG
     1381 ACTGCTTACG CCTGATAGGC GGCCGCGACT CTAGATGTAC ACATATGGCT AGCTGGCCAG
     1441 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
     1501 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
     1561 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG
     1621 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGAATTCtgg actcacaaag
     1681 aaaaaacgcc cggtgtgcaa gaccgagcgt tctgaacaaT TTATCCTCAG TCCTGCTCCT
     1741 CTGCCACAAA GTGCACGCAG TTGCCGGCCG GGTCGCGCAG GGCGAACTCC CGCCCCCACG
     1801 GCTGCTCGCC GATCTCGGTC ATGGCCGGCC CGGAGGCGTC CCGGAAGTTC GTGGACACGA
     1861 CCTCCGACCA CTCGGCGTAC AGCTCGTCCA GGCCGCGCAC CCACACCCAG GCCAGGGTGT
     1921 TGTCCGGCAC CACCTGGTCC TGGACCGCGC TGATGAACAG GGTCACGTCG TCCCGGACCA
     1981 CACCGGCGAA GTCGTCCTCC ACGAAGTCCC GGGAGAACCC GAGCCGGTCG GTCCAGAACT
     2041 CGACCGCTCC GGCGACGTCG CGCGCGGTGA GCACCGGAAC GGCACTGGTC AACTTGGCCA
     2101 TGATGGCCCT CCTATAGTGA GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA
     2161 TTGTCAAGGA TCCTAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
     2221 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     2281 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     2341 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     2401 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     2461 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     2521 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     2581 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     2641 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG
     2701 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     2761 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     2821 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGA AAACTCACGT
     2881 TAAGGGATTT TGGTCATGGC TAGTTAATTA ACATTTAAAT CAGCGGCCGC
//