LOCUS       CMV-eGFP-Z1_(planned)   2946 bp ds-DNA     circular     05-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter                        380..583
                                     /label="CMV promoter"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     source                          19..75
                                     /label="source"
                                     /ApEinfo_revcolor=#ffef86
                                     /ApEinfo_fwdcolor=#ffef86
     primer_bind                     534..554
                                     /label="CMV-F"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     misc_feature                    606..660
                                     /label="MCS"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal                    1454..1645
                                     /label="SV40 late polyA"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     Bacterial origin of replication complement(2190..2946)
                                     /label="pMB1 ori"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     primer                          complement(739..760)
                                     /label="EGFP_N_primer"
                                     /ApEinfo_revcolor=#b4abac
                                     /ApEinfo_fwdcolor=#b4abac
     gene                            694..1413
                                     /label="EGFP"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     misc_feature                    1427..1443
                                     /label="MCS"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     primer                          1347..1368
                                     /label="EGFP_C_primer"
                                     /ApEinfo_revcolor=#c6c9d1
                                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature                    1445..1678
                                     /label="SV40 poly(A)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     source                          76..660
                                     /label="source"
                                     /ApEinfo_revcolor=#ffef86
                                     /ApEinfo_fwdcolor=#ffef86
     transcriptional terminator      complement(1684..1735)
                                     /label="T0 bacterial terminator"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature                    complement(1736..2117)
                                     /label="Zeocin resistance"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     misc_feature                    688..696
                                     /label="Kozac"
                                     /ApEinfo_revcolor=#9eafd2
                                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature                    complement(7..18)
                                     /label="U6-gRNA-tRNA cloning site"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     misc_feature                    complement(2118..2183)
                                     /label="EM7 promoter"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
     feature                         877..906
                                     /label="Y66 (EGFP)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     enhancer                        76..379
                                     /label="CMV enhancer"
                                     /ApEinfo_revcolor=#c6c9d1
                                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 CTGCAGAAGC TTTACTAAct agtaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      601 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg
      661 GTACCGCGGG CCCGGGATCC Accggtcgcc accatggtga gcaagggcga ggagctgttc
      721 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      781 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      901 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      961 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1021 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1081 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1141 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1201 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1261 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1321 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1381 atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactCTAG ATGTACACAT
     1441 ATGGCTAGCT GGCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA
     1501 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC
     1561 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT
     1621 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGAA
     1681 TTCtggactc acaaagaaaa aacgcccggt gtgcaagacc gagcgttctg aacaaTTTAT
     1741 CCTCAGTCCT GCTCCTCTGC CACAAAGTGC ACGCAGTTGC CGGCCGGGTC GCGCAGGGCG
     1801 AACTCCCGCC CCCACGGCTG CTCGCCGATC TCGGTCATGG CCGGCCCGGA GGCGTCCCGG
     1861 AAGTTCGTGG ACACGACCTC CGACCACTCG GCGTACAGCT CGTCCAGGCC GCGCACCCAC
     1921 ACCCAGGCCA GGGTGTTGTC CGGCACCACC TGGTCCTGGA CCGCGCTGAT GAACAGGGTC
     1981 ACGTCGTCCC GGACCACACC GGCGAAGTCG TCCTCCACGA AGTCCCGGGA GAACCCGAGC
     2041 CGGTCGGTCC AGAACTCGAC CGCTCCGGCG ACGTCGCGCG CGGTGAGCAC CGGAACGGCA
     2101 CTGGTCAACT TGGCCATGAT GGCCCTCCTA TAGTGAGTCG TATTATACTA TGCCGATATA
     2161 CTATGCCGAT GATTAATTGT CAAGGATCCT AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     2221 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     2281 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     2341 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     2401 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     2461 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     2521 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     2581 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     2641 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     2701 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     2761 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     2821 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2881 CAGTGAAAAC TCACGTTAAG GGATTTTGGT CATGGCTAGT TAATTAACAT TTAAATCAGC
     2941 GGCCGC
//