LOCUS       Exported               10716 bp ds-DNA     circular SYN 17-OCT-2019
DEFINITION  Ligation of ova nde xma into g34r xma ndei.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-puro-cOVA-IRES-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2019 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    29..586
                     /label=IRES
                     /note="color: #a6acb3"
     CDS             599..1318
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  599 ..  601 / #3366ff
                        2:  602 ..  604 / #3366ff / 1a
                        3:  605 .. 1318 / #3366ff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     misc_feature    599..601
                     /label=Segment of EBFP2
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    602..604
                     /label=1a
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    1441..1562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #cc0000; direction: RIGHT"
     promoter        1650..2149
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2170..2769
                     /label=Puro(R)
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2783..3371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     LTR             3578..4211
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(4340..4356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    4364..4380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(4388..4418)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 4388 .. 4394 / #ffffff / -10
                        2: 4395 .. 4412 / #ffffff
                        3: 4413 .. 4418 / #ffffff / -35"
     protein_bind    4433..4454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(4742..5330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(5501..6361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5501 .. 6292 / #ccffcc
                        2: 6293 .. 6361 / #ccffcc / signal sequence
                     Cleavage site after base 6292"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6362..6466)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    6514..6648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             6816..7449
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7496..7621
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8118..8351
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    8843..8958
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
                     /note="color: #ffe4c4"
     enhancer        9016..9319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        9320..9531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     intron          9692..9824
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
                     /note="color: #------"
     promoter        9869..9887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9904..10686
                     /codon_start=1
                     /label=cOVA
                     /note="color: #993366"
                     /translation="MGSIGAASMEFCFDVFKELINSWVESQTNGIIRNVLQPSSVDSQT
                     AMVLVNAIVFKGLWEKTFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVASMASEKMKI
                     LELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKVYLPRMKME
                     EKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGREVVGSAEA
                     GVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
ORIGIN
        1 ccgggatccc aattgctcga gcgggatcaa ttccgccccc cccctaacgt tactggccga
       61 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
      121 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
      181 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
      241 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
      301 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
      361 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
      421 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
      481 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
      541 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
      601 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      661 cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg
      721 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      781 cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      841 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      901 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      961 gaaccgcatc gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa
     1021 gctggagtac aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg
     1081 catcaaggtg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
     1141 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta
     1201 cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1261 gctggagttc cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     1321 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     1381 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1441 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     1501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     1561 taatccggcg cgccatacgc gtatttaagg cgtaaattgt aagcgttaat gggatccacc
     1621 ggatctagat aactgatcat aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     1681 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     1741 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     1801 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     1861 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     1921 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     1981 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     2041 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     2101 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     2161 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     2221 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     2281 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     2341 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     2401 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     2461 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     2521 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     2581 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     2641 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     2701 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     2761 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     2821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     3181 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3301 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3361 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     3421 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     3481 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     3541 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     3601 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     3661 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     3721 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     3781 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     3841 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     3901 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     3961 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     4021 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4081 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4141 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4201 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4261 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     4321 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4381 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4441 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4501 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5161 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6481 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     6541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     6661 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     6721 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     6781 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     6841 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     6901 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     6961 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7021 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7081 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7141 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7201 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7261 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7441 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     7501 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     7561 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     7621 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     7681 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     7741 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     7801 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     7861 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     7921 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     7981 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8041 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8101 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     8161 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     8221 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     8281 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8341 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8401 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     8461 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8521 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8581 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8641 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8701 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     8761 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     8821 aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt acagtgcagg
     8881 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     8941 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttatcgata
     9001 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     9061 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     9121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     9181 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     9241 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     9301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     9361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     9421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tgcgatcgcc cgccccgttg
     9481 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     9541 aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat tgctaacgca
     9601 gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gtgactctct taaggtagcc
     9661 ttgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
     9721 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
     9781 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
     9841 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcctcgagaa
     9901 ttcatgggct ccatcggcgc agcaagcatg gaattttgtt ttgatgtatt caaggagctc
     9961 atcaattcct gggtagaaag tcagacaaat ggaattatca gaaatgtcct tcagccaagc
    10021 tccgtggatt ctcaaactgc aatggttctg gttaatgcca ttgtcttcaa aggactgtgg
    10081 gagaaaacat ttaaggatga agacacacaa gcaatgcctt tcagagtgac tgagcaagaa
    10141 agcaaacctg tgcagatgat gtaccagatt ggtttattta gagtggcatc aatggcttct
    10201 gagaaaatga agatcctgga gcttccattt gccagtggga caatgagcat gttggtgctg
    10261 ttgcctgatg aagtctcagg ccttgagcag cttgagagta taatcaactt tgaaaaactg
    10321 actgaatgga ccagttctaa tgttatggaa gagaggaaga tcaaagtgta cttacctcgc
    10381 atgaagatgg aggaaaaata caacctcaca tctgtcttaa tggctatggg cattactgac
    10441 gtgtttagct cttcagccaa tctgtctggc atctcctcag cagagagcct gaagatatct
    10501 caagctgtcc atgcagcaca tgcagaaatc aatgaagcag gcagagaggt ggtagggtca
    10561 gcagaggctg gagtggatgc tgcaagcgtc tctgaagaat ttagggctga ccatccattc
    10621 ctcttctgta tcaagcacat cgcaaccaac gccgttctct tctttggcag atgtgtttcc
    10681 ccttaagaat tcacgcgtgg tacctctaga gtcgac
//
