LOCUS       pJOE7771.1              6786 bp    DNA     circular SYN 08-DEC-2015
DEFINITION  Ligation of inverted PCR5751.1s8325/8326AgeI/BamHI into pZSU179.9AgeI/BamHI
ACCESSION   pJOE7771.1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             complement (6587..799)
                     /gene="mob"
     CDS             1793..2455
                     /gene="rep"
                     /product="Rep"
     CDS             complement (2762..3037)
                     /gene="rrnB'"
                     /product="rrnB"
     CDS             complement (3047..3796)
                     /gene="6His-eGFP"
                     /product="eGFP"
     CDS             complement (3819..3976)
                     /gene="mtlP"
                     /product="promoter des mtl-operons aus P.fluoresc."
     CDS             4124..5029
                     /gene="mtlR"
                     /product="orf3"
     CDS             5193..5232
                     /gene="ter"
     CDS             complement (5535..6329)
                     /gene="kanamycin"
                     /product="kanamycin resistance determinant"
ORIGIN      
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtgat
     2761 atcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     2821 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcatggggag accccacact
     2881 accatcggcg ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc
     2941 gctactgccg ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg
     3001 tatcaggctg aaaatcttct ctcatccgcc aaaacagcca agcttacttg tacagctcgt
     3061 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     3121 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     3181 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     3241 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     3301 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     3361 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     3421 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     3481 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     3541 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     3601 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     3661 cgccctcgcc gtacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     3721 tgggcaccac cccggtgaac agctcctcgc ccttgctgga tccatgatga tgatgatgat
     3781 gcgtaatcat ggtcatatgt atatctcctt cttaagaatt ccgcgcccag gggctacgga
     3841 aggacagtta ttgttgttgt gtcttcctac ggggagtcag gaagagtgtg cgttgattac
     3901 agccttcaaa cggggttgtg acaaatcctt gggagcactt ggactgatac ttttttgcac
     3961 tcgttaggta aacatcgatc tgctccacca tgcgcataaa agtacgcagt acatgtccca
     4021 gtggatcgag gcgaaaaacc gtagcctgta aaccgcggcg ctggcgaggt cgtgcgcgag
     4081 ggggcattat cccaagcagc aaccacaata aagcgacggc agcatgaccc gagcaacgcg
     4141 aatcaccgac ccttcctacg agctgatgga cgatcacaac ggcctgtcca tcatctatcg
     4201 ccagcatggt ttcccctgcc cgctggtgcg ctggcatttc cacaaggaat acgagcttca
     4261 cctgatcgtc gccagttccg gcaaggtgtt tattggcgac tatatcggca acttctatcc
     4321 ggagagcctg ttcctcacgg ggcccaacct gccccacaac tggatcagcc aggtggagga
     4381 agacgaagtg gtgcccaagc gcgacatgct ggtgaacttc accgatgagc tgttcgagca
     4441 gggcagccat atttttgccg agctcaagac cttgacgccc atgctggaac gcgcgcagtc
     4501 cggcattgag tttcgctgca agaaaaccat cgcccaggcc atgaccctga tgcagcgtat
     4561 cgaagacgcc cggggcatgg cgcgcctggg gcacttcttt atcctgctgg aagtgttgag
     4621 tagctgtgag gactaccagt tgctgtccgg cgtgaccacg ccgcaactgg cggatgaaca
     4681 tagtatcgac cgcaccaacc gcgcggtgga ctacattttc gcccactacg cgcgcgagtt
     4741 gcccctggag gaagtggcgg aacacctggg gatgaagccg acgtactttt cccgggtgtt
     4801 caagcaggcg accgggcgca cgtttatcga gtttgtaaat cggctgcgca tcagcaagtc
     4861 ctgcgagctt ctggccgatg gcgacaaggc cgtgacggat gtgtgtttcg agtcgggttt
     4921 caacaatatt tccaacttca accggcgctt tcagcagctc aaggggatga ccccctctca
     4981 ctaccgccgt ttggcagttc agcgtctgac ggaacaaaac ctggcctgac ccagcaccta
     5041 aacaggtgct tacaaagcgt gtcggcaatt cccaccccaa acaggaacgt ggaacaagcc
     5101 ccaggccact tagcgaagtg cctctctcgt ttaaccagca agaggggaac tcaatctacg
     5161 caggcatagg gtagttccat gatcctgcag ttaacgcatg caaaaacgcc ccggtgatct
     5221 cggggcgttt ttaacatgca taaaaactgt tgtaattcat taagcattct gccgacatgg
     5281 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     5341 tgcgtataat atttgcccat gggggtgggc gaagaactcc agcatgagat ccccgcgctg
     5401 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     5461 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     5521 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     5581 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     5641 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     5701 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     5761 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     5821 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     5881 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     5941 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     6001 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     6061 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     6121 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     6181 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     6241 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     6301 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     6361 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     6421 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     6481 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     6541 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat cccaggtggc
     6601 acttttcggg gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct
     6661 ggacttcccg ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct
     6721 tggcctgcat atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc
     6781 gaaggt
//
