LOCUS       pJOE6090.1              4266 bp    DNA     circular SYN 12-MAR-2008
DEFINITION  Ligation of 4613.1NdeI/HindIII into 5886.1NdeI/HindIII
ACCESSION   pJOE6090.1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    101..128
                     /gene="Strep-Tag"
     CDS             128..841
                     /gene="'eGFP"
                     /product="eGFP"
     CDS             851..1275
                     /gene="rrnB"
                     /product="rrnB"
     CDS             1367..2227
                     /gene="bla"
                     /product="bla"
     misc_feature    3022
                     /gene="ori"
                     /product="ori"
     CDS             complement (3416..3607)
                     /gene="rop"
     misc_feature    complement (3860..4119)
                     /gene="cer"
                     /product="cer"
     CDS             4147..4162
                     /gene="pT7"
                     /product="pT7"
     CDS             4181..1
                     /gene="rhaP"
                     /product="rhaP"
ORIGIN      
        1 gaattcaggc gctttttaga ctggtcgtag ggagaccaca acggtttccc tctagaaata
       61 attttcttaa gaaggagata tacatatgac catgattacg tggagccatc cgcagtttga
      121 aaagggatcc agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      181 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      241 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      301 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      361 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      421 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      481 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      541 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      601 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      661 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      721 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      781 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
      841 gtaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
      901 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
      961 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     1021 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     1081 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     1141 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     1201 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     1261 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1321 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1381 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1441 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1501 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1561 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     1621 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1681 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1741 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1801 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1861 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1921 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1981 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2041 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2101 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2161 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2221 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2281 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2341 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2401 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2461 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2521 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2581 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2641 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2701 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2761 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2821 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2881 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2941 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3001 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3061 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     3181 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt
     3241 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     3301 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3361 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3421 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     3481 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     3541 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     3601 tggtcacttg atgcctccgt gtaaggggga atttctgttc atgggggtaa tgataccgat
     3661 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     3721 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     3781 gggtcaatgc cagcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     3841 ccgcaaggaa tggtgcatgc tcgatggcta cgagggcaga cagtaagtgg atttaccata
     3901 atcccttaat tgtacgcacc gctaaaacgc gttcagcgcg atcacggcag cagacaggta
     3961 aaaatggcaa caaaccaccc taaaaactgc gcgatcgcgc ctgataaatt ttaaccgtat
     4021 gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc actgaagctg
     4081 ccatttttca tggtttcacc atcccagcga agggccatgc atgcatcgaa attaatacga
     4141 cgaaattaat acgactcact atagggcaat tgcgatcacc acaattcagc aaattgtgaa
     4201 catcatcacg ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa
     4261 ggtcgc
//
