LOCUS       pJOE6089.4              4270 bp    DNA     circular SYN 12-MAR-2008
DEFINITION  Ligation of 4623.1NdeI/HindIII into 5886.1NdeI/HindIII
ACCESSION   pJOE6089.4
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             86..799
                     /gene="eGFP"
                     /product="eGFP"
     CDS             806..829
                     /gene="strep"
     CDS             855..1279
                     /gene="rrnB"
                     /product="rrnB"
     CDS             1371..2231
                     /gene="bla"
                     /product="bla"
     misc_feature    3026
                     /gene="ori"
                     /product="ori"
     CDS             complement (3420..3611)
                     /gene="rop"
     misc_feature    complement (3864..4123)
                     /gene="cer"
                     /product="cer"
     CDS             4151..4166
                     /gene="pT7"
                     /product="pT7"
     CDS             4185..1
                     /gene="rhaP"
                     /product="rhaP"
ORIGIN      
        1 gaattcaggc gctttttaga ctggtcgtag ggagaccaca acggtttccc tctagaaata
       61 attttcttaa gaaggagata tacatatgag caagggcgag gagctgttca ccggggtggt
      121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      181 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      241 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      301 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      481 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      541 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      661 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      781 catggacgag ctgtacaagg gatcctggag ccatccgcag tttgaaaagt aacccgggct
      841 gcagccaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
      901 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
      961 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     1021 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     1081 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1141 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     1201 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     1261 tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1381 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1441 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1501 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1561 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     1621 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1681 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1741 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1801 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1861 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1921 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1981 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2041 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2101 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2161 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2221 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2281 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2341 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2401 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2461 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2521 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2581 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2641 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2701 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2761 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2821 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2881 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2941 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3001 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3061 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3121 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3181 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     3241 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     3301 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3361 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3421 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     3481 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     3541 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     3601 tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac
     3661 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     3721 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     3781 ctcagggtca atgccagcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     3841 gccgccgcaa ggaatggtgc atgctcgatg gctacgaggg cagacagtaa gtggatttac
     3901 cataatccct taattgtacg caccgctaaa acgcgttcag cgcgatcacg gcagcagaca
     3961 ggtaaaaatg gcaacaaacc accctaaaaa ctgcgcgatc gcgcctgata aattttaacc
     4021 gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa
     4081 gctgccattt ttcatggttt caccatccca gcgaagggcc atgcatgcat cgaaattaat
     4141 acgacgaaat taatacgact cactataggg caattgcgat caccacaatt cagcaaattg
     4201 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
     4261 agaaggtcgc
//
