LOCUS       pJOE5751.1              4260 bp    DNA     circular SYN 16-JAN-2012
DEFINITION  5427.5EcoNI/AfeI Circularized
ACCESSION   pJOE5751.1
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES             Location/Qualifiers
     CDS             86..835
                     /gene="6His-eGFP"
                     /product="eGFP"
     CDS             845..1269
                     /gene="rrnB"
                     /product="rrnB"
     CDS             1361..2221
                     /gene="bla"
                     /product="bla"
     misc_feature    3016
                     /gene="ori"
                     /product="ori"
     CDS             complement (3410..3601)
                     /gene="rop"
     misc_feature    complement (3854..4113)
                     /gene="cer"
                     /product="cer"
     CDS             4141..4156
                     /gene="pT7"
                     /product="pT7"
     CDS             4174..1
                     /gene="rhaP"
                     /product="rhaP"
ORIGIN      
        1 gaattcaggc gctttttaga ctggtcgtag ggagaccaca acggtttccc tctagaaata
       61 attttcttaa gaaggagata tacatatgac catgattacg catcatcatc atcatcatgg
      121 atccagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      181 cgacgtaaac ggccacaagt tcagcgtgta cggcgagggc gagggcgatg ccacctacgg
      241 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      301 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      361 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      421 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      481 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      541 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      601 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      661 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      721 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
      781 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
      841 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      901 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      961 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
     1021 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1081 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     1141 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     1201 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     1261 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     1621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2521 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2761 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3181 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc
     3241 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     3301 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3361 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3421 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     3481 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     3541 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     3601 cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac cgatgaaacg
     3661 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     3721 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
     3781 atgccagcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     3841 ggaatggtgc atgctcgatg gctacgaggg cagacagtaa gtggatttac cataatccct
     3901 taattgtacg caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg
     3961 gcaacaaacc accctaaaaa ctgcgcgatc gcgcctgata aattttaacc gtatgaatac
     4021 ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa gctgccattt
     4081 ttcatggttt caccatccca gcgaagggcc atgcatgcat cgaaattaat acgacgaaat
     4141 taatacgact cactataggg caattgcgat caccacaatt cagcaaattg tgaacatcat
     4201 cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg agaaggtcgc
//
