LOCUS       Exported                6499 bp ds-DNA     circular SYN 15-JUL-2005
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 27, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific & Educational 
            Software
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             25..1186
                     /codon_start=1
                     /gene="malE"
                     /product="malE"
                     /label=malE
                     /note="/SECDrawAs=Gene"
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGIEGR"
     CDS             1190..1209
                     /gene="6xhis"
                     /product="his-Tag"
                     /label=6xhis
                     /note="/SECDrawAs=Gene"
     CDS             1216..1473
                     /gene="smt3"
                     /product="smt3 ab AS13, mit NarI am Ende"
                     /label=smt3
                     /note="/SECDrawAs=Gene"
     CDS             1474..2190
                     /codon_start=1
                     /gene="eGFP"
                     /product="eGFP"
                     /label=eGFP
                     /note="/SECDrawAs=Gene"
                     /translation="ASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2197..2621
                     /gene="rrnB"
                     /product="rrnB"
                     /label=rrnB
                     /note="/SECDrawAs=Gene"
     CDS             2713..3573
                     /codon_start=1
                     /gene="bla"
                     /product="bla"
                     /label=bla
                     /note="/SECDrawAs=Gene"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    4368
                     /gene="ori"
                     /product="ori"
                     /label=ori
                     /note="/SECDrawAs=Marker"
     misc_feature    complement(5794..6053)
                     /gene="cer"
                     /product="cer"
                     /label=cer
                     /note="/SECDrawAs=Region"
     misc_feature    complement(6058..6317)
                     /gene="cer"
                     /product="cer"
                     /label=cer
                     /note="/SECDrawAs=Region"
     CDS             6345..6360
                     /gene="pT7"
                     /product="pT7"
                     /label=pT7
                     /note="/SECDrawAs=Gene"
     CDS             6377..6465
                     /gene="rhaP'"
                     /product="rhaP"
                     /label=rhaP'
                     /note="/SECDrawAs=Gene"
     CDS             6467..6498
                     /gene="'rhaP"
                     /product="rhaP"
                     /label='rhaP
                     /note="/SECDrawAs=Gene"
ORIGIN
        1 aattcttaag aaggagatat acatatgaaa actgaagaag gtaaactggt aatctggatt
       61 aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc
      121 ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg
      181 gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct
      241 caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg
      301 tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa
      361 gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag
      421 atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg
      481 caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat
      541 gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt
      601 ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc
      661 atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca
      721 tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag
      781 ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg
      841 aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa
      901 gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg
      961 gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg
     1021 aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc
     1081 agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaattc gagctcgaac
     1141 aacaacaaca ataacaataa caacaacctc gggatcgagg gaaggggatc acaccaccat
     1201 caccatcacc gatccgaggt caagccagaa gtcaagcctg agactcacat caatttaaag
     1261 gtgtccgatg gatcttcaga gatcttcttc aagatcaaga aaaccacccc actgcgtcgg
     1321 ctgatggaag cgttcgctaa acgtcagggt aaagagatgg acagcctgcg ctttctgtat
     1381 gatggcattc gcattcaggc ggatcagacc ccggaagacc tggacatgga agataacgat
     1441 atcattgagg cccatcgtga acagattggt ggcgccagca agggcgagga gctgttcacc
     1501 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1561 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1621 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1681 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1741 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1801 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1861 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1921 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1981 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2041 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     2101 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2161 actctcggca tggacgagct gtacaagtaa gcttggctgt tttggcggat gagagaagat
     2221 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     2281 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     2341 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     2401 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2461 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     2521 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     2581 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     2641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     2761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2941 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3421 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     3601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4501 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4561 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4621 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4681 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4741 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     4801 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     4861 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     4921 catgttaagg gcggtttttt cctgtttggt cacttgatgc ctccgtgtaa gggggaattt
     4981 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     5041 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     5101 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     5161 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     5221 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     5281 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     5341 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     5401 agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg
     5461 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     5521 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     5581 cgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     5641 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     5701 tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     5761 gttgagcacc gccgccgcaa ggaatggtgc atgctcgatg gctacgaggg cagacagtaa
     5821 gtggatttac cataatccct taattgtacg caccgctaaa acgcgttcag cgcgatcacg
     5881 gcagcagaca ggtaaaaatg gcaacaaacc accctaaaaa ctgcgcgatc gcgcctgata
     5941 aattttaacc gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta
     6001 atccactgaa gctgccattt ttcatggttt caccatccca gcgaagggcc atgcatgctc
     6061 gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg tacgcaccgc
     6121 taaaacgcgt tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccta
     6181 aaaactgcgc gatcgcgcct gataaatttt aaccgtatga atacctatgc aaccagaggg
     6241 tacaggccac attaccccca cttaatccac tgaagctgcc atttttcatg gtttcaccat
     6301 cccagcgaag ggccatgcat gcatcgaaat taatacgacg aaattaatac gactcactat
     6361 agggcaattg cgatcaccac aattcagcaa attgtgaaca tcatcacgtt catctttccc
     6421 tggttgccaa tggcccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt
     6481 tagactggtc gtaatgaac
//
