LOCUS       Exported                7117 bp ds-DNA     circular SYN 19-JUN-2008
DEFINITION  HindIII/EcoRI, Ligation of inverted Fragment 2 - 4056 into Fragment 
            2 - 4782.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 27, 2019 from SnapGene Viewer 4.3.11
            https://www.snapgene.com
COMMENT     SECID/File created by SciEd Central, Scientific & Educational 
            Software
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1793..2455
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="/SECDrawAs=Gene"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             complement(2765..3478)
                     /gene="'eGFP"
                     /product="eGFP"
                     /label='eGFP
                     /note="/SECDrawAs=Gene"
     CDS             complement(3540..3571)
                     /codon_start=1
                     /gene="rhaP"
                     /product="rhaP"
                     /label=rhaP
                     /note="/SECDrawAs=Gene"
                     /translation="IQALFRLVVM"
     CDS             3809..4645
                     /codon_start=1
                     /gene="rhaS"
                     /product="rhaS"
                     /label=rhaS
                     /note="/SECDrawAs=Gene"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             4629..5567
                     /codon_start=1
                     /gene="rhaR"
                     /product="rhaR"
                     /label=rhaR
                     /note="/SECDrawAs=Gene"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFAS
                     DQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKH
                     SYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFNASAGQPHWRLGSMGMAQARQVIGQL
                     EHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSP
                     FALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTEC
                     GFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     CDS             complement(5866..6660)
                     /gene="kanamycin"
                     /product="kanamycin resistance determinant"
                     /label=kanamycin
                     /note="/SECDrawAs=Gene"
     CDS             complement(join(6918..7117,1..799))
                     /gene="mob"
                     /label=mob
                     /note="/SECDrawAs=Gene"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaag
     2761 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     2821 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     2881 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     2941 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     3001 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     3061 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     3121 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     3181 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     3241 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     3301 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     3361 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     3421 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctggatc
     3481 catgatgatg atgatgatgc gtaatcatgg tcatatgtat atctccttct taagaattgt
     3541 tcattacgac cagtctaaaa agcgcctgaa ttcgcgacct tctcgttact gacaggaaaa
     3601 tgggccattg gcaaccaggg aaagatgaac gtgatgatgt tcacaatttg ctgaattgtg
     3661 gtgatgtgat gctcaccgca tttcctgaaa attcacgctg tatcttgaaa aatcgacgtt
     3721 ttttacgtgg ttttccgtcg aaaatttaag gtaagaacct gacctcgtga ttactatttc
     3781 gccgtgttga cgacatcagg aggccagtat gaccgtatta catagtgtgg atttttttcc
     3841 gtctggtaac gcgtccgtgg cgatagaacc ccggctcccg caggcggatt ttcctgaaca
     3901 tcatcatgat tttcatgaaa ttgtgattgt cgaacatggc acgggtattc atgtgtttaa
     3961 tgggcagccc tataccatca ccggtggcac ggtctgtttc gtacgcgatc atgatcggca
     4021 tctgtatgaa cataccgata atctgtgtct gaccaatgtg ctgtatcgct cgccggatcg
     4081 atttcagttt ctcgccgggc tgaatcagtt gctgccacaa gagctggatg ggcagtatcc
     4141 gtctcactgg cgcgttaacc acagcgtatt gcagcaggtg cgacagctgg ttgcacagat
     4201 ggaacagcag gaaggggaaa atgatttacc ctcgaccgcc agtcgcgaga tcttgtttat
     4261 gcaattactg ctcttgctgc gtaaaagcag tttgcaggag aacctggaaa acagcgcatc
     4321 acgtctcaac ttgcttctgg cctggctgga ggaccatttt gccgatgagg tgaattggga
     4381 tgccgtggcg gatcaatttt ctctttcact gcgtacgcta catcggcagc ttaagcagca
     4441 aacgggactg acgcctcagc gatacctgaa ccgcctgcga ctgatgaaag cccgacatct
     4501 gctacgccac agcgaggcca gcgttactga catcgcctat cgctgtggat tcagcgacag
     4561 taaccacttt tcgacgcttt ttcgccgaga gtttaactgg tcaccgcgtg atattcgcca
     4621 gggacgggat ggctttctgc aataacgcga atcttctcaa cgtatttgta cgccatattg
     4681 cgaataatca acttcgttct ctggccgagg tagccacggt ggcgcatcag ttaaaacttc
     4741 tcaaagatga tttttttgcc agcgaccagc aggcagtcgc tgtggctgac cgttatccgc
     4801 aagatgtctt tgctgaacat acacatgatt tttgtgagct ggtgattgtc tggcgcggta
     4861 atggcctgca tgtactcaac gatcgccctt atcgcattac ccgtggcgat ctcttttaca
     4921 ttcatgctga cgataaacac tcctacgctt ccgttaacga tctggttttg cagaatatta
     4981 tttattgccc ggagcgtctg aagctgaatc ttgactggca gggggcgatt ccgggattta
     5041 acgccagcgc agggcaacca cactggcgct taggtagcat ggggatggcg caggcgcggc
     5101 aggttatcgg tcagcttgag catgaaagta gtcagcatgt gccgtttgct aacgaaatgg
     5161 ctgagttgct gttcgggcag ttggtgatgt tgctgaatcg ccatcgttac accagtgatt
     5221 cgttgccgcc aacatccagc gaaacgttgc tggataagct gattacccgg ctggcggcta
     5281 gcctgaaaag tccctttgcg ctggataaat tttgtgatga ggcatcgtgc agtgagcgcg
     5341 ttttgcgtca gcaatttcgc cagcagactg gaatgaccat caatcaatat ctgcgacagg
     5401 tcagagtgtg tcatgcgcaa tatcttctcc agcatagccg cctgttaatc agtgatattt
     5461 cgaccgaatg tggctttgaa gatagtaact atttttcggt ggtgtttacc cgggaaaccg
     5521 ggatgacgcc cagccagtgg cgtcatctca attcgcagaa agattaatgc ataaaaactg
     5581 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     5641 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tgggggtggg
     5701 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     5761 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca
     5821 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc
     5881 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     5941 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     6001 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     6061 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     6121 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     6181 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     6241 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     6301 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     6361 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     6421 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     6481 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     6541 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     6601 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     6661 gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct
     6721 tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc
     6781 cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca
     6841 tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat
     6901 agcccagtag ctgacattca tcccaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt
     6961 tcctgctggc gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt
     7021 tcgcccacgg ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc
     7081 agggcgtcca gaacgggctt caggcgctcc cgaaggt
//
