LOCUS       pSB1C3-pT7-sfGFP_RED20  2741 bp ds-DNA     circular     18-OCT-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     stem_loop       1842..1876
                     /label="Stem loop"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     cds             complement(231..890)
                     /label="Chloramphenicol resistance"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     barcode         1788..1812
                     /label="Help:Barcodes"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1788..1812
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1057..1063
                     /label="RBS?"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_binding  1004..1023
                     /label="Verification forward (VF2) primer binding site"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     stem_loop       113..218
                     /label="T0 terminator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1028..1044
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    113..138
                     /label="CamR REV primer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_binding  complement(1969..1988)
                     /label="Verification reverse (VR) primer binding site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     dna             1813..1833
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc            2088..2088
                     /label="ORI"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     stem_loop       1834..1905
                     /label="E. coli his operon terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1003..1024)
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc            complement(2073..2687)
                     /label="rep (pMB1)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1064..1070
                     /label="FC013A Spacer"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(891..996)
                     /label="Unnanotated Promoter (P1004)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(1890..1914)
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(975..996)
                     /label="CamR FOR primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Reporter        1071..1787
                     /label="sfGFP-RED20"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
       61 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
      121 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      181 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      241 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      301 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      361 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      421 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
      481 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
      541 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
      601 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
      661 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
      721 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
      781 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
      841 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
      901 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
      961 tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtGCCACCTG ACGTCTAAGA
     1021 AACCgaataa tacgactcac tatagggctt aagtatAAGG AGAtatacat ATGTCAAAAG
     1081 GAGAAGAACT ATTCACCGGA GTCGTCCCGA TCCTAGTCGA ACTAGACGGA GACGTCAACG
     1141 GACACAAATT CTCAGTCCGT GGAGAAGGAG AAGGAGACGC AACCAACGGA AAACTAACCC
     1201 TAAAATTCAT CTGCACCACC GGAAAACTAC CGGTCCCGTG GCCGACCCTA GTCACCACCC
     1261 TAACCTACGG AGTCCAGTGC TTCTCACGTT ACCCGGACCA CATGAAACGT CACGACTTCT
     1321 TCAAATCAGC AATGCCGGAA GGATACGTCC AGGAACGTAC CATCTCATTC AAAGACGACG
     1381 GAACCTACAA AACCCGTGCA GAAGTCAAAT TCGAAGGAGA CACCCTAGTC AACCGTATCG
     1441 AACTAAAAGG AATCGACTTC AAAGAAGACG GAAACATCCT AGGACACAAA CTAGAATACA
     1501 ACTTCAACTC ACACAACGTC TACATCACCG CAGACAAACA GAAAAACGGA ATCAAAGCAA
     1561 ACTTCAAAAT CCGTCACAAC GTCGAAGACG GATCAGTCCA GCTAGCAGAC CACTACCAGC
     1621 AGAACACCCC GATCGGAGAC GGACCGGTCC TACTACCGGA CAACCACTAC CTATCAACCC
     1681 AGTCAGTCCT ATCAAAAGAC CCGAACGAAA AACGTGACCA CATGGTCCTA CTAGAATTCG
     1741 TCACCGCAGC AGGAATCACC CACGGAATGG ACGAACTATA CAAATGACGC TGATAGTGCT
     1801 AGTGTAGATC gctactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
     1861 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     1921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     2101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2521 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt t
//