LOCUS       Exported               10990 bp ds-DNA     circular SYN 19-JUL-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Guida Ruas
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Oct 21, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2841..5033
                     /label=MmTPC2
     CDS             5043..5750
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNLAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     promoter        5779..6278
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6299..6898
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6912..7500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7383..7394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7708..8341
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8470..8486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8494..8510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8518..8548)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8563..8584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8872..9457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9628..10488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10489..10593)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10641..10775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tagctagagg atcgctagcg ctaccggact cagatctcga
     2821 gctcaagctt cgaattcacc atggcggcag aagagcagcc ccttctgggc cggggccgag
     2881 gcagtggcca ggtccactcc ggtgctgcag ccgatcagga gctctgcata gaccaggctg
     2941 tggtcttcat tgaagacgcc ataaagtacc gctccatcta ccaccgcatg gatgctggtt
     3001 cattgtggct ttaccgctgg tattactcga acgtctgcca acgtgtgctg ggcttcatca
     3061 ttttcctgat cctgatcttg gcttttgtgg aggtcccatc ttccttcaca aagactgcag
     3121 atgtgcgcta ccgttcccag ccctggcagc cgccctgtgg cctgaccgag acgatcgagg
     3181 cgttctgctt gcttgccttc ctggttgacc tctctgtgaa gggctacttg gttgggcagg
     3241 cccagttgca gcagaacctg tggctgctgg cctactttat ggtgctggtt gtgtctgtgg
     3301 tggactggat cgtttcgctg agccttgctt gtgaggagcc cctgcggatg cgccggctgc
     3361 tccgcccctt cttcctgctg cagaattcct ccatgatgaa gaagaccctg aagtgtatac
     3421 ggtggtcgct accagaaatg gccagtgtcg ggctgctgct ggccatccac ctgtgtctct
     3481 tcaccatcat tgggatgctg ctgttcacca ttggtgagaa ggatgaagca caggaccagg
     3541 agaggctggc ctacttccgg aaccttccag aagcactgac ctcactccta gtgctgctga
     3601 cgacttccaa caaccctgat gtgatgatcc ctgcgtatac tcagaaccgg gcctttgctc
     3661 tcttcttcat agtcttcacc ttgataggaa gcttgtttct gatgaacctg ctgacagcca
     3721 tcatctataa tcagttccgt ggctacctaa tgaaatctct acagacctca ctgttccggc
     3781 ggcgactggg ggcccgtgca gcctacgaag tcctcgcctc cagggccggg ccagctggaa
     3841 ccacccctga gttagttggg gtgaatcctg agaccttcct gccagtgctt cagaaaacgc
     3901 agctgaacaa aacccacaaa caagccatta tgcagaaggt gcagtcctat gaaggccgcc
     3961 caatgctggc tgatgagttc cagaaactct tcgatgaggt tgacaaaggt gtggccaaag
     4021 agcgcccgct gaagccccaa taccagtctc catttctgca gactgcccag ttcatcttta
     4081 gccaccacta cttcgactac ctggggaacc tcgtcgctct gggaaacctc ttgtctattt
     4141 gtgtgttcct ggtgctggac tcagacctgc tgcctgggga acgtgatgac tttgtcctgg
     4201 ggattcttga ctacatcttc atcttgtact acctgctgga gctgctgttt aaggtgttcg
     4261 cgctgggcct gccaggctac ctgtcctacc atagcaatgt gtttgatggc ctcctcacca
     4321 tcatcctgct ggtttcggag atttgcaccc tggctgtata ccgattgccg cactcaggat
     4381 ggaagccaga gcagtatggc ccactgtcgc tctgggacat gacacgactg atgaacacac
     4441 tgattgtgtt tcgcttcctg cgcatcatcc ccaacataaa gccaatggct gaggtagcca
     4501 acaccatcct gggcctaatc cctaacttga gggcatttgg tgggatcctg gtggtggcat
     4561 actatgtgtt tgccatgatc gggatcaacc tgttccgagg tgtcatcgtg cctcctggaa
     4621 acagcagcct ggttcccgac aacaactcag ctgtatgtgg aagctttgag cagctaggtt
     4681 actggcccaa caactttgac gactttgctg ctgctttgat cacactgtgg aacgtgatgg
     4741 tggtgaacaa ttggcaggtg atcctggaag cctataagcg ctacgcaggc ccgtggtcaa
     4801 tggtgtattt tgtgctatgg tggctggtgt cctctgttat ctggatcaac ctgtttctgg
     4861 ctcttcttct ggagaacttt ctccacagat gggaccctca aggtcataag cagctccttg
     4921 ttgggaccaa gcagatgtct gtggagctca tgttcaggga tatcctagaa gagcccaagg
     4981 aggaggaact gatggagaag ctacacaaac acccacactt gcatctgtgc agggcggccg
     5041 cggtgagcaa gggcgaggag gataacttag ccatcatcaa ggagttcatg cgcttcaagg
     5101 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     5161 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     5221 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     5281 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     5341 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     5401 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     5461 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     5521 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     5581 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     5641 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     5701 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag ggatcccgcg
     5761 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5821 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5881 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     5941 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6001 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6061 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6121 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6181 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6241 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     6901 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6961 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7021 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7081 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7141 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7201 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7261 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7321 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7381 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7441 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7501 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     7561 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     7621 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     7681 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     7741 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     7801 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     7861 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     7921 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     7981 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     8041 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     8101 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     8161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8341 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8401 gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc
     8461 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8521 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8581 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8641 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8701 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8761 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8821 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8881 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8941 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9001 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9061 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9121 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9181 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9241 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9301 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9361 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     9421 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9481 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9541 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9601 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9661 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9721 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9781 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9841 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9901 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9961 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10021 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10081 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10141 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10201 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10261 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10321 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10381 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10441 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10501 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10561 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10621 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    10681 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10741 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10801 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
    10861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    10921 atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata tgtactacaa
    10981 acttagtagt
//
