LOCUS       Exported                3004 bp ds-DNA     circular SYN 25-OCT-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Oct 25, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             106..765
                     /codon_start=1
                     /product="secreted Metridia luciferase"
                     /label=MetLuc
                     /note="secreted Metridia luciferase drosophilized"
                     /note="This feature has 2 segments:
                        1: 106 .. 156 / #00ffff / Signal Sequence
                        2: 157 .. 765 / #00ffff
                     Cleavage site after base 156"
                     /translation="MDIKVVFTLVFSALVQAKSTEFDPNIDIVGLEGKFGITNLETDLF
                     TIWETMEVMIKADIADTDRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTR
                     GCLICLSKIKCTAKMKVYIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQF
                     IAQVDRCASCTTGCLKGLANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     promoter        complement(806..824)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(840..856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(953..1635)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     promoter        1674..1772
                     /label=cat Promoter
                     /note="color: #ffffff"
     CDS             1777..2433
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /note="color: #ff6600"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2437..2544
                     /note="color: #ffffff"
     rep_origin      2549..3004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcgaatgga catcaaagtc
      121 gtgttcacgc tggtgttttc cgcgctcgta caagctaaat ccacagaatt tgacccgaac
      181 attgatattg tcggcctgga gggaaaattc ggtataacca acttggagac agatctcttt
      241 acgatttggg agacaatgga ggtcatgata aaggcggaca tagcagatac agatagggca
      301 agtaattttg tagcaaccga aacggacgct aaccgtggca aaatgcccgg aaagaagctc
      361 cctttggccg taataatgga gatggaggct aatgccttta aagccggatg tactcgtggc
      421 tgcctgattt gcttgagcaa gatcaaatgt acagccaaaa tgaaggtgta tatacccgga
      481 cggtgtcacg actatggcgg agataaaaag acgggacagg caggaatagt gggtgctata
      541 gtagatatac cagaaattag cggatttaaa gagatggcac caatggaaca attcattgct
      601 caagtagacc gatgcgccag ctgcacgacg ggctgtttga agggattggc aaatgttaag
      661 tgtagcgaac tcctgaaaaa atggctgcct gaccgctgtg cctccttcgc ggataagata
      721 cagaaggagg tccataatat caagggcatg gcaggagata ggtaggcttc gagaccactc
      781 atcgcccaat cactagtgaa ttcgttctat agtgtcacct aaataccata gagccgttgg
      841 tcatagctgt ttcctgtcca ggcatcatga cacggttatc cacagaatca ggggataacg
      901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg tgatgatcgg
     1681 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
     1741 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1801 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1861 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1921 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1981 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2041 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2101 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2161 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt gtcagccaat
     2221 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2281 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2341 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2401 caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     2461 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     2521 tcgatgataa gctgtcaaac atgatgttac gcgccctgta gcggcgcatt aagcgcggcg
     2581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2761 gattagggtg atggttcacg tagtgggcca gcgccctgat agacggtttt tcgccctttg
     2821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2941 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3001 attt
//
