LOCUS       Exported                5873 bp ds-DNA     circular SYN 02-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5_FRT_TO_eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Nicole Hustedt
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 04, 2015 from SnapGene Viewer 2.7.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7775
                     /dnas_title="pcDNA5_FRT_TO_eGFP-C17ORF53"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    820..838
                     /dnas_title="tetO"
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /dnas_title="tetO"
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             990..1706
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     polyA_signal    3733..3957
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4241..4288
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4296..5318
                     /dnas_title="aph(4)-Ia"
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYH
                     WQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE
                     AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQS
                     LVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPST
                     RPRAKE"
     CDS             4296..5318
                     /dnas_title="hygromycin B phosphotransferase"
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYH
                     WQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSE
                     AMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQS
                     LVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPST
                     RPRAKE"
     polyA_signal    5448..5569
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5618..5634)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5642..5658
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5666..5696)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5711..5732
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
     rep_origin      complement(6020..6608)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6779..7639)
                     /dnas_title="bla"
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(7640..7744)
                     /dnas_title="bla"
                     /gene="bla"
                     /note="AmpR promoter"
     exon            1716..3656
                     /note="C17ORF53"
     exon            990..3657
                     /note="GFP-C17ORF53"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGAPACSLQKLFAVEEEFEDEDFLSAVEDAENRFTGSL
                     PVNAGRLRPVSSRPQETVQAQSSRLLLLHPTAPSEALGLPDLDLCLPASSTPSADSRP
                     SCIGAAPLRPVSTSSSWIGNQRRVTVTEVLRETARPQSSALHPLLTFESQQQQVGGFE
                     GPEQDEFDKVLASMELEEPGMELECGVSSEAIPILPAQQREGSVLAKKARVVDLSGSC
                     QKGPVPAIHKAGIMSAQDESLDPVIQCRTPRPPLRPGAVGHLPVPTALTVPTQQLHWE
                     VCPQRSPVQALQPLQAARGTIQSSPQNRFPCQPFQSPSSWLSGKAHLPRPRTPNSSCS
                     TPSRTSSGLFPRIPLQPQAPVSSIGSPVGTPKGPQGALQTPIVTNHLVQLVTAASRTP
                     QQPTHPSTRAKTRRFPGPAGILPHQQSGRSLEDIMVSAPQTPTHGALAKFQTEIVASS
                     QASVEEDFGRGPWLTMKSTLGLDERDPSCFLCTYSIVMVLRKQAALKQLPRNKVPNMA
                     VMIKSLTRSTMDASVVFKDPTGEMQGTVHRLLLETCQNELKPGSVLLLKQIGVFSPSL
                     RNHYLNVTPNNLVHIYSPDSGDGSFLKPSQPFPKDSGSFQHDVAAKPEEGFRTAQNLE
                     AEASPEEELPEADDLDGLLSELPEDFFCGTSS"
     PCR_primer      complement(2293..2312)
                     /dnas_title="GGCTTTTTTAGCCAATACTG"
                     /pair=""
                     /primer="GGCTTTTTTAGCCAATACTG"
                     /current=0
     PCR_primer      2313..2332
                     /dnas_title="CGGGTAGTTGATCTGAGTGG"
                     /pair=""
                     /primer="CGGGTAGTTGATCTGAGTGG"
                     /current=0
     PCR_primer      complement(2971..2990)
                     /dnas_title="GGTTGGAGTTTGGGGCGCGG"
                     /pair=""
                     /primer="GGTTGGAGTTTGGGGCGCGG"
                     /current=0
     PCR_primer      2991..3011
                     /dnas_title="CATGGTGCTCTGGCTAAATTC"
                     /pair=""
                     /primer="CATGGTGCTCTGGCTAAATTC"
                     /current=0
     PCR_primer      complement(3433..3452)
                     /dnas_title="CCCATCCCCAGAATCCGGGC"
                     /pair=""
                     /primer="CCCATCCCCAGAATCCGGGC"
                     /current=1
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag ctgttcaccg
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1081 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1201 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1261 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1441 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1501 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1561 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1621 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1681 ctctcggcat ggacgagctg tacaagggcg cgccaGCGTG CAGTTTGCAG AAGCTGTTTG
     1741 CTGTGGAAGA GGAGTTTGAA GATGAGGATT TCTTGTCTGC TGTGGAGGAT GCAGAGAACC
     1801 GGTTTACTGG CTCACTGCCT GTGAATGCTG GGCGCCTGAG ACCTGTCTCT TCTAGGCCAC
     1861 AGGAGACTGT GCAGGCACAG TCCTCCAGGC TGCTGCTGTT ACACCCCACT GCTCCCTCAG
     1921 AGGCTTTGGG CCTGCCAGAC TTGGACCTCT GCCTCCCTGC CTCCAGCACG CCCAGTGCTG
     1981 ACAGCCGTCC ATCATGCATA GGAGCAGCTC CCCTAAGGCC TGTCTCTACT TCCAGCAGCT
     2041 GGATTGGCAA TCAGAGAAGA GTGACAGTGA CAGAAGTGCT CAGAGAGACA GCAAGACCTC
     2101 AGTCCTCAGC CTTACACCCC CTACTCACCT TTGAGAGCCA ACAGCAGCAA GTTGGTGGCT
     2161 TTGAGGGGCC TGAACAAGAC GAATTTGATA AAGTCCTGGC AAGCATGGAG TTGGAGGAGC
     2221 CTGGCATGGA GCTGGAATGT GGAGTCAGCA GTGAGGCCAT ACCAATCCTG CCTGCCCAGC
     2281 AGCGGGAGGG TTCAGTATTG GCTAAAAAAG CCCGGGTAGT TGATCTGAGT GGATCTTGCC
     2341 AGAAGGGGCC TGTGCCTGCC ATCCACAAAG CGGGTATCAT GTCCGCCCAG GATGAGTCTC
     2401 TAGATCCTGT CATCCAATGT AGGACTCCAC GACCCCCCTT GAGACCTGGT GCTGTGGGTC
     2461 ACCTTCCTGT TCCAACTGCC TTAACAGTTC CCACTCAGCA ACTCCACTGG GAAGTCTGTC
     2521 CGCAACGCTC CCCTGTTCAA GCACTTCAGC CTCTCCAAGC TGCTAGAGGG ACCATTCAGA
     2581 GCAGCCCTCA AAATCGTTTC CCTTGTCAGC CATTCCAGTC TCCAAGTTCC TGGTTAAGTG
     2641 GCAAAGCTCA TTTACCCAGA CCTCGAACTC CCAACTCAAG CTGTTCTACT CCCTCAAGGA
     2701 CTAGCTCTGG ATTATTTCCT CGGATACCCT TACAACCGCA AGCTCCAGTG TCTTCCATTG
     2761 GGTCTCCTGT TGGTACCCCA AAAGGTCCCC AGGGAGCTCT GCAGACACCC ATAGTCACCA
     2821 ACCACCTGGT GCAGCTAGTC ACTGCTGCCA GCCGGACACC CCAGCAGCCC ACCCATCCCT
     2881 CCACCCGAGC CAAAACTCGC CGTTTCCCTG GCCCAGCTGG GATCCTGCCT CACCAGCAGA
     2941 GTGGGAGAAG TCTGGAGGAC ATCATGGTTT CCGCGCCCCA AACTCCAACC CATGGTGCTC
     3001 TGGCTAAATT CCAGACAGAG ATTGTTGCTA GTTCCCAGGC ATCTGTGGAG GAGGATTTTG
     3061 GGCGAGGGCC CTGGCTGACC ATGAAATCCA CGCTAGGCCT GGATGAGAGA GACCCTAGCT
     3121 GCTTCCTCTG TACCTACAGC ATTGTCATGG TGCTGCGCAA GCAGGCAGCC CTGAAGCAGC
     3181 TTCCTAGGAA CAAGGTCCCC AACATGGCGG TGATGATCAA GTCCCTGACT CGGAGCACAA
     3241 TGGACGCCAG TGTGGTTTTC AAGGACCCCA CGGGAGAGAT GCAGGGGACG GTGCACAGGT
     3301 TGCTGCTGGA GACGTGCCAG AATGAGCTGA AGCCTGGCTC AGTGCTGCTG CTGAAGCAGA
     3361 TTGGAGTGTT TTCTCCTTCA CTTCGAAATC ACTACCTCAA CGTGACACCC AACAACCTGG
     3421 TCCATATTTA CAGCCCGGAT TCTGGGGATG GGAGCTTCCT CAAGCCATCT CAGCCCTTCC
     3481 CCAAGGATTC AGGGAGCTTC CAGCATGATG TGGCTGCAAA GCCCGAGGAA GGCTTCAGAA
     3541 CAGCACAGAA CCTAGAGGCA GAGGCGTCCC CTGAGGAAGA ACTCCCAGAA GCAGATGACC
     3601 TGGATGGACT CCTGAGTGAG CTTCCTGAAG ACTTCTTCTG TGGGACCAGT AGTTGAgaat
     3661 tctgcagata tccatcacac tggcggccgc tcgagtctag agggcccgtt taaacccgct
     3721 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3781 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3841 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3901 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3961 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4021 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4081 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4141 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4201 accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta ttccgaagtt
     4261 cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc accgcgacgt
     4321 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
     4381 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
     4441 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
     4501 ccgcgctccc gattccggaa gtgcttgaca ttggggattt cagcgagagc ctgacctatt
     4561 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
     4621 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
     4681 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
     4741 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
     4801 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
     4861 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
     4921 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
     4981 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
     5041 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
     5101 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
     5161 agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
     5221 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
     5281 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga gatttcgatt
     5341 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5401 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5581 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5641 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5701 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5761 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5821 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7741 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//

