LOCUS       pB_35S_mEGFP            9966 bp ds-DNA     circular SYN 24-OCT-2019
DEFINITION  Expression of mEGFP controlled by the CaMV 35S promoter.
ACCESSION
VERSION     .
KEYWORDS    pN_35S/mCitrine.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Pribil and Mathias
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid
            protein CURT1A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        1..345
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
                     /standard_name="CaMV 35S promoter"
     primer_bind     289..309
                     /note="CaMV 35S promoter, forward primer"
                     /standard_name="35S promoter"
     CDS             453..1172
                     /standard_name="mEGFP"
     misc_feature    complement(498..519)
                     /standard_name="EGFP_N_primer"
     misc_feature    636..665
                     /standard_name="Y66 (EGFP)"
     misc_feature    1106..1127
                     /standard_name="EGFP_C_primer"
     protein_bind    complement(1175..1199)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /standard_name="attB2"
     terminator      1209..1434
                     /standard_name="35S terminator"
     misc_feature    1519..1543
                     /note="right border repeat from nopaline C58 T-DNA"
                     /standard_name="RB T-DNA repeat"
     CDS             2874..3503
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
                     /standard_name="pVS1 StaA"
     CDS             3932..5005
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid
                      pVS1 (Heeb et al., 2000)"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
                     /standard_name="pVS1 RepA"
     rep_origin      5071..5265
     primer_bind     complement(5501..5523)
                     /note="pGEX vectors, reverse primer"
                     /standard_name="pGEX 3'"
     misc_feature    5609..5749
                     /note="basis of mobility region from pBR322"
                     /standard_name="bom"
     primer_bind     5664..5683
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /standard_name="pRS-marker"
     primer_bind     complement(5764..5781)
                     /note="L4440 vector, forward primer"
                     /standard_name="L4440"
     rep_origin      complement(5935..6523)
     primer_bind     complement(6015..6034)
                     /note="pBR322 origin, forward primer"
                     /standard_name="pBR322ori-F"
     CDS             complement(6769..7560)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /standard_name="SmR"
     misc_feature    8081..8105
                     /note="left border repeat from nopaline C58 T-DNA"
                     /standard_name="LB T-DNA repeat"
     terminator      8198..8450
                     /note="nopaline synthase terminator and poly(A) signal"
                     /standard_name="NOS terminator"
     misc_feature    complement(8476..9024)
                     /note="Geneious type: ORF"
                     /standard_name="BAR ORF"
     promoter        complement(9066..9245)
                     /note="nopaline synthase promoter"
                     /standard_name="NOS promoter"
ORIGIN      
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttatggtgag caagggcgag gagctgttca
      481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      541 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      661 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      721 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      901 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      961 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1021 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     1081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1141 tcactctcgg catggacgag ctgtacaagt aatcgttcag ctttcttgta caaagtggtg
     1201 atatcccgcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     1261 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     1321 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1381 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     1441 atgcgacgtc gggcccaagc ttagcttgag cttggatcag attgtcgttt cccgccttca
     1501 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     1561 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     1621 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt
     1681 actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     1741 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     1801 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     1861 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     1921 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     1981 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     2041 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     2101 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     2161 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     2221 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     2281 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     2341 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     2401 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     2461 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     2521 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     2581 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     2641 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     2701 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     2761 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     2821 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     2881 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     2941 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3001 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     3061 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     3121 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     3181 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     3241 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     3301 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     3361 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     3421 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     3481 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     3541 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     3601 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     3661 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     3721 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     3781 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     3841 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     3901 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     3961 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     4021 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     4081 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     4141 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     4201 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     4261 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     4321 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     4381 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     4441 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     4501 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     4561 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     4621 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     4681 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     4741 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     4801 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     4861 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     4921 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     4981 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     5041 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     5101 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     5161 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     5221 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     5281 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     5341 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     5401 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     5461 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5581 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     5641 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     5701 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     5761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
     6601 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
     6661 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
     6721 agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac
     6781 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     6841 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     6901 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     6961 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     7021 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     7081 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     7141 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     7201 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     7261 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     7321 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     7381 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     7441 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     7501 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat
     7561 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     7621 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     7681 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     7741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
     7801 acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca ggcagcaacg
     7861 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
     7921 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
     7981 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
     8041 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
     8101 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa
     8161 ttaacgccga attgaattat cagcttgcat gccggtcgat ctagtaacat agatgacacc
     8221 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     8281 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     8341 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     8401 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc
     8461 taggggtcat cactagattt cggtgacggg caggaccgga cggggcggca ccggcaggct
     8521 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
     8581 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
     8641 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
     8701 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
     8761 ctcggccgtc cagtcgtagg cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc
     8821 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
     8881 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
     8941 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
     9001 ggccgggcgt cgttctgggc tcatggtaga tcccctcgat cgagttgaga gtgaatatga
     9061 gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa
     9121 atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac
     9181 gtatgtgctt agctcattaa actccagaaa cccgcggctc agtggctcct tcaacgttgc
     9241 ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg
     9301 tgactccctt aattctccgc tcatgatcga taattcgagc tctcccatat ggtcgactag
     9361 agccaagctg atctcctttg ccccggagat caccatggac gactttctct atctctacga
     9421 tctaggaaga aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa
     9481 gattagcctc ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc
     9541 ggcaggtctc atcaagacga tctacccgag taataatctc caggagatca aataccttcc
     9601 caagaaggtt aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa
     9661 agatatattt ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca
     9721 taaaccaagg caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa
     9781 ggccatggag tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga
     9841 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     9901 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     9961 ggctat
//
