LOCUS LZF1826_pAAV-hSy 7519 bp ds-DNA circular 26-OCT-2019 DEFINITION . ACCESSION urn.local...e-84v30fs (modified) VERSION urn.local...e-84v30fs KEYWORDS . SOURCE null ORGANISM . . COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers misc_feature 1..141 /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="L-ITR" promoter 156..640 /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="hSyn" primer_bind 601..620 /Sequence="AGCGGAGGAGTCGTGTCGTG" /Hairpin_Tm="None" /Tm="77.3" /Self_Dimer_Tm="None" /%GC="65.0" /created_by="primer3" /label="LZFsp22" primer_bind 626..645 /Sequence="GAGCGCAGTCGAGAAGGTAC" /Hairpin_Tm="38.1" /Tm="60.5" /Self_Dimer_Tm="11.5" /%GC="60.0" /created_by="primer3" /modified_by="kathrynevans" /label="KEE_003_F" misc_binding 647..652 /ApEinfo_fwdcolor="#ff4809" /ApEinfo_revcolor="#ff4809" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="BamHI" misc_feature 671..704 /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="Lox2272" misc_feature 755..788 /ApEinfo_label="LoxP" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="LoxP(1)" misc_feature 789..796 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="AscI" misc_feature 797..799 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature complement(800..994) /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="KV2.1TS(Codon Optimized)" primer_bind complement(994..1034) /created_by="kathrynevans" /Sequence="CATCAACGTGGTGTACCCATACGATGTTCCAGATTACGCAG" /modified_by="kathrynevans" /label="KEE_003_R" CDS complement(995..1021) /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="HA" primer_bind 1009..1046 /Sequence="CATCGTATGGGTACACCACGTTGATGTCGATCTGGTCC" /modified_by="kathrynevans" /label="KEE_002_F" motif complement(1022..1090) /created_by="kathrynevans" /modified_by="kathrynevans" /label="ts" source <1022..>1963 /organism="synthetic DNA construct" /mol_type="other DNA" gene complement(1091..1963) /created_by="kathrynevans" /label="GtACR2" primer_bind complement(1942..1978) /created_by="kathrynevans" /Sequence="GAAAACCCCGGTCCCATGGCATCACAGGTCGTCTATG" /modified_by="kathrynevans" /label="KEE_002_R" primer_bind 1948..1983 /created_by="kathrynevans" /Sequence="CGACCTGTGATGCCATGGGACCGGGGTTTTCTTCCA" /Hairpin_Tm="None" /Tm="95.7" /Self_Dimer_Tm="23.1" /%GC="58.3" /created_by="primer3" /modified_by="kathrynevans" /label="KEE_001_F" regulatory 1960..>1963 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)" CDS complement(1964..2029) /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="P2A" source complement(<2030..>3850) /organism="recombinant plasmid" /mol_type="other DNA" CDS complement(2030..2050) /codon_start=1 /note="ER2" /translation="FCYENEV" CDS complement(2051..2056) /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="YK" CDS complement(2057..2251) /codon_start=1 /note="Kv2.1" /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMR SMSSIDSFISCATDFPEATRF" CDS complement(2252..2269) /codon_start=1 /note="Linker" /translation="GGSGG" CDS complement(2270..2983) /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITLGMDELY" primer_bind complement(2312..2333) /Sequence="CATGGTCCTGCTGGAGTTCGTG" /Hairpin_Tm="44.2" /Tm="78.0" /Self_Dimer_Tm="13.4" /%GC="59.1" /created_by="primer3" /modified_by="kathrynevans" /label="EGFP-C-For" misc_feature 2324 /created_by="User" /modified_by="User" /label="mutate to T" misc_feature 2345..2361 /created_by="User" /label="YSP35" misc_feature 2723..2742 /created_by="User" /label="LZFsp03" CDS complement(2984..3043) /codon_start=1 /note="KGC" /translation="KSRITSEGEYIPLDQIDINV" CDS complement(3044..3091) /codon_start=1 /note="Linker" /translation="TGAADRPVVAVSKAAA" CDS complement(3092..3850) /codon_start=1 /note="Archon#22-45-TITI" /translation="MVSIALQAGYDLLGDGRPESLWLGIGTLLMLIGTFYFLVRAWGE TDKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVPVGGEMLNIYYARYAQWLFTTPL LLLHLALLAKVDRVTIGTLVGVDALMIVTGLIGTLSHTAIARYSWWLFSTICMIVVLY VLATSLRSAAKERGPEVASTFNILTALVLVLWTAYPIIWIIGTEGAGVVGLGIETLLF MVLDVTAKVGFGFILLRSRAILQDTEAPEPSAGAD" primer_bind complement(3105..3133) /Sequence="CTGCAAGATACAGAAGCACCTGAACCTTC" /Hairpin_Tm="37.3" /Tm="79.2" /Self_Dimer_Tm="11.6" /%GC="48.3" /created_by="primer3" /modified_by="kathrynevans" /label="KEE_001_R" primer_bind complement(3529..3550) /Sequence="CCACTGTTGCTGCTGCACCTGG" /Hairpin_Tm="50.3" /Tm="81.4" /Self_Dimer_Tm="18.7" /%GC="63.6" /created_by="primer3" /label="Mid-Archon_Rev" misc_binding 3860..3865 /ApEinfo_fwdcolor="#ff4809" /ApEinfo_revcolor="#ff4809" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="NheI" misc_feature 3866..3899 /ApEinfo_label="Lox2272" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="Lox2272(1)" misc_feature 3950..3983 /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="LoxP" misc_binding 3984..3989 /ApEinfo_fwdcolor="#ff4809" /ApEinfo_revcolor="#ff4809" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="EcoRI" misc_feature 4010..4597 /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="WPRE" primer_bind complement(4029..4055) /Sequence="AAGAATACCAGTCAATCTTTCACAAAT" /Hairpin_Tm="None" /Tm="70.5" /Self_Dimer_Tm="None" /%GC="29.6" /created_by="primer3" /label="LZFsp02" primer_bind complement(4560..4582) /Sequence="CAAAGGGAGATCCGACTCGTCTG" /Hairpin_Tm="41.5" /Tm="77.4" /Self_Dimer_Tm="None" /%GC="56.5" /created_by="primer3" /label="SP43" misc_feature complement(4636..4755) /vntifkey=21 /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="SV40_PA_terminator" misc_feature 4760..4765 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="PmlI" misc_feature 4782..4922 /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /modified_by="User" /label="R-ITR" rep_origin 5014..5320 /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="f1 Ori" misc_marker 5839..7519 /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="AmpR" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt 661 cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg 721 attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac 781 gaagttatgg cgcgccttag aatcgagtag cctctggaaa atcggtggcg catgaaatga 841 agctgtcgat actggacata gatcgcatgt ctataacgcc ttcagtccgt gctggaaggg 901 gtgcgacagg gctgggcatg gatgacatct ccaattcctc gggtggtttt gattgtgggg 961 ccatttcctt agtgttaagt ataggctgac tctgagcgta atctggaaca tcgtatgggt 1021 acaccacgtt gatgtcgatc tggtccaggg ggatgtactc gccctcgctg gtgatcctgc 1081 tcttggcggc ccttccaaac attctgatat tgacttcgtc gttaggaggg gcattcttct 1141 cgtgattcag gccaaaagga tcgtcggctt cgggtttctc ctgccctggc agacatgcgg 1201 ggtcccactt cccttccagc actccccagg cagtgctcca caggatcact ccatatgtat 1261 ttttgcacag gccgtcagcc agcaggtaca gaatggcgct cacgttctcg tggatcacgc 1321 agactccggt gggtgaaatc agccacacga ttgggaacat agtccaagca ataaagaaga 1381 tcaggcgcag catcttgatc ctcaccacca ccttctgtgc cagaggtgtc ccgacagatt 1441 caaagccata cagccccact tgaaagattg tgaagaccac actgtactcg aacaccaggc 1501 aggtggcccc aaagaaaaag aacagccact tcatatactc tgcgggagaa attgtagcgg 1561 tgattcccat gaccactcta atgatggaag cggcctgtgc aatgggattc agtgggatac 1621 ttttgtactt caccagagcc atgttagaaa tctgtcccag catgattgga caggtgcaca 1681 gccaggaggc cattcggctc caaggaaaga atctgccgtc cctcatctgc agggttccgt 1741 tgccagtact ggccagtgca taagtgatag attcgacaaa gggcaggtac acggactccc 1801 aagggacctt gggagccctg ctaatcatga agattgtctg gcagcctgag accacggccc 1861 acaccagctg cagcagtgag acgaaggtgg agtcgattct ggacatattg tagcactcgg 1921 tgtgagttga tgcccattcc ccatagacga cctgtgatgc catgggaccg gggttttctt 1981 ccacgtctcc tgcttgcttt aacagagaga agttcgtggc tccggagccc acctcgttct 2041 cgtagcagaa cttgtagaac ctggtggcct cagggaagtc ggtggcgcag ctgatgaagc 2101 tgtcgatgct gctcatgctc ctcatgtcga tcacgccctc ggtcctggca ggcagagggg 2161 ccacagggct aggcatgctg ctcatctcca gctcctcagg aggcttgctc tgaggggcca 2221 tctccttggt gttcaggata ggctggctct gggtacctcc gctcccaccc ttgtacagct 2281 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc 2341 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca 2401 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc 2461 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt 2521 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt 2581 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct 2641 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct 2701 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg 2761 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca 2821 cgggcagctt gccggtggtg caaatgaact tcagggtcag cttgccgtag gtggcatcgc 2881 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca 2941 ggatgggcac caccccggtg aacagctcct cgcccttgct caccacgttg atgtcgatct 3001 ggtccagggg gatgtactcg ccctcgctgg tgatcctgct cttggcggcc gccttgctca 3061 ctgctactac cgggcggtcg gcggcaccgg tatcagctcc agctgaaggt tcaggtgctt 3121 ctgtatcttg cagaatggct cttgacctga gcaggataaa accgaagccc accttggcgg 3181 tgacgtccag caccatgaac agcaaagttt cgattcccag gcccacaact ccggcaccct 3241 cggtgccgat aatccagatg atagggtaag cagtccacag gaccagaacc agtgcggtga 3301 ggatgttgaa ggttgaggca acctctgggc ctctctcctt agcggctgac cgcagagaag 3361 tggccaggac atacagaacg acgatcatac agatagttga gaacagccac caagagtatc 3421 tggcaatggc agtatgtgac agtgttccta tcaggcctgt gacaatcatc agagcatcga 3481 cgccaacgag agtgccgatg gtcactctat caactttggc caggagtgcc aggtgcagca 3541 gcaacagtgg tgtagtgaac agccactgtg cgtatcttgc gtagtagatg ttcagcatct 3601 ctccacctac tggcacctct gtgaggccaa taccaaagaa cattgacaga taagctgcac 3661 tggcaatacc gctgaccagg attgtcactg cgtagtactc ccgtgcatcc ttgtcagtct 3721 ctccccacgc tctaaccagg aagtagaagg ttccgatcag catcagcagt gtaccaattc 3781 cgagccacag actttcaggt ctaccatctc cgaggaggtc ataaccagcc tgcagtgcga 3841 tagaaaccat ggtggcggag ctagcataac ttcgtataaa gtatcctata cgaagttatt 3901 tgccttaacc cagaaattat cactgttatt ctttagaatg gtgcaaagaa taacttcgta 3961 taatgtatgc tatacgaagt tatgaattcg atatcaagct tatcgataat caacctctgg 4021 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 4081 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 4141 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 4201 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 4261 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 4321 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 4381 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca 4441 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 4501 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 4561 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cccgggcggc 4621 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 4681 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 4741 taagctgcaa taaactaacc acgtgcggac cgagcggccg caggaacccc tagtgatgga 4801 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 4861 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca 4921 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg 4981 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 5041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 5101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 5161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat 5221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 5281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc 5341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 5401 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc 5461 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca 5521 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 5581 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 5641 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 5701 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 5761 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 5821 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 5881 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 5941 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 6001 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 6061 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 6121 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 6181 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 6241 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 6301 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 6361 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 6421 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 6481 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 6541 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 6601 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 6661 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 6721 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6781 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6841 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 6901 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 6961 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 7021 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 7081 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 7141 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 7201 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 7261 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 7321 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 7381 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 7441 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 7501 tggccttttg ctcacatgt //