LOCUS       Exported                8846 bp ds-DNA     circular SYN 29-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 1, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(67..582)
                     /label=CEN6_ARSH4
     misc_feature    610..713
                     /label=Amp promoter
     misc_feature    714..1571
                     /label=Ampicillin
     misc_feature    complement(1572..1574)
                     /label=Ampicillin
     misc_feature    1572..1574
                     /label=Stop codon
     misc_feature    1745..2334
                     /label=Ori
     misc_feature    complement(2757..2776)
                     /label=T3
     misc_feature    2793..3432
                     /label=Gal 1 Promoter
     misc_feature    3463..4197
                     /label=mcherry
     misc_feature    4198..4278
                     /label=linker
     misc_feature    4279..5775
                     /label=CRY2PHR hoc
     misc_feature    5776..5844
                     /label=linker
     misc_feature    join(5845..6479,6481..6501)
                     /label=TEV full length 242
     misc_feature    6301..6303
                     /label=S153N
     misc_feature    6480
                     /label=New Feature
     misc_feature    6499..6501
                     /label=S 219
     misc_feature    6502..6507
                     /label=Stop codon
                     /label=Stop codon(1)
     terminator      6514..6765
                     /label=CYC1_terminator
                     /note="CYC1_terminator"
                     /note="26to274"
     misc_feature    6965..7416
                     /label=Ori F1
     misc_feature    complement(7683..8342)
                     /label=HIS3
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     2761 accctcacta aagggaacaa aagctggagc tccgcaaaga agtttaataa tcatattaca
     2821 tggcattacc accatataca tatccatata catatccata tctaatctta cttatatgtt
     2881 gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca
     2941 gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt
     3001 gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct
     3061 gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc
     3121 ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa
     3181 cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg
     3241 gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact
     3301 gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat
     3361 gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa
     3421 aaaaccccgg atcgaattct acttcataca ttttcaatta agatggctcg ggatccaccg
     3481 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3541 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3601 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3661 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3721 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3781 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3841 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3901 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     3961 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     4021 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     4081 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     4141 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggct
     4201 ctgggaggtc aaggtctgca ggctagtggt ggaggaggct ctggtggagg cggtagcgga
     4261 ggcggagggt cggctagcat gaagatggac aaaaagacga ttgtgtggtt tagacgtgac
     4321 cttcgcattg aggacaaccc tgcgttggca gcggcagcgc atgaaggttc ggtattccct
     4381 gtctttatct ggtgtcccga agaggagggg caattctatc ccggtcgcgc gtcccgatgg
     4441 tggatgaaac agtcgctcgc acatcttagc cagtcgttga aggcgctcgg ctctgatctg
     4501 acgctcatca aaacacacaa tacaatttct gccattctgg attgcattag ggtgactggt
     4561 gccaccaaag ttgtgttcaa tcacctttat gatcccgtaa gcctggtcag ggatcataca
     4621 gtaaaagaga aacttgtaga gaggggaatc agcgtccaat cgtacaatgg ggacttgttg
     4681 tatgaaccgt gggaaatcta ttgtgagaaa ggcaaacctt tcacttcctt caattcgtac
     4741 tggaagaaat gcctcgacat gtccatcgag tcagtcatgc tgccgccacc ttggagactt
     4801 atgcccatta ctgctgcggc agaggcgatc tgggcttgca gcatcgaaga acttggtctg
     4861 gaaaacgaag cggaaaaacc cagtaacgcg ctcctgacgc gagcgtggtc gccaggctgg
     4921 tcgaatgctg ataagctcct taatgagttt atcgagaaac agttgatcga ctatgcgaag
     4981 aatagcaaga aggtggtggg aaattccact tccctcctgt cgccgtatct ccactttggg
     5041 gagattagcg tgaggcatgt gttccaatgt gcgcggatga agcaaatcat ttgggctcgc
     5101 gacaaaaact ctgaaggaga ggagtctgcc gatttgtttt tgagaggaat cggccttagg
     5161 gagtattcac gatacatctg cttcaacttt ccgtttacac atgaacaatc cctcctcagc
     5221 cacctccgct tctttccgtg ggacgcggac gtggacaaat tcaaggcctg gcggcagggg
     5281 cgcacgggat acccgttggt ggatgcgggt atgagagagc tgtgggcaac gggttggatg
     5341 cacaaccgta ttcgagtcat cgttagtagc tttgccgtta agttcctctt gttgccctgg
     5401 aaatggggga tgaaatactt ttgggacacg cttctggatg cggacttgga gtgcgatatt
     5461 cttgggtggc agtacatttc gggttccatc cctgacgggc atgaacttga ccgattggat
     5521 aaccctgcac tgcaaggagc aaagtacgac ccggagggag aatacatcag gcagtggctc
     5581 ccagagctgg cccgtctgcc caccgagtgg attcaccacc cgtgggatgc tcccctgacc
     5641 gtccttaaag cctcaggggt cgaattgggg acgaattacg cgaaacccat cgtcgatatt
     5701 gacaccgcac gagaattgct ggcgaaagcc atctcaagaa cacgcgaagc gcagattatg
     5761 atcggagcag cgccgggagg tagcggctct ggtggaagtg gatcaggcag cggtggatct
     5821 ggcagcggaa agcttggttc cgggggagaa tccttgttta agggaccacg tgattacaac
     5881 ccgatatcga gcaccatttg tcatttgacg aatgaatctg atgggcacac aacatcgttg
     5941 tatggtattg gatttggtcc cttcatcatt acaaacaagc acttgtttag aagaaataat
     6001 ggaacactgt tggtccaatc actacatggt gtattcaagg tcaagaacac cacgactttg
     6061 caacaacacc tcattgatgg gagggacatg ataattattc gcatgcctaa ggatttccca
     6121 ccatttcctc aaaagctgaa atttagagag ccacaaaggg aagagcgcat atgtcttgtg
     6181 acaaccaact tccaaactaa gagcatgtct agcatggtgt cagacactag ttgcacattc
     6241 ccttcatctg atggcatatt ctggaagcat tggattcaaa ccaaggatgg gcagtgtggc
     6301 aatccattag tatcaactag agatgggttc attgttggta tacactcagc atcgaatttc
     6361 accaacacaa acaattattt cacaagcgtg ccgaaaaact tcatggaatt gttgacaaat
     6421 caggaggcgc agcagtgggt tagtggttgg cgattaaatg ctgactcagt attgtggggg
     6481 ggccataaag ttttcatggt gtaatagctc gagtcatgta attagttatg tcacgcttac
     6541 attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag
     6601 tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     6661 aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct
     6721 tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac ccaattcgcc
     6781 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     6841 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6901 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6961 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7021 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7081 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7141 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7201 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7261 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7321 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7381 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     7441 ttacgcatct gtgcggtatt tcacaccgca tagatccgtc gagttcaaga gaaaaaaaaa
     7501 gaaaaagcaa aaagaaaaaa ggaaagcgcg cctcgttcag aatgacacgt atagaatgat
     7561 gcattacctt gtcatcttca gtatcatact gttcgtatac atacttactg acattcatag
     7621 gtatacatat atacacatgt atatatatcg tatgctgcaa ctttaaataa tcggtgtcac
     7681 tacataagaa cacctttggt ggagggaaca tcgtttgtac cattgggcga ggtggcttct
     7741 cttatggcaa ccgcaagagc cttgaacgca ctctcactac ggtgatgatc attcttgcct
     7801 cgcagacaat caacgtggag ggtaattctg ctagcctctg caaaactttc aagaaaatgc
     7861 gggatcatct cgcaagagag atctcctact ttctcccttt gcaaaccaag ttcgacaact
     7921 gcgtacggcc tgttcgaaag atctaccacc gctctggaaa gtgcctcatc caaaggcgca
     7981 aatcctgatc caaacctttt tactccacgc acggccccta gggcctcttt aaatgcttga
     8041 ccgagagcaa tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa gtcaccaatg
     8101 cactcaacga ttagcgacca gccggaatgc ttggccagag catgtatcat atggtccaga
     8161 aaccctatac ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct
     8221 tctgcctctt tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag
     8281 atcgcaatct gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc
     8341 atctttgcct tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca
     8401 ttactttata taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga
     8461 gtcatccgct aggtggaaaa aaaaaaatga aaatcattac cgaggcataa aaaaatatag
     8521 agtgtactag aggaggccaa gagtaataga aaaagaaaat tgcgggaaag gactgtgtta
     8581 tgacttccct gactaatgcc gtgttcaaac gatacctggc agtgactcct agcgctcacc
     8641 aagctcttaa aacgggaatt tatggtgcac tctcagtaca atctgctctg atgccgcata
     8701 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8761 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8821 ttcaccgtca tcaccgaaac gcgcga
//
