LOCUS       Exported                7357 bp ds-DNA     circular SYN 29-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 1, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    72..1234
                     /label=lacI
     misc_feature    1407..1435
                     /label=tac-promoter
     misc_feature    1447..1469
                     /label=M13_pUC_rev_primer
     misc_feature    1528..3408
                     /label=MBP-TEVRs-TEV-R-His6
     misc_feature    2602..2625
                     /label=MBP_F_primer
     misc_feature    2692..2712
                     /label=TEV cleavable site ENLYFQG
     misc_feature    2713..2733
                     /label=His6
     misc_feature    2821..2823
                     /label=T30
     misc_feature    3190..3192
                     /label=S153
     misc_feature    3388..3390
                     /label=V219
     misc_feature    3406..3411
                     /label=Stop codon
     misc_feature    3456..3599
                     /label=lacZ_a
     misc_feature    4059..4087
                     /label=Amp Promoter
     misc_feature    4129..4989
                     /label=Ampicillin
     misc_feature    5221..5527
                     /label=f1_origin
     misc_feature    5639..6258
                     /label=pBR3222_origin
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagattatg aaaactgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaagggg agaaaatctt
     2701 tattttcaag gtcatcatca tcatcatcat catggagaaa gcttgtttaa ggggccgcgt
     2761 gattacaacc cgatatcgag caccatttgt catttgacga atgaatctga tgggcacaca
     2821 acatcgttgt atggtattgg atttggtccc ttcatcatta caaacaagca cttgtttaga
     2881 agaaataatg gaacactgtt ggtccaatca ctacatggtg tattcaaggt caagaacacc
     2941 acgactttgc aacaacacct cattgatggg agggacatga taattattcg catgcctaag
     3001 gatttcccac catttcctca aaagctgaaa tttagagagc cacaaaggga agagcgcata
     3061 tgtcttgtga caaccaactt ccaaactaag agcatgtcta gcatggtgtc agacactagt
     3121 tgcacattcc cttcatctga tggcatattc tggaagcatt ggattcaaac caaggatggg
     3181 cagtgtggca gtccattagt atcaactaga gatgggttca ttgttggtat acactcagca
     3241 tcgaatttca ccaacacaaa caattatttc acaagcgtgc cgaaaaactt catggaattg
     3301 ttgacaaatc aggaggcgca gcagtgggtt agtggttggc gattaaatgc tgactcagta
     3361 ttgtgggggg gccataaagt tttcatggtg cgtcgtcgcc gtcgctaata aggatcctct
     3421 agagtcgacc tgcaggcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     3481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3601 tggcagcttg gctgttttgg cggatgagat aagattttca gcctgataca gattaaatca
     3661 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
     3721 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
     3781 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     3841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     3901 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     3961 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     4021 gtttctacaa actctttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4141 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4201 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4261 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgttct
     4321 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4381 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4441 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4501 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4561 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4621 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4681 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4741 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4801 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4861 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4921 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4981 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt accccggttg
     5041 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg
     5101 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5161 aggccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg
     5221 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5281 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt
     5341 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5401 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5461 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5521 ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc
     5581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5641 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5701 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5761 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     5821 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5881 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5941 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6001 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6061 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6121 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6181 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6241 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6301 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6361 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6421 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     6481 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     6541 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     6721 gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     6781 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6841 cggttttttc ctgtttggtc acttgatgcc tccgtgtaag ggggaatttc tgttcatggg
     6901 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     6961 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     7021 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     7081 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     7141 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
     7201 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     7261 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     7321 gcgcacccgt ggccaggacc caacgctgcc cgaaatt
//
