LOCUS       Exported                5296 bp ds-DNA     circular SYN 30-OCT-2019
DEFINITION  Ligation of luc into 3M-5M-Ndei-NotI.
ACCESSION   .
VERSION     .
KEYWORDS    3M-5M-Fluc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 5.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..1654
                     /label='luciferase gene
                     /note="ORF luc"
     misc_feature    1663..2100
                     /label=MBGV trailer
                     /note="MBGV trailer"
     misc_feature    complement(2099..2117)
                     /label=T7 promoter
                     /note="T7 RNA polymerase promoter"
     primer_bind     complement(2229..2249)
                     /label=Primer #339
                     /note="from 3M-5M-luc"
     misc_feature    3135..3995
                     /label=AmpR
                     /note="ß-lactamase (AmpR) coding region"
     primer_bind     5007..5030
                     /label=Primer #340
                     /note="from 3M-5M-luc"
     misc_feature    complement(5095..5179)
                     /label=HDV core ribozyme
                     /note="hepatitis delta virus core ribozyme"
     misc_feature    5180..5285
                     /label=MBGV leader
                     /note="MBGV leader"
ORIGIN
        1 tatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
       61 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      121 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      181 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      241 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      301 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      361 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      421 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
      481 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      541 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
      601 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
      661 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
      721 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
      781 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
      841 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
      901 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
      961 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     1021 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     1081 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     1141 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     1201 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     1261 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     1321 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     1381 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     1441 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     1501 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     1561 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     1621 aaaggccaag aagggcggaa agatcgccgt gtaagcggcc gctagtgatt agtgtttggt
     1681 cacaccagag gtaacttttt aacgttaatt actcagatgt tattgctcat aattagcatt
     1741 aatattggca cattgggtga atccttgagc tttatcccta atatggtgta agaaattaag
     1801 gaaatactga gatacactag ttgaattgaa ttatgacata ccatatatca taaatataaa
     1861 aaagtgtctg ctgtaatcta caagcacctc ttttaaatac attaggaaaa gaattaagtt
     1921 accgttgaga tcaaaaaaac cacgtcatgt tttctctgat gacaagtgat aaaacttcgt
     1981 agttaaattt ctagaatgtc gatgtgaatg taaattaaga aaaaccaata tataaaatta
     2041 aaaaattaaa aagctttgat ataagtaaca caaaacattc ttcatctttt ttgtgtgtcc
     2101 tatagtgagt cgtattaatt tcgcgggatc gatatccccc tgcattaatg aatcggccaa
     2161 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2221 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2281 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2341 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2401 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2461 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2521 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2581 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2641 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2701 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2761 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2821 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2881 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2941 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3001 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3061 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3121 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3181 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3241 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3301 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3361 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3421 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3481 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3541 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3601 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3661 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3721 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3781 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3841 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3901 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3961 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4021 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4081 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4141 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     4201 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4321 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     4381 atcgacgctc tcccttatgc gactcctgca ttaagaagca gcccagtagt aggttgaggc
     4441 cgttgagcac cgccgccgca aggaatggtg caaggagatg gcgcccaaca gtcccccggc
     4501 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     4561 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     4621 ggtgatgccg gccacgatgc gtccggcgta gaggatctgg ctagcgatga ccctgctgat
     4681 tggttcgctg accatttccg gggtgcggaa cggcgttacc agaaactcag aaggttcgtc
     4741 caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc
     4801 cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca
     4861 taaccttatg tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcatgc
     4921 ctgcaggtcg actctagagg atctcgatcc ggatatagtt cctcctttca gcaaaaaacc
     4981 cctcaagacc cgtttagagg ccccaagggg ttatgctagt tattgctcag cggtggcagc
     5041 agccaactca gcttcctttc gggctttgtt agcagccgga tccttttttt tgagctctcc
     5101 cttagccatc cgagtggacg acgtcctcct tcggatgccc aggtcggacc gcgaggaggt
     5161 ggagatgcca tgccgaccca gacacacaaa aacaagagat gatgattttg tgtatcatat
     5221 aaataaagaa gaatattaac attgacattg agacttgtca gtctgttaat attcttgaag
     5281 agatgttttt atctca
//
