LOCUS       Exported               12734 bp ds-DNA     circular SYN 02-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(686..1315)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     protein_bind    complement(3432..3479)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        4080..4295
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4296..5099
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    5264..5288
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5480..5532
                     /label=AdhHygF
     primer_bind     5484..5500
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5510..5697
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5700..5716
                     /label=LifeBlock_R
     CDS             complement(5719..6747)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(6007..6026)
                     /label=HygRSeqDown
     primer_bind     complement(6727..6767)
                     /label=UTRHygR
     primer_bind     6735..6773
                     /label=H2B2D_F
     primer_bind     6735..6758
                     /label=LifeBlock_F
     CDS             complement(6756..6873)
                     /codon_start=1
                     /label=5UTR
                     /translation="GDAPRHSPKSFSVFCYASSFDVSLQDSLTTLSLTHTLHS"
     misc_feature    6874..7090
                     /label=H2B2D
     primer_bind     6875..6894
                     /label=H2BprF1
     primer_bind     7020..7039
                     /label=H2BprF2
     primer_bind     7047..7065
                     /label=TwistLifeact_F
     CDS             7091..7156
                     /codon_start=1
                     /label=5UTR
                     /translation="SQTLCDLTKTFHPHHSHPSHTQ"
     misc_feature    7103..7334
                     /label=twist_22C-1
     misc_feature    7129..7472
                     /label=twist_22C-2
     misc_feature    7129..7427
                     /label=twist_22C-3
     primer_bind     7134..7154
                     /label=minLife_F
     primer_bind     complement(7137..7227)
                     /label=LAH2Bpr_R
     primer_bind     7158..7246
                     /label=LATdTom_F
     CDS             7163..7213
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             7229..8659
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(7232..7246)
                     /label=mLife_R
     primer_bind     complement(7251..7270)
                     /label=TwistLifeact_R
     primer_bind     complement(7977..7996)
                     /label=TwistLifeact_R
     primer_bind     complement(8624..8642)
                     /label=TomR2
     primer_bind     complement(8637..8678)
                     /label=TdLife_R
     primer_bind     complement(8637..8656)
                     /label=TomR1
     primer_bind     complement(8662..8678)
                     /label=LifeBlock_R
     terminator      8681..8868
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8846..8868)
                     /label=AdhHygF
     primer_bind     complement(8883..8899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8907..8923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8931..8961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8976..8997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    9174..9198
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9491..10306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      10401..10989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    11175..11315
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      11659..11853
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(11919..12734,1..252))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
       61 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
      121 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
      181 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
      241 tggtttgcga ccgtcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
      301 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
      361 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
      421 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
      481 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
      541 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
      601 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
      661 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
      721 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
      781 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
      841 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
      901 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
      961 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
     1021 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
     1081 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
     1141 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
     1201 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
     1261 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
     1321 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
     1381 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
     1441 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
     1501 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
     1561 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
     1621 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
     1681 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
     1741 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
     1801 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
     1861 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
     1921 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
     1981 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
     2041 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
     2101 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
     2161 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
     2221 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
     2281 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
     2341 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
     2401 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
     2461 gaaaagtgcc acctgcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc
     2521 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag
     2581 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag
     2641 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     2701 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc
     2761 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct
     2821 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc
     2881 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact
     2941 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct
     3001 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca
     3061 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa
     3121 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt
     3181 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa
     3241 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag
     3301 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg
     3361 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc
     3421 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca
     3481 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     3541 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     3601 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa
     3661 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt
     3721 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct
     3781 tcaatgctat catttccttt gatattggat catactaaga aaccattatt atcatgacat
     3841 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     3901 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     3961 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     4021 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataccaca gcttttcaat
     4081 tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga aatttttttg
     4141 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
     4201 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
     4261 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
     4321 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
     4381 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
     4441 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
     4501 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
     4561 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
     4621 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
     4681 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
     4741 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
     4801 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
     4861 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
     4921 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
     4981 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
     5041 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
     5101 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
     5161 tatatcagtt attaccggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
     5221 cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc cgccaatata
     5281 tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca
     5341 agctcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     5401 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     5461 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttc cggtagaggt
     5521 gtggtcaata agagcgacct catactatac ctgagaaagc aacctgacct acaggaaaga
     5581 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
     5641 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
     5701 tttagaagtg gcgcgccatt attcctttgc cctcggacga gtgctggggc gtcggtttcc
     5761 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     5821 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     5881 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     5941 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     6001 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     6061 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     6121 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     6181 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     6241 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     6301 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     6361 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     6421 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     6481 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     6541 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     6601 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     6661 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     6721 cgcggtgagt tcaggctttt tacccattta attaatgctg tgtaaggtgt gcgtgagaga
     6781 gagggtcgtg agtgagtctt gaagagaaac gtcaaagctt gaagcgtagc agaatacgga
     6841 aaacgacttg ggggaatggc gaggagcgtc gccttttatg ctccaagcgg agacgatgag
     6901 gccgatatta cagtggctta gtagcgtgta caatatttcc ataaaaggct acatggtgag
     6961 tttttcacgt gaccgtaatt agtcccaatt aggctttatt gtatattgct actaagcatc
     7021 gttaaatgac ctgctcgaag cttcgtctat aaaaggcggg cgtgtgtgcg cttcccccgc
     7081 tacgtctttt tcacaaacac tttgcgactt gaccaagacc tttcacccgc accactctca
     7141 tccatcacac acacaagtcg acatgggcgt ggccgacttg atcaagaagt tcgagtccat
     7201 ctccaaggag gagggaggag gaggatccat ggtgagcaag ggcgaggagg tcatcaaaga
     7261 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     7321 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     7381 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     7441 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     7501 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     7561 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     7621 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     7681 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     7741 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     7801 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     7861 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg
     7921 cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat
     7981 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     8041 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     8101 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     8161 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     8221 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     8281 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     8341 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     8401 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     8461 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     8521 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     8581 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     8641 ggacgagctg tacaagtaaa ggcgcgccac ttctaaataa gcgaatttct tatgatttat
     8701 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     8761 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     8821 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggga attcgtaatc
     8881 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8941 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9001 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9061 atcggccaac gcgcggggag aggcggtttg cgtattggct agagcaattc ggcgttaatt
     9121 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     9181 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     9241 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     9301 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     9361 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     9421 ttgctgcctg tgatccagat catgaacaat aaaactgtct gcttacataa acagtaatac
     9481 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc
     9541 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     9601 tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     9661 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     9721 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     9781 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     9841 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     9901 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     9961 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
    10021 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
    10081 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
    10141 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
    10201 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
    10261 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaagaat taattcatga
    10321 tcctgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    11041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11101 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    11161 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
    11221 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
    11281 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
    11341 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    11401 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg
    11461 ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga
    11521 ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa
    11581 gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct
    11641 gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt
    11701 aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt
    11761 gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac
    11821 ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc
    11881 cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg
    11941 ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc
    12001 ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac
    12061 ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct
    12121 tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg
    12181 atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg
    12241 cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt
    12301 acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc
    12361 ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct
    12421 accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca
    12481 acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg
    12541 gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg
    12601 ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg
    12661 ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta
    12721 tcgcgggtgc ccac
//
