LOCUS       Exported               11629 bp ds-DNA     circular SYN 04-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1092..2428
                     /label=2u Ori
     protein_bind    complement(2049..2096)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2697..2912
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2913..3716
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    3881..3905
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4101..4117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4124..4408
                     /label=SpH2B
     CDS             4416..5441
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     5429..5474
                     /label=EM18Clover3_F
     CDS             5457..6173
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(6154..6201)
                     /label=EM18Clover3_R
     terminator      6193..6380
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6395..6411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6419..6435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6443..6473)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6488..6509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6686..6710
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7003..7818
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7913..8501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8687..8827
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      9171..9365
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9431..10498)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10932..11561)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
       61 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
      121 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
      181 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
      241 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
      301 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
      361 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
      421 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
      481 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
      541 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
      601 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
      661 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
      721 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
      781 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
      841 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
      901 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
      961 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
     1021 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaaggaa
     1081 aagtgccacc tgcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     1141 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     1201 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     1261 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     1321 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     1381 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     1441 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     1501 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     1561 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     1621 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     1681 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     1741 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     1801 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     1861 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     1921 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     1981 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     2041 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     2101 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     2161 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     2221 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     2281 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     2341 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     2401 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     2461 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2641 actatgcggc atcagagcag attgtactga gagtgcacca taccacagct tttcaattca
     2701 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     2761 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     2821 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     2881 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     2941 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     3001 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     3061 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     3121 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     3181 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     3241 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     3301 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     3361 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     3421 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     3481 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     3541 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     3601 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     3661 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     3721 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     3781 atcagttatt accggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
     3841 ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
     3901 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
     3961 tcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4021 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4081 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttttat gctccaagcg
     4141 gagacgatga ggccgatatt acagtggctt agtagcgtgt acaatatttc cataaaaggc
     4201 tacatggtga gtttttcacg tgaccgtaat tagtcccaat taggctttat tgtatattgc
     4261 tactaagcat cgttaaatga cctgctcgaa gcttcgtcta taaaaggcgg gcgtgtgtgc
     4321 gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact tgaccaagac ctttcacccg
     4381 caccactctc atccatcaca cacacaaatt aattaatggg taaaaagcct gaactcaccg
     4441 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     4501 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     4561 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     4621 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     4681 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     4741 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     4801 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     4861 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     4921 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     4981 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     5041 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     5101 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     5161 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     5221 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     5281 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     5341 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     5401 gtggaaaccg acgccccagc actcgtccga gggcaaagga aggaggagga ggatccatgg
     5461 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5521 acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga gggcgatgcc accaacggca
     5581 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5641 tgaccacctt cggctacggc gtggcctgct tcagccgcta ccccgaccac atgaagcagc
     5701 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctctttca
     5761 aggacgacgg tacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5821 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5881 tggagtacaa cttcaacagc cactacgtct atatcacggc cgacaagcag aagaactgca
     5941 tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagcgtgcag ctcgccgacc
     6001 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6061 tgagccatca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6121 tggagttcgt gaccgccgcc gggattacac atggcatgga cgagctgtac aaggcgcgcc
     6181 acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa
     6241 aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc
     6301 ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt
     6361 attgaccaca cctctaccgg gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     6421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6601 tgcgtattgg ctagagcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     6661 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     6721 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     6781 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     6841 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     6901 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatccag atcatgaaca
     6961 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     7021 gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     7081 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     7141 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     7201 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     7261 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
     7321 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     7381 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     7441 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     7501 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     7561 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     7621 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     7681 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     7741 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     7801 ctcgatgagt ttttctaaga attaattcat gatcctgcat gaccaaaatc ccttaacgtg
     7861 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7921 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7981 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8041 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8101 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8161 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8221 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8281 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8341 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8401 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8461 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8521 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8581 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8641 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8701 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8761 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8821 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8881 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8941 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     9001 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     9061 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     9121 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     9181 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     9241 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     9301 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     9361 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     9421 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     9481 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     9541 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     9601 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     9661 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     9721 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9781 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9841 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9901 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     9961 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    10021 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    10081 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    10141 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    10201 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    10261 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    10321 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    10381 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    10441 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgt
    10501 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
    10561 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
    10621 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
    10681 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
    10741 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
    10801 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
    10861 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
    10921 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
    10981 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
    11041 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
    11101 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
    11161 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
    11221 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
    11281 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
    11341 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
    11401 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
    11461 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
    11521 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
    11581 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagct
//
