LOCUS       Exported               11633 bp ds-DNA     circular SYN 30-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 30, 2019 from SnapGene Viewer 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1092..2428
                     /label=2micron
     protein_bind    complement(2049..2096)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2697..2912
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2913..3716
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    3881..3905
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4101..4117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4124..4408
                     /label=SpH2Bpr
     primer_bind     4271..4290
                     /label=H2BprF2
     CDS             4416..5441
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     5427..5474
                     /label=EM18Cerulean_F
     CDS             5457..6176
                     /codon_start=1
                     /label=mCerulean3
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6154..6207)
                     /label=CeruleanADHR
     primer_bind     complement(6155..6207)
                     /label=EM18Cerulean_R
     terminator      6197..6384
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6399..6415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6423..6439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6447..6477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6492..6513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6690..6714
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7007..7822
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7917..8505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8691..8831
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      9175..9369
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9435..10502)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10936..11565)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
       61 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
      121 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
      181 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
      241 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
      301 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
      361 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
      421 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
      481 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
      541 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
      601 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
      661 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
      721 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
      781 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
      841 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
      901 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
      961 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
     1021 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaaggaa
     1081 aagtgccacc tgcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     1141 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     1201 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     1261 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     1321 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     1381 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     1441 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     1501 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     1561 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     1621 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     1681 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     1741 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     1801 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     1861 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     1921 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     1981 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     2041 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     2101 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     2161 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     2221 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     2281 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     2341 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     2401 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     2461 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     2521 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     2581 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     2641 actatgcggc atcagagcag attgtactga gagtgcacca taccacagct tttcaattca
     2701 attcatcatt ttttttttat tctttttttt gatttcggtt tctttgaaat ttttttgatt
     2761 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     2821 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     2881 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     2941 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     3001 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     3061 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     3121 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     3181 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     3241 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     3301 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     3361 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     3421 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     3481 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     3541 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     3601 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     3661 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     3721 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     3781 atcagttatt accggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
     3841 ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
     3901 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
     3961 tcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4021 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4081 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttttat gctccaagcg
     4141 gagacgatga ggccgatatt acagtggctt agtagcgtgt acaatatttc cataaaaggc
     4201 tacatggtga gtttttcacg tgaccgtaat tagtcccaat taggctttat tgtatattgc
     4261 tactaagcat cgttaaatga cctgctcgaa gcttcgtcta taaaaggcgg gcgtgtgtgc
     4321 gcttcccccg ctacgtcttt ttcacaaaca ctttgcgact tgaccaagac ctttcacccg
     4381 caccactctc atccatcaca cacacaaatt aattaatggg taaaaagcct gaactcaccg
     4441 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     4501 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     4561 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     4621 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     4681 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     4741 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     4801 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     4861 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     4921 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     4981 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     5041 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     5101 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     5161 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     5221 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     5281 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     5341 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     5401 gtggaaaccg acgccccagc actcgtccga gggcaaagga aggaggagga ggatccatgg
     5461 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5521 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5581 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5641 tgaccaccct gagctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5701 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5761 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5821 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5881 tggagtacaa cgccatccac ggcaacgtct atatcaccgc cgacaagcag aagaacggca
     5941 tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6001 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6061 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     6121 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggcg
     6181 cgccacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat
     6241 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     6301 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt atgaggtcgc
     6361 tcttattgac cacacctcta ccgggaattc gtaatcatgt catagctgtt tcctgtgtga
     6421 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6481 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6541 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6601 ggtttgcgta ttggctagag caattcggcg ttaattcagt acattaaaaa cgtccgcaat
     6661 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     6721 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     6781 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     6841 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     6901 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat ccagatcatg
     6961 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     7021 acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg
     7081 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     7141 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     7201 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     7261 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     7321 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     7381 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     7441 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     7501 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     7561 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     7621 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     7681 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     7741 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     7801 gatgctcgat gagtttttct aagaattaat tcatgatcct gcatgaccaa aatcccttaa
     7861 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7921 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7981 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8041 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     8101 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8161 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8221 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8281 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8341 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8401 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8461 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8521 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8581 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8641 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     8701 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8761 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8821 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8941 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     9001 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     9061 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     9121 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     9181 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     9241 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     9301 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     9361 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     9421 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     9481 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9541 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     9601 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     9661 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     9721 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9781 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9841 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9901 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9961 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
    10021 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    10081 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    10141 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    10201 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    10261 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    10321 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    10381 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    10441 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    10501 ccgtcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac
    10561 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    10621 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    10681 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    10741 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    10801 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    10861 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    10921 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    10981 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    11041 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    11101 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    11161 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    11221 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    11281 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    11341 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    11401 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    11461 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    11521 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    11581 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gct
//
