LOCUS       pXT130_TwinStrepSUMO_ShTnsC        6911 bp ds-DNA     circular     30-OCT-2019
DEFINITION  .
ACCESSION   urn.local...5a3-b4z8wpq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_binding    497..519
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="LacO"
     misc_feature    568..717
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="6x His tag/ Twin Strep"
     misc_feature    718..1008
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="Sumo"
     Tn7             1015..1878
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /modified_by="Jonathan Strecker"
                     /label="TnsC"
     CDS             2569..3228
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="AmpR"
     rep_origin      3326..4008
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="ColE1 origin"
ORIGIN      
        1 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
       61 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
      121 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
      181 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
      241 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
      301 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
      361 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
      421 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
      481 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      541 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
      601 ggcctggtgc cgcgcggcag ccatatggct agctggagcc atccgcagtt tgaaaaaggt
      661 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaaatg
      721 tcggactcag aagtcaatca agaagctaag ccagaggtca agccagaagt caagcctgag
      781 actcacatca atttaaaggt gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag
      841 accactcctt taagaaggct gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac
      901 tccttaagat tcttgtacga cggtattaga atccaagctg atcagacccc tgaagatttg
      961 gacatggagg ataacgatat tattgaggct cacagagaac agattggtgg atccacagag
     1021 gcccaggcca ttgccaaaca gctcggcgga gtgaagcccg acgatgaatg gctgcaggcc
     1081 gagatcgcta gactgaaggg caagagcatc gtgcccctgc agcaagtgaa aaccctgcac
     1141 gattggctgg acggcaagcg gaaggccaga aagtcctgta gagtcgtggg cgagagcaga
     1201 accggaaaga ccgtggcctg tgacgcctac cggtacagac acaagcctca gcaagaagcc
     1261 ggcagacctc ctacagtgcc cgtggtgtat atcagacccc accagaagtg cggccccaag
     1321 gacctgttca agaagatcac cgagtacctg aagtaccgcg tgaccaaggg caccgtgtcc
     1381 gacttcagag acagaaccat cgaggtgctg aaaggctgcg gcgtggaaat gctgatcatc
     1441 gacgaggccg accggctgaa gcctgagaca tttgctgacg tgcgggatat cgccgaggac
     1501 ctgggaattg ctgtggtgct cgtgggcacc gatagactgg atgccgtgat caagcgggac
     1561 gaacaggtgc tggaacggtt cagagcccac ctgagattcg gcaagctgag cggcgaggac
     1621 ttcaagaaca cagtggaaat gtgggagcag atggtgctga agctgcccgt gtccagcaac
     1681 ctgaagtcca aagagatgct gcggatcctg accagcgcca ccgagggata tatcggcaga
     1741 ctggacgaga tcctgagaga ggccgccatc agaagcctgt ccagaggcct gaagaaaatc
     1801 gacaaggccg tgctgcaaga ggtggccaaa gagtacaagt aagcggccgc actcgaggcc
     1861 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1921 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatccc
     1981 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     2041 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     2101 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
     2161 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2221 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2581 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     2641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2881 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     2941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3541 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     4201 catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg
     4261 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     4321 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4381 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4441 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     4501 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     4561 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     4621 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
     4681 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     4741 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     4801 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     4861 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     4921 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     4981 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     5041 cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat
     5101 gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt
     5161 ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc
     5221 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     5281 cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc
     5341 catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt
     5401 aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc
     5461 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     5521 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
     5581 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     5641 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     5701 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     5761 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     5821 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     5881 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5941 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6001 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     6061 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     6121 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     6181 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     6241 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     6301 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     6361 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     6421 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     6481 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     6541 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     6601 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     6661 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     6721 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     6781 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     6841 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     6901 cgttttcgca g
//
