LOCUS       Exported                5343 bp ds-DNA     circular SYN 30-OCT-2019
DEFINITION  Junction of sequence may be not correct. Needs to be sequenced.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Mamiko Yajima
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 1, 2019 from SnapGene Viewer 5.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    7..579
                     /label=Sp SM50 promoter
     misc_feature    590..1329
                     /label=GFP
     polyA_signal    1712..1846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1971..2426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             2714..3574
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    complement(3676..3709)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      3787..4375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        4835..5138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5139..5342
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ctgcaggccg tggaaaataa ctaggcatat cttgacacgc ataaggaacg ttactttgga
       61 ggccccttcg ctcccccaac accgtcccgc gcacacgatc ttgcatgtac tccctataca
      121 cacgttcacc cacatttagc ccacgcacac actcattccc aaccaggaag tcacgctcat
      181 tatgggtaat tatgcgctca tcccaaagcc cctgactcaa gtttcccttt cggaaccccg
      241 gctaggtctg gatgtgcgtc aatcctacca tctcctctga ttggtccaaa agttacgccc
      301 cgttttcggc ttctgcgcac accccacgcg catggggcgt gcacggagat gcgttggtca
      361 cgcccccatc agagtcttgc actcggccca gggttacgcc tgttgcgcga gggttttgag
      421 gttcggctaa ggctttcatt cgagtttggt ggtagtcgtg aatgcatcga tctcatttct
      481 tctggagttc gaaaaattaa gaaagaaaaa agtctagtga gatcgcaaca catttgagaa
      541 gcagccatga agggagtcga tcgacgagct gaactagtgg cggccgccgc caccatgagc
      601 aagggcgagg aactgttcac tggcgtggtc ccaattctcg tggaactgga tggcgatgtg
      661 aatgggcaca aattttctgt cagcggagag ggtgaaggtg atgccacata cggaaagctc
      721 accctgaaat tcatctgcac cactggaaag ctccctgtgc catggccaac actggtcact
      781 accttcacct atggcgtgca gtgcttttcc agatacccag accatatgaa gcagcatgac
      841 tttttcaaga gcgccatgcc cgagggctat gtgcaggaga gaaccatctt tttcaaagat
      901 gacgggaact acaagacccg cgctgaagtc aagttcgaag gtgacaccct ggtgaataga
      961 atcgagttga agggcattga ctttaaggaa gatggaaaca ttctcggcca caagctggaa
     1021 tacaactata actcccacaa tgtgtacatc atggccgaca agcaaaagaa tggcatcaag
     1081 gtcaacttca agatcagaca caacattgag gatggatccg tgcagctggc cgaccattat
     1141 caacagaaca ctccaatcgg cgacggccct gtgctcctcc cagacaacca ttacctgtcc
     1201 acccagtctg ccctgtctaa agatcccaac gaaaagagag accacatggt cctgctggag
     1261 tttgtgaccg ctgctgggat cacacatggc atggacgagc tgtacaagtg agcgtctaga
     1321 ctcgagaagc ttgcttggga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     1381 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     1441 atgtgttaaa ctagctgcat atgcttgctg cttgagagtt ttgcttactg agtatgattt
     1501 atgaaaatat tatacacagg agctagtgat tctaattgtt tgtgtatttt agattcacag
     1561 tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca taatcagcca
     1621 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     1681 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     1741 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgatc ctgcattaat
     1861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctggcgtaat agcgaagagg
     1921 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     1981 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2041 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2101 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2161 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     2221 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2281 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     2341 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     2401 taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac
     2461 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatgc caggtcttgg
     2521 actggtgaga acggcttgct cggcagcttc gatgtgtgct ggagggagaa taaaggtcta
     2581 agatgtgcga tagagggaag tcgcattgaa ttatgtgctg tgtagggatc gctggtatca
     2641 aatatgtgtg cccacccctg gcatgagaca ataaccctga taaatgcttc aataatattg
     2701 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     2761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     2821 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     2881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     2941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3421 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3541 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3601 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3661 tgataatctc atgccataac ttcgtataat gtatgctata cgaagttatg gcatgaccaa
     3721 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3781 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3841 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3901 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3961 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4021 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4081 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4141 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     4201 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4261 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4321 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4381 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4441 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4501 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4561 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gagcttgcaa
     4621 ttcgcgcgtt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4801 tatcacgagg ccctttcact cattagatgc atgtcgttac ataacttacg gtaaatggcc
     4861 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     4921 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     4981 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     5041 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5101 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5161 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5221 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     5281 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5341 ctc
//
