LOCUS       Exported                4073 bp ds-DNA     linear   UNA 13-OCT-2017
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 1, 2019 from SnapGene Viewer 5.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..760
                     /label=Upstream region
     primer_bind     724..748
                     /label=IVT-SP6.f1
     primer_bind     730..758
                     /label=IVT-SP6.f2
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    829..832
                     /label=Sequencing insertion
     misc_feature    838..3033
                     /label=VASA ORF
     misc_feature    838..1260
                     /label=1F
     misc_feature    1086
                     /label=S83K
     misc_feature    1258..1773
                     /label=Zn finger domain
     misc_feature    1261..1446
                     /label=2F
     misc_feature    1447..1866
                     /label=4F
     misc_feature    1831..1833
                     /label=K332N
     misc_feature    1867..2496
                     /label=5F
     misc_feature    1867..2304
                     /label=DEAD box helicase domain
     primer_bind     2052..2076
                     /label=JKP-017
     misc_feature    2158..2160
                     /label=E441Q
     primer_bind     complement(2340..2351)
                     /label=JKP-065
     misc_feature    2494..2733
                     /label=Helicase conserved C-terminal domain
     misc_feature    2497..2727
                     /label=6F
     primer_bind     2508..2531
                     /label=JKP-064
     misc_feature    2644..3033
                     /label=Vasa-DEAD region
     misc_feature    2728..3033
                     /label=7F
     misc_feature    2743
                     /label=S636K
     misc_feature    2823
                     /label=P662K
     primer_bind     complement(3003..3027)
                     /label=JKP-065
     CDS             3040..3758
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVS?EGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3509..3532)
                     /label=JKP-052
     primer_bind     complement(3856..3881)
                     /label=JKP-024
     primer_bind     complement(3856..3881)
                     /label=Vasa reverse sequencing primer 1
     primer_bind     complement(3978..4001)
                     /label=B-globinR4-2
     primer_bind     complement(3994..4017)
                     /label=B-globinR4
     primer_bind     complement(4012..4035)
                     /label=M13R
     primer_bind     complement(4015..4031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4028..4051)
                     /label=M13R0
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctgatca tgccatggtt cgggcccatg
      841 tcagaagact gggggacctc ttcggtgtcg ggtggatcca gctttggaaa gagtagtttt
      901 ggtggatcaa gtggtggggt caaagacgat ggatttggcg ttggagtcgg tcgaggtcga
      961 ggaaggggtc gcgggtttag cccctttgct tcaaacggtg gtgtcggtgg tatgaccaat
     1021 gggactacca atggaggagg agagagtagc ggtgggtggg atgagagccc atctaagagt
     1081 ggctcaagta gcagtccatg gggcggtgat ggtgataatg ggttcggcaa gagtagcttt
     1141 ggtagcaaat cgttcggtgg aggaggagga ggtggtggtg gtgggggccg aggtgctgaa
     1201 tcaggaggag gacgaggagg aggaggattc ggcggcagac gaggaggacg tggaggtgga
     1261 gatagatcct gttataactg cggtgaaaca ggtcacatgt caagagaatg tcccacaaag
     1321 gactcttcag gaggaggacg tggtggtggt ggtggcgata gaagttgcta caattgcggt
     1381 gagaccggac acatgtcacg agaatgtccc acaaaagact cttcgggtgg aggaggtgga
     1441 ggtggtggaa agtgctttag atgtcaagaa gaaggccact ttgccaagga atgccccaat
     1501 ccagatacaa gaaatgaagg cgaaggtggt gaagccaagc cgccagcatc cacctacgtt
     1561 cctcccgacc catccgagga agaatcacag atttacataa gcactgaaca aggaatcaac
     1621 ttcaacaagt atgatgatat acccgtagaa gtcagcggtc gagacaggcc aaaaaatata
     1681 cgagcttttg aggaggccga gctagatgag acggtattgt ccaatgtgcg caaggctcac
     1741 tacgctaagc caacacctgt gcagaagtat ggtatgccca tcatttcttg tggacgtgat
     1801 ctgatggcct gtgcccaaac aggatcagga aagacggcgg cattcctcct acccatcatc
     1861 accaacatga tcacccacgg aggatgcatc agcaccttca acgtgatcca ggaaccactg
     1921 gccctcatcg tctcgccaac ccgcgagctg gccatccaga tctacaacga ggcacgcaag
     1981 ttctgccgcg gcaccaccat acgacctgtg gtggtatacg gaggcaccag cgtcagtcat
     2041 cagatgagag agtgtcagaa gggatgctcc atcctggtag ccaccccagg acgtatgcat
     2101 gacttcgtaa ccagaggata tatcggcctg ggcaagctga agttcctgat ccttgacgaa
     2161 gccgaccgta tgttggacat gggtttcggt cccgacatcc agagactgat cgagcaccct
     2221 cacatgcctg ccaagggtga tcgtcaaacc ctgatgttca gtgcgacctt ccccaatgag
     2281 gtccaggaga aggcagggga gtacctcaac gactacctct tcctgacggt agggagggta
     2341 ggaggagctg cctcagacat cgagcagagg gtgttctcgg tcggtcaatt cgacaagcgg
     2401 gataagctga tggagatcct gggggatcag aaagatgatg accgtactct ggtgtttgtt
     2461 tcgaccaaga ggaatgctga tttcttggcc tctcttctca gtcaatctga attccccacc
     2521 accagcatcc atggtgaccg tcagcagcag gagcgtgagg aagccctgcg tgacttcaag
     2581 accggcaagg caccaatcct tgttgccacg tccgtcgccg ccagaggtct ggacatccca
     2641 ggggtcaagc acgtggtcaa ctacgacctc ccaagcgaca tcgatgagta cgtgcatcgc
     2701 atcgggcgta ccggacgtgt cggaaacctt ggacgatcaa ctagcttcta tgatgcagaa
     2761 aaggatgcta acattgcgag agctctcatt aagatcttgg ctgatgcgca gcaggaagta
     2821 ccggagttct tagaggaggc tgctgattca gccattggaa cctaccatgg caacgctgga
     2881 gggagctttg gaggcaggga caccaggaag ttcggtggtc gaggaggagg aaggggagga
     2941 ggaagtggcg gcggtggtgg agacgagtgg ggatctggag gaggaggcgg cggcggtggc
     3001 ggaggaggag ctgctgatga tgaatcatgg gatactagta tggtgagcaa gggcgaggag
     3061 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3121 ttcagcgtgt ccgncgaggg cgagggcgat gccacntacg gcaagctgac cctgaagttc
     3181 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacntac
     3241 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3301 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3361 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3421 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3481 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3541 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3601 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3661 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3721 gccgggatca ctctcggcat ggacgagctg tacaagtaac tagtgactga ctaggatctg
     3781 gttaccacta aaccagcctc aagaacaccc gaatggagtc tctaagctac ataataccaa
     3841 cttacacttt acaaaatgtt gtcccccaaa atgtagccat tcgtatctgc tcctaataaa
     3901 aagaaagttt cttcacattc taaaaaaaaa aaaaaaaaaa aaaccccccc cccccccccc
     3961 ccccctgcag gtcgactcta gaggatcccc gggcgagctc gaattcgtaa tcatgtcata
     4021 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gag
//
