LOCUS       Exported                2549 bp ds-DNA     linear   UNA 23-JAN-2020
DEFINITION  membrane domain was removed  by APA1CAAX was insertedt at C-term by 
            Afl2 and Spe1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 23, 2020 from SnapGene 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..760
                     /label=Upstream region seqed by Kn555 & Kn554
                     
     primer_bind     724..748
                     /label=IVT-SP6.f1
     primer_bind     730..758
                     /label=IVT-SP6.f2
     promoter        736..754
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           766..808
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(804..833)
                     /label=MutagenesisR
     primer_bind     804..833
     misc_feature    823
                     /label=Change to C to remove another ORF
     CDS             831..1547
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             831..1547
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1316..1338
                     /label=eGFP-Cf1
     primer_bind     1355..1373
                     /label=eGFP-Cf2
     primer_bind     1565..1606
                     /label=InFus-ePDZf
     misc_feature    1587..2168
                     /label=ePDZb1
     primer_bind     1937..1957
                     /label=ePDZ-Cf1
     misc_feature    2153..2273
                     /label=CAAX-gBlock
     primer_bind     2181..2231
                     /label=CAAXf1
     misc_feature    2181..2219
                     /label=CAAX-associated sequence
     primer_bind     complement(2216..2261)
                     /label=CAAXr1
     misc_feature    2220..2231
                     /label=CAAX
     primer_bind     complement(2332..2357)
                     /label=SP64-Rseq1
     primer_bind     complement(2454..2477)
                     /label=B-globinR4-2
     primer_bind     complement(2470..2493)
                     /label=B-globinR4
     primer_bind     complement(2488..2511)
                     /label=M13R
     primer_bind     complement(2491..2507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2504..2527)
                     /label=M13R0
ORIGIN
        1 attaacctat aaaaaatagg cgtatcacga ggcccttttc gtctcgcgcg tttcggtgat
       61 gacggtgaaa acntntgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
      121 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
      181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc
      241 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
      301 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
      361 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
      421 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
      481 ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
      541 gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc caaccaaacc
      601 gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc cagatgctac
      661 acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      721 tatcatacac atacgattta ggtgacacta tagaatacaa gcttgcttgt tctttttgca
      781 gaagctcaga ataaacgctc aactttggca gatctatgcc atcggggccc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      961 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1321 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1441 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct atacaagtcc ggaggggatc
     1561 ggtggagctc caccggtggc ggccgcatgc cagaacttgg atttagcata tcaggtggtg
     1621 tcgggggtag aggaaaccca ttcagacctg atgatgatgg tatatttgta acaagggtac
     1681 aacctgaagg accagcatca aaattactgc agccaggtga taaaattatt caggctaatg
     1741 gctacagttt tataaatatt gaacatggac aagcagtgtc cttgctaaaa actttccaga
     1801 atacagttga actcatcatt gtacgagaag ttggtaacgg tgcaaaacaa gagattcgag
     1861 tgagggttga aaaagacggt ggttctggag gcgtttcttc tgttccgacc aatctggaag
     1921 ttgttgctgc gaccccgact agcctgctga tcagctggga tgcgtatagg gagcttccgg
     1981 ttagttatta ccgtatcacg tacggtgaaa ccggtggtaa ctccccggtt caggaattca
     2041 ctgtacctgg ttccaagtct actgctacca tcagcggcct gaaaccgggt gtcgactata
     2101 ccatcactgt atacgcacat tacaactacc attactactc tagcccaatc tcgattaact
     2161 accgtacctc tagactcgag aaggatggta agaaaaagaa gaagaagtca aaaaccaagt
     2221 gtgttatcat gtaaactagt gactgactag gatctggtta ccactaaacc agcctcaaga
     2281 acacccgaat ggagtctcta agctacataa taccaactta cactttacaa aatgttgtcc
     2341 cccaaaatgt agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa
     2401 aaaaaaaaaa aaaaaaaaac cccccccccc cccccccccc ctgcaggtcg actctagagg
     2461 atccccgggc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     2521 tccgctcaca attccacaca acatacgag
//
