LOCUS       EF1a-AausFP1-Z1         2853 bp ds-DNA     circular     05-SEP-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature                    complement(1643..2024)
                                     /label="Zeocin resistance"
                                     /ApEinfo_revcolor=#d6b295
                                     /ApEinfo_fwdcolor=#d6b295
     misc_feature                    1334..1350
                                     /label="MCS"
                                     /ApEinfo_revcolor=#faac61
                                     /ApEinfo_fwdcolor=#faac61
     misc_feature                    complement(2025..2090)
                                     /label="EM7 promoter"
                                     /ApEinfo_revcolor=#84b0dc
                                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature                    617..1321
                                     /label="AausFP1"
                                     /ApEinfo_revcolor=#9eafd2
                                     /ApEinfo_fwdcolor=#9eafd2
     polyA_signal                    1361..1552
                                     /label="SV40 late polyA"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     Bacterial origin of replication complement(2097..2853)
                                     /label="pMB1 ori"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature                    complement(7..18)
                                     /label="U6-gRNA-tRNA cloning site"
                                     /ApEinfo_revcolor=#d59687
                                     /ApEinfo_fwdcolor=#d59687
     Promoter                        24..567
                                     /label="hEF1-HTLV promoter"
                                     /ApEinfo_revcolor=#c6c9d1
                                     /ApEinfo_fwdcolor=#c6c9d1
     transcriptional terminator      complement(1591..1642)
                                     /label="T0 bacterial terminator"
                                     /ApEinfo_revcolor=#f58a5e
                                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature                    1352..1585
                                     /label="SV40 poly(A)"
                                     /ApEinfo_revcolor=#b1ff67
                                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature                    611..619
                                     /label="Kozak"
                                     /ApEinfo_revcolor=#b7e6d7
                                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 CTGCAGAAGC TTTACTAACT AGTGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA
       61 GCGCACATCG CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC
      121 CTAGAGAAGG TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT
      181 TCCCGAGGGT GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG
      241 CAACGGGTTT GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG
      301 CGCCCGCCGC CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC
      361 CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG
      421 ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT
      481 TTGCCTGACC CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA
      541 CAGATCCAAG CTGTGACCGG CGCCTACAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA
      601 TCCACCGGTC GCCACCATGG GTTCCTATGG TGCTCTGCTG TTTAGGGAGA AGATACCTTA
      661 CGTGGTCGAA ATGGAAGGCG ACGTCGAAGG CATGAAATTC AGCGTGCGGG GCAAGGGGCA
      721 CGGAGATGCC AATACAGGGA AGATCGAGGC TTCCTTTATA TGTACTACCG GCGAGCTGCC
      781 TGTTCCCTGG AGCTCTATAC TCACCACCGT GACCTATGGT GCCCAATGCT TCGCTAAGTA
      841 CCCGAACGAC ATAAAGGATT ATCCTAAGTC CGCAATGCCC GAGGGGTATG TCCAGGAACG
      901 TACCATAACA TTCGAGAATG ATGGAGTTTA CAAAACGCGG GCCGAGGTTA CTTATGAAAA
      961 GGGCTCCGTC TACAATAGGG TGACACTGAA TGGGTCCGGG TTCAAGAAGG GCGGAAATAT
     1021 TCTGGGAAAG AAGCTGGAGT TCAATTATAA TCCCCACTGT ATTTACGTCC TGCCCGACGT
     1081 TCAGAACAAC GGCATTAAGT GTTATATTAA TATCGTGCAC GATGTGATCG GCGGCGGTCA
     1141 GATCATCGCA GCCCATCAGC AGCTGAACAC ACCACTCGGC GGCGGTCCCG TAGATATCCC
     1201 GCATTATCAC CACATCCAGG CCCATACCAT CCTCTCAAAG GACCCGAAGG AGACCAGGGA
     1261 CCACATGAAT GTGGTCGAAG TATTTCGTGC CATTGACTGT AAGACTGCTT ACGCCTGATA
     1321 GGCGGCCGCG ACTCTAGATG TACACATATG GCTAGCTGGC CAGACATGAT AAGATACATT
     1381 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT
     1441 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC
     1501 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
     1561 TAAAACCTCT ACAAATGTGG TATGGAATTC tggactcaca aagaaaaaac gcccggtgtg
     1621 caagaccgag cgttctgaac aaTTTATCCT CAGTCCTGCT CCTCTGCCAC AAAGTGCACG
     1681 CAGTTGCCGG CCGGGTCGCG CAGGGCGAAC TCCCGCCCCC ACGGCTGCTC GCCGATCTCG
     1741 GTCATGGCCG GCCCGGAGGC GTCCCGGAAG TTCGTGGACA CGACCTCCGA CCACTCGGCG
     1801 TACAGCTCGT CCAGGCCGCG CACCCACACC CAGGCCAGGG TGTTGTCCGG CACCACCTGG
     1861 TCCTGGACCG CGCTGATGAA CAGGGTCACG TCGTCCCGGA CCACACCGGC GAAGTCGTCC
     1921 TCCACGAAGT CCCGGGAGAA CCCGAGCCGG TCGGTCCAGA ACTCGACCGC TCCGGCGACG
     1981 TCGCGCGCGG TGAGCACCGG AACGGCACTG GTCAACTTGG CCATGATGGC CCTCCTATAG
     2041 TGAGTCGTAT TATACTATGC CGATATACTA TGCCGATGAT TAATTGTCAA GGATCCTAAG
     2101 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG
     2161 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG
     2221 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG
     2281 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA
     2341 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC
     2401 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT
     2461 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT
     2521 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG
     2581 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT
     2641 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT
     2701 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT
     2761 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     2821 GGCTAGTTAA TTAACATTTA AATCAGCGGC CGC
//