LOCUS       Exported               10205 bp ds-DNA    circular SYN 25-NOV-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pQlox71R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 25 Nov 2019 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3439..4176
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          4911..7229
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          7620..8204
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    122..1921
                     /label="LtrA"
                     /note="LtrA"
                     /note="color: #ff80c0; direction: RIGHT"
     misc_feature    2330..3105
                     /label="oriT (pRK24)"
                     /note="oriT"
                     /note="color: #ff8000"
     CDS             3439..4176
                     /codon_start=1
                     /label="ermR"
                     /note="ermR"
                     /note="color: #ffff80"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTITYEQVLSIFNSYLLFNGRK"
     CDS             4911..5210
                     /codon_start=1
                     /transl_table=11
                     /product="RepD"
                     /note="RepD"
                     /note="color: #993366"
                     /protein_id="AAQ75108.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKSD
                     KYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     CDS             5253..6743
                     /codon_start=1
                     /transl_table=11
                     /product="RepE"
                     /note="RepE"
                     /note="plasmid replication; pAMbeta1 plasmid replicon"
                     /note="color: #993366"
                     /protein_id="AAQ75109.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     CDS             7093..7229
                     /codon_start=1
                     /transl_table=11
                     /product="RepG"
                     /note="RepG"
                     /note="color: #993366"
                     /protein_id="AAQ75110.1"
                     /translation="MEKYLKNPITWIGLVLVVTWFLTKSSEFLIFGVCVLLLVFASQSD
                     "
     misc_feature    7620..8204
                     /label="oriR(pUC)"
                     /note="ori pUC"
                     /note="color: #80ffff"
     misc_feature    8632..9056
                     /label="pFer"
                     /note="pFer"
                     /note="color: #800000; direction: RIGHT"
     misc_feature    9057..9413
                     /label="retargeted intron"
                     /note="retargeted intron"
                     /note="color: #80ff80; direction: RIGHT"
     misc_feature    9791..9796
                     /note="MluI"
                     /note="color: #ff0000"
     misc_feature    complement(9797..9830)
                     /label="Lox71"
                     /note="Lox71"
                     /note="color: #0080c0; direction: LEFT"
     misc_feature    9831..9836
                     /note="MluI"
                     /note="color: #ff0000"
ORIGIN
        1 tgcgttgatg caatttctat gcactcgtag tagtctgaga agggtaacgc cctttacatg
       61 gcaaaggggt acagttattg tgtactaaaa ttaaaaattg attagggagg aaaacctcaa
      121 aatgaaacca acaatggcaa ttttagaaag aatcagtaaa aattcacaag aaaatataga
      181 cgaagttttt acaagacttt atcgttatct tttacgtcca gatatttatt acgtggcgta
      241 tcaaaattta tattccaata aaggagcttc cacaaaagga atattagatg atacagcgga
      301 tggctttagt gaagaaaaaa taaaaaagat tattcaatct ttaaaagacg gaacttacta
      361 tcctcaacct gtacgaagaa tgtatattgc aaaaaagaat tctaaaaaga tgagaccttt
      421 aggaattcca actttcacag ataaattgat ccaagaagct gtgagaataa ttcttgaatc
      481 tatctatgaa ccggtattcg aagatgtgtc tcacggtttt agacctcaac gaagctgtca
      541 cacagctttg aaaacaatca aaagagagtt tggcggcgca agatggtttg tggagggaga
      601 tataaaaggc tgcttcgata atatagacca cgttacactc attggactca tcaatcttaa
      661 aatcaaagat atgaaaatga gccaattgat ttataaattt ctaaaagcag gttatctgga
      721 aaactggcag tatcacaaaa cttacagcgg aacacctcaa ggtggaattc tatctcctct
      781 tttggccaac atctatcttc atgaattgga taagtttgtt ttacaactca aaatgaagtt
      841 tgaccgagaa agtccagaaa gaataacacc tgaatatcgg gagctccaca atgagataaa
      901 aagaatttct caccgtctca agaagttgga gggtgaagaa aaagctaaag ttcttttaga
      961 atatcaagaa aaacgtaaaa gattacccac actcccctgt acctcacaga caaataaagt
     1021 attgaaatac gtccggtatg cggacgactt cattatctct gttaaaggaa gcaaagagga
     1081 ctgtcaatgg ataaaagaac aattaaaact ttttattcat aacaagctaa aaatggaatt
     1141 gagtgaagaa aaaacactca tcacacatag cagtcaaccc gctcgttttc tgggatatga
     1201 tatacgagta aggagatctg gaacgataaa acgatctggt aaagtcaaaa agagaacact
     1261 caatgggagt gtagaactcc ttattcctct tcaagacaaa attcgtcaat ttatttttga
     1321 caagaaaata gctatccaaa agaaagatag ctcatggttt ccagttcaca ggaaatatct
     1381 tattcgttca acagacttag aaatcatcac aatttataat tctgaactcc gcgggatttg
     1441 taattactac ggtctagcaa gtaattttaa ccagctcaat tattttgctt atcttatgga
     1501 atacagctgt ctaaaaacga tagcctccaa acataaggga acactttcaa aaaccatttc
     1561 catgtttaaa gatggaagtg gttcgtgggg gatcccgtat gagataaagc aaggtaagca
     1621 gcgccgttat tttgcaaatt ttagtgaatg taaatcccct tatcaattta cggatgagat
     1681 aagtcaagct cctgtattgt atggctatgc ccggaatact cttgaaaaca ggttaaaagc
     1741 taaatgttgt gaattatgtg ggacgtctga tgaaaatact tcctatgaaa ttcaccatgt
     1801 caataaggtc aaaaatctta aaggcaaaga aaaatgggaa atggcaatga tagcgaaaca
     1861 acgtaaaact cttgttgtat gctttcattg tcatcgtcac gtgattcata aacacaagtg
     1921 agatgggtac cctcgaggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     1981 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2041 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2101 cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2161 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2221 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2281 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccggat cggtcttgcc
     2341 ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat
     2401 ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg
     2461 gctctgccct cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt
     2521 cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt
     2581 cggtgagcca gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca
     2641 gctcgcggac gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca
     2701 gcaggtaggc cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt
     2761 cgtctggaag gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat
     2821 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     2881 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     2941 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     3001 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     3061 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatccgt cgaccttaga
     3121 agcaaactta agagtgtgtt gatagtgcag tatcttaaaa ttttgtgtat aataggaatt
     3181 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
     3241 tccaaatgcg taatgtagat aaaacatcta ctgttttgaa acagactaaa aacagtgatt
     3301 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
     3361 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta cagatgtaat
     3421 cacttcagga gtaattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
     3481 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
     3541 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
     3601 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
     3661 attcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
     3721 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
     3781 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
     3841 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
     3901 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
     3961 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
     4021 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
     4081 atgaaacacg ccaaagtaaa caatttaagt accattactt atgagcaagt attgtctatt
     4141 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttttaaatt
     4201 tggaaagtta cacgttacta aagggaatgg agataaatta ttagatatac tactgacagc
     4261 ttccaagaag ctaaagaggt ccctagcgcc tacggggaat ttgtaaatca aaagaaaaat
     4321 tctcccaaga attagaaatg agtagatcaa attattcacg aatagaatca ggaaaatcag
     4381 atccaaccat aaaaacacta gaacaaattg caaagttaac taactcaacg ctagtagtgg
     4441 atttaatccc aaatgagcca acagaaccag aaccagaaac agaatcagaa caagtaacat
     4501 tggatttaga aatggaagaa gaaaaaagca atgacttcgt gtgaataatg cacgaaatcg
     4561 ttgcttattt ttttttaaaa gcggtatact agatataacg aaacaacgaa ctgaatagaa
     4621 acgaaaaaag agccatgaca catttataaa atgtttgacg acattttata aatgcatagc
     4681 ccgataagat tgccaaacca acgcttatca gttagtcaga tgaactcttc cctcgtaaga
     4741 agttatttaa ttaactttgt ttgaagacgg tatataaccg tactatcatt atatagggaa
     4801 atcagagagt tttcaagtat ctaagctact gaatttaaga attgttaagc aatcaatcgg
     4861 aaatcgtttg attgcttttt ttgtattcat ttatagaagg tggagtttgt atgaatcatg
     4921 atgaatgtaa aacttatata aaaaatagtt tattggagat aagaaaatta gcaaatatct
     4981 atacactaga aacgtttaag aaagagttag aaaagagaaa tatctactta gaaacaaaat
     5041 cagataagta tttttcttcg gagggggaag attatatata taagttaata gaaaataaca
     5101 aaataattta ttcgattagt ggaaaaaaat tgacttataa aggaaaaaaa tctttttcaa
     5161 aacatgcaat attgaaacag ttgaatgaaa aagcaaacca agttaattaa acaacctatt
     5221 ttataggatt tataggaaag gagaacagct gaatgaatat cccttttgtt gtagaaactg
     5281 tgcttcatga cggcttgtta aagtacaaat ttaaaaatag taaaattcgc tcaatcacta
     5341 ccaagccagg taaaagcaaa ggggctattt ttgcgtatcg ctcaaaatca agcatgattg
     5401 gcggtcgtgg tgttgttctg acttccgagg aagcgattca agaaaatcaa gatacattta
     5461 cacattggac acccaacgtt tatcgttatg gaacgtatgc agacgaaaac cgttcataca
     5521 cgaaaggaca ttctgaaaac aatttaagac aaatcaatac cttctttatt gattttgata
     5581 ttcacacggc aaaagaaact atttcagcaa gcgatatttt aacaaccgct attgatttag
     5641 gttttatgcc tactatgatt atcaaatctg ataaaggtta tcaagcatat tttgttttag
     5701 aaacgccagt ctatgtgact tcaaaatcag aatttaaatc tgtcaaagca gccaaaataa
     5761 tttcgcaaaa tatccgagaa tattttggaa agtctttgcc agttgatcta acgtgtaatc
     5821 attttggtat tgctcgcata ccaagaacgg acaatgtaga attttttgat cctaattacc
     5881 gttattcttt caaagaatgg caagattggt ctttcaaaca aacagataat aagggcttta
     5941 ctcgttcaag tctaacggtt ttaagcggta cagaaggcaa aaaacaagta gatgaaccct
     6001 ggtttaatct cttattgcac gaaacgaaat tttcaggaga aaagggttta atagggcgta
     6061 ataacgtcat gtttaccctc tctttagcct actttagttc aggctattca atcgaaacgt
     6121 gcgaatataa tatgtttgag tttaataatc gattagatca acccttagaa gaaaaagaag
     6181 taatcaaaat tgttagaagt gcctattcag aaaactatca aggggctaat agggaataca
     6241 ttaccattct ttgcaaagct tgggtatcaa gtgatttaac cagtaaagat ttatttgtcc
     6301 gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg tcaacgtgtt catttgtcag
     6361 aatggaaaga agatttaatg gcttatatta gcgaaaaaag cgatgtatac aagccttatt
     6421 tagtgacgac caaaaaagag attagagaag tgctaggcat tcctgaacgg acattagata
     6481 aattgctgaa ggtactgaag gcgaatcagg aaattttctt taagattaaa ccaggaagaa
     6541 atggtggcat tcaacttgct agtgttaaat cattgttgct atcgatcatt aaagtaaaaa
     6601 aagaagaaaa agaaagctat ataaaggcgc tgacaaattc ttttgactta gagcatacat
     6661 tcattcaaga gactttaaac aagctagcag aacgccctaa aacggacaca caactcgatt
     6721 tgtttagcta tgatacaggc tgaaaataaa acccgcacta tgccattaca tttatatcta
     6781 tgatacgtgt ttgttttttc tttgctgttt agcgaatgat tagcagaaat atacagagta
     6841 agattttaat taattattag ggggagaagg agagagtagc ccgaaaactt ttagttggct
     6901 tggactgaac gaagtgaggg aaaggctact aaaacgtcga ggggcagtga gagcgaagcg
     6961 aacacttgat tttttaattt tctatctttt ataggtcatt agagtatact tatttgtcct
     7021 ataaactatt tagcagcata atagatttat tgaataggtc atttaagttg agcatattag
     7081 aggaggaaaa tcttggagaa atatttgaag aacccgatta catggattgg attagttctt
     7141 gtggttacgt ggtttttaac taaaagtagt gaatttttga tttttggtgt gtgtgtcttg
     7201 ttgttagtat ttgctagtca aagtgattaa ataggaccac ttctgcgctc ggcccttccg
     7261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7981 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8401 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8461 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8521 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8581 taacaatttc acacaggaaa cagctatgac catgattaca agcttgcatg cctgcagttt
     8641 ggtcaacatt taacctctaa tcttctagtt catttatatg tccatgaatt gaatttcata
     8701 catagttttc aattgtatgg atgtagtatg acagaaaatc aaaaaatcat acttattata
     8761 tttaggtagt tactgttaat ttttagtatg ttaatttcta atctgagttc tttccgtatt
     8821 ataataagtg taaagatcac accatatttt tacagttttt acacgtttca ttaataattt
     8881 cttatatttc tttatttgtt tgtaaaattt acttaaattt cgccagaaaa caaaagaaag
     8941 cctttactaa ttaatagttt agtgatactc ttttatgtag gtatttttta aaatacatta
     9001 aacctaggta attgaggaaa gttacaatta ccattatata aggaggatat tcaaaccata
     9061 tgggatcccc agcttcgtcg atcgtgaaga tttaatcaat gtgcgcccag atagggtgtt
     9121 aagtcaagta gtttaaggta ctactctgta agataacaca gaaaacagcc aacctaaccg
     9181 aaaagcgaaa gctgatacgg gaacagagca cggttggaaa gcgatgagtt acctaaagac
     9241 aatcgggtac gactgagtcg caatgttaat cagatataag gtataagttg tgtttactga
     9301 acgcaagttt ctaatttcgg ttaaatctcg atagaggaaa gtgtctgaaa cctctagtac
     9361 aaagaaaggt aagttagcat tgatgactta tctgttatca ccacatttgt acaatctgta
     9421 ggagaaccta tgggaacgaa acgaaagcga tgccgagaat ctgaatttac caagacttaa
     9481 cactaactgg ggatacccta aacaagaatg cctaatagaa aggaggaaaa aggctatagc
     9541 actagagctt gaaaatcttg caagggtacg gagtactcgt agtagtctga gaagggtaac
     9601 gccctttaca tggcaaaggg gtacagttat tgtgtactaa aattaaaaat tgattaggga
     9661 ggaaaacctc aaaatgaaac caacaatggc aattttagaa agaatcagta aaaattcaca
     9721 agaaaatata gacgaagttt ttacaagact ttatcgttat cttttacgtc cagatattta
     9781 ttacgtggcg acgcgtataa cttcgtataa tgtatgctat acgaacggta acgcgttggg
     9841 aaatggcaat gatagcgaaa caacgtaaaa ctcttgttgt atgctttcat tgtcatcgtc
     9901 acgtgattca taaacacaag tgaatgtcga ctaatacgac tcactatagg gtcgacagtg
     9961 aatttttacg aacgaacaat aacagagccg tatactccga gaggggtacg tacggttccc
    10021 gaagagggtg gtgcaaacca gtcacagtaa tgtgaacaag gcggtacctc cctacttcac
    10081 catatcattt tctgcagccc cctagaaata attttgttta actttaagaa ggagatatac
    10141 atatatggct agatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
    10201 ctata
//
