LOCUS Exported 9249 bp ds-DNA circular SYN 17-FEB-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9249) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Feb 17, 2020 from SnapGene Viewer 5.0.4 https://www.snapgene.com COMMENT > Addgene NGS Result FEATURES Location/Qualifiers source 1..9249 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 139..336 /locus_tag="f1\(+)\ori\frag" /label=f1\(+)\ori\frag misc_feature 807..1153 /locus_tag="Tol2(1)" /label=Tol2 /label=Tol2(1) misc_feature 1328..1497 /locus_tag="SV40 polyA" /label=SV40 polyA misc_feature 1530..2102 /locus_tag="bGlobin intron" /label=bGlobin intron misc_feature complement(2157..2891) /locus_tag="TagRFP-T cds" /label=TagRFP-T cds misc_feature 2157..2159 /locus_tag="STOP(1)" /label=STOP /label=STOP(1) misc_feature 2889..2891 /locus_tag="ATG(1)" /label=ATG /label=ATG(1) misc_feature complement(2892..2942) /locus_tag="Fyn Tyrosinase Tag" /label=Fyn Tyrosinase Tag misc_feature complement(2940..2942) /locus_tag="ATG(3)" /label=ATG /label=ATG(3) misc_feature 2940..2942 /locus_tag="ATG(4)" /label=ATG /label=ATG(4) misc_feature 2943..2947 /locus_tag="kozak" /label=kozak misc_feature 2974..3208 /locus_tag="E1b-5xUAS-E1b" /label=E1b-5xUAS-E1b misc_feature complement(3217..3223) /locus_tag="kozak(1)" /label=kozak /label=kozak(1) CDS 3224..3943 /locus_tag="eGFP" /label=eGFP misc_feature complement(3224..3226) /locus_tag="ATG(2)" /label=ATG /label=ATG(2) misc_feature complement(3941..3943) /locus_tag="STOP(2)" /label=STOP /label=STOP(2) misc_feature 4006..4578 /locus_tag="bGlobin intron(1)" /label=bGlobin intron /label=bGlobin intron(1) misc_feature 4611..4780 /locus_tag="SV40polyA" /label=SV40polyA terminator 4929..5048 /locus_tag="SV40 PA terminator" /label=SV40 PA terminator polyA_signal 5159..5291 /locus_tag="SV40\polyA" /label=SV40\polyA /note="/vntifkey=25" misc_feature complement(5429..5431) /locus_tag="STOP" /label=STOP CDS complement(5432..6148) /locus_tag="Cerulean" /label=Cerulean /note="/vntifkey=4" misc_feature complement(6146..6148) /locus_tag="ATG" /label=ATG promoter complement(6149..6455) /locus_tag="alpha-crystallin\promoter" /label=alpha-crystallin\promoter /note="/vntifkey=30" misc_feature 6462..6796 /locus_tag="Tol2" /label=Tol2 /note="/vntifkey=21" misc_feature 7450..8117 /locus_tag="pUC\ori" /label=pUC\ori CDS complement(8268..9125) /locus_tag="AmpR" /label=AmpR /note="/vntifkey=4" ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg 361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc 421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga 481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca 541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt 601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt 661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac 721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag 781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa 841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta 901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac 961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa 1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta 1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa 1141 acacaggcca gatgggccct cgagcccgac gtcgcatgct cccggccgcc atggcggccg 1201 cgggaattcg attcacacac gccgtaccgg gcccaatgca ttggcgccgc ggccgcgaat 1261 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 1321 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 1381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 1441 cttatcatgt ctggatctac gatccgtcga ggaattaatt ctttgccaaa atgatgagac 1501 agcacaacaa ccagcacgtt gcccaggagc tgtaggaaag agaagaaggc atgaacatgg 1561 ttagcagagg ggcccggttt ggactcagag tattttatcc tcatctcaaa cagtgtatat 1621 cattgtaacc ataaagagaa aggcaggatg atgaccaggg tgtagttgtt tctaccaata 1681 agaatatttc cacgccagcc agaatttata tgcagaaata ttctacctta tcatttaatt 1741 ataacaattg ttctctaaaa ctgtgctgaa gtacaatata atataccctg attgccttga 1801 aaaaaaagtg attagagaaa gtacttacaa tctgacaaat aaacaaaagt gaatttaaaa 1861 attcgttaca aatgcaagct aaagtttaac gaaaaagtta cagaaaatga aaagaaaata 1921 agaggagaca atggttgtca acagagtaga aagtgaaaga aacaaaatta tcatgagggt 1981 ccatggtgat acaagggaca tcttcccatt ctaaacaaca ccctgaaaac tttgccccct 2041 ccatataaca tgaattttac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc 2101 accctgaagt tctcaggatc ggtcgagtaa tacgactcac tatagttcta gctagattac 2161 ttgtacagct cgtccatgcc attaagtttg tgccccagtt tgctagggag gtcgcagtat 2221 ctggccacag ccacctcgtg ctgctcgacg taggtctctt tgtcggcctc cttgattctt 2281 tccagtctgt ggtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg 2341 gatctgtatg tggtcttgaa gttgcagatc aggtggcccc cgcccacgag cttcagggcc 2401 atgtcggttc tgccttccag gccgccgtca gcggggtaca gcatctcggt gttggcctcc 2461 cagccgagtg ttttcttctg catcacaggg ccgttggatg ggaagttcac ccctctgatc 2521 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg 2581 cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgctta 2641 aagaagtcgg ggatgccctg ggtgtggttg atgaaggttc tgctgccgta catgaagctg 2701 gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg 2761 gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtggtggttg 2821 ttcacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc 2881 ttagacacca tcctcgacgt cagttttgtt gcttctttat ccttacattg cacacagccc 2941 atggtggaat tgttaattaa tttaaatcaa ttcgtgtgga ggagctcaaa gtgaggctga 3001 gacgcgatgg gatccattat ataccctcta gatccatgcc tgcaggtcgg agtactgtcc 3061 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc 3121 gagcggagta ctgtcctccg agcggagact ctagagggta tataatggat cccatcgcgt 3181 ctcagcctca ctttgagctc ctccacacga attcgccgcc accatggtga gcaagggcga 3241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 3301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 3361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 3421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 3481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 3541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 3601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 3661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 3721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 3781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 3841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 3901 cgccgccggg atcactctcg gcatggacga gctgtacaag taactcgagc ctctagctag 3961 aactatagtg agtcgtatta ctcgaccgat cctgagaact tcagggtgag tttggggacc 4021 cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt 4081 ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa 4141 ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca 4201 ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact 4261 tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag 4321 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa 4381 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac 4441 aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt 4501 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc 4561 ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt 4621 ggcaaagaat taattcctcg acggatcgta gatccagaca tgataagata cattgatgag 4681 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 4741 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 4801 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccggc 4861 cgcggcgcca atgcattggg cccggtacct ctagaactat agtgagtcgt attacgtaga 4921 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 4981 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 5041 caataaacaa gttaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 5101 ggaggttttt taattcgcgg ccgcggcgac ctgcagcctg ttgacaattt tcgatgatga 5161 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 5221 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 5281 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 5341 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc 5401 tctagatcag atccagagtc gcggccgctt acttgtacag ctcgtccatg ccgagagtga 5461 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt 5521 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc 5581 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt 5641 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt 5701 tgtcgctgat ggcgttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt 5761 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc 5821 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg 5881 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact 5941 gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg 6001 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca 6061 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg 6121 tgaacagctc ctcgcccttg ctcaccatgg tacccccgca agtctgactg agtggaagtt 6181 tcagttcaat ggaagggaga aaagagccag cctttatata tgtcatgtat tgctgctgtc 6241 tgcatgcgat gaacctagag cttacttttt ctgtcaagta aggcaaaagc agagaacctt 6301 tgtcttgcat tgtgtttgat gttgtgctgt gacagtcatt tcttagttca tgctggtact 6361 tttgttccat agctttaccc aaagagttat ccagcattcc cagttattcc aaaaactcaa 6421 tgtcacaaat tttccagtcc attgcttagc agccgagatc tgcgaagata cggccacggg 6481 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat 6541 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg 6601 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg 6661 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa 6721 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa 6781 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta 6841 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact 6901 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc 6961 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta 7021 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta 7081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt 8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9241 cgaaaagtg //