LOCUS       Exported                9249 bp ds-DNA     circular SYN 17-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Feb 17, 2020 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
COMMENT     > Addgene NGS Result
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    139..336
                     /locus_tag="f1\(+)\ori\frag"
                     /label=f1\(+)\ori\frag
     misc_feature    807..1153
                     /locus_tag="Tol2(1)"
                     /label=Tol2
                     /label=Tol2(1)
     misc_feature    1328..1497
                     /locus_tag="SV40 polyA"
                     /label=SV40 polyA
     misc_feature    1530..2102
                     /locus_tag="bGlobin intron"
                     /label=bGlobin intron
     misc_feature    complement(2157..2891)
                     /locus_tag="TagRFP-T cds"
                     /label=TagRFP-T cds
     misc_feature    2157..2159
                     /locus_tag="STOP(1)"
                     /label=STOP
                     /label=STOP(1)
     misc_feature    2889..2891
                     /locus_tag="ATG(1)"
                     /label=ATG
                     /label=ATG(1)
     misc_feature    complement(2892..2942)
                     /locus_tag="Fyn Tyrosinase Tag"
                     /label=Fyn Tyrosinase Tag
     misc_feature    complement(2940..2942)
                     /locus_tag="ATG(3)"
                     /label=ATG
                     /label=ATG(3)
     misc_feature    2940..2942
                     /locus_tag="ATG(4)"
                     /label=ATG
                     /label=ATG(4)
     misc_feature    2943..2947
                     /locus_tag="kozak"
                     /label=kozak
     misc_feature    2974..3208
                     /locus_tag="E1b-5xUAS-E1b"
                     /label=E1b-5xUAS-E1b
     misc_feature    complement(3217..3223)
                     /locus_tag="kozak(1)"
                     /label=kozak
                     /label=kozak(1)
     CDS             3224..3943
                     /locus_tag="eGFP"
                     /label=eGFP
     misc_feature    complement(3224..3226)
                     /locus_tag="ATG(2)"
                     /label=ATG
                     /label=ATG(2)
     misc_feature    complement(3941..3943)
                     /locus_tag="STOP(2)"
                     /label=STOP
                     /label=STOP(2)
     misc_feature    4006..4578
                     /locus_tag="bGlobin intron(1)"
                     /label=bGlobin intron
                     /label=bGlobin intron(1)
     misc_feature    4611..4780
                     /locus_tag="SV40polyA"
                     /label=SV40polyA
     terminator      4929..5048
                     /locus_tag="SV40 PA terminator"
                     /label=SV40 PA terminator
     polyA_signal    5159..5291
                     /locus_tag="SV40\polyA"
                     /label=SV40\polyA
                     /note="/vntifkey=25"
     misc_feature    complement(5429..5431)
                     /locus_tag="STOP"
                     /label=STOP
     CDS             complement(5432..6148)
                     /locus_tag="Cerulean"
                     /label=Cerulean
                     /note="/vntifkey=4"
     misc_feature    complement(6146..6148)
                     /locus_tag="ATG"
                     /label=ATG
     promoter        complement(6149..6455)
                     /locus_tag="alpha-crystallin\promoter"
                     /label=alpha-crystallin\promoter
                     /note="/vntifkey=30"
     misc_feature    6462..6796
                     /locus_tag="Tol2"
                     /label=Tol2
                     /note="/vntifkey=21"
     misc_feature    7450..8117
                     /locus_tag="pUC\ori"
                     /label=pUC\ori
     CDS             complement(8268..9125)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="/vntifkey=4"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcccgac gtcgcatgct cccggccgcc atggcggccg
     1201 cgggaattcg attcacacac gccgtaccgg gcccaatgca ttggcgccgc ggccgcgaat
     1261 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1321 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1381 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1441 cttatcatgt ctggatctac gatccgtcga ggaattaatt ctttgccaaa atgatgagac
     1501 agcacaacaa ccagcacgtt gcccaggagc tgtaggaaag agaagaaggc atgaacatgg
     1561 ttagcagagg ggcccggttt ggactcagag tattttatcc tcatctcaaa cagtgtatat
     1621 cattgtaacc ataaagagaa aggcaggatg atgaccaggg tgtagttgtt tctaccaata
     1681 agaatatttc cacgccagcc agaatttata tgcagaaata ttctacctta tcatttaatt
     1741 ataacaattg ttctctaaaa ctgtgctgaa gtacaatata atataccctg attgccttga
     1801 aaaaaaagtg attagagaaa gtacttacaa tctgacaaat aaacaaaagt gaatttaaaa
     1861 attcgttaca aatgcaagct aaagtttaac gaaaaagtta cagaaaatga aaagaaaata
     1921 agaggagaca atggttgtca acagagtaga aagtgaaaga aacaaaatta tcatgagggt
     1981 ccatggtgat acaagggaca tcttcccatt ctaaacaaca ccctgaaaac tttgccccct
     2041 ccatataaca tgaattttac aatagcgaaa aagaaagaac aatcaagggt ccccaaactc
     2101 accctgaagt tctcaggatc ggtcgagtaa tacgactcac tatagttcta gctagattac
     2161 ttgtacagct cgtccatgcc attaagtttg tgccccagtt tgctagggag gtcgcagtat
     2221 ctggccacag ccacctcgtg ctgctcgacg taggtctctt tgtcggcctc cttgattctt
     2281 tccagtctgt ggtccacata gtagacgccg ggcatcttga ggttcttagc gggtttcttg
     2341 gatctgtatg tggtcttgaa gttgcagatc aggtggcccc cgcccacgag cttcagggcc
     2401 atgtcggttc tgccttccag gccgccgtca gcggggtaca gcatctcggt gttggcctcc
     2461 cagccgagtg ttttcttctg catcacaggg ccgttggatg ggaagttcac ccctctgatc
     2521 ttgacgttgt agatgaggca gccgtcctgg aggctggtgt cctgggtagc ggtcagcacg
     2581 cccccgtctt cgtatgtggt gactctctcc catgtgaagc cctcagggaa ggactgctta
     2641 aagaagtcgg ggatgccctg ggtgtggttg atgaaggttc tgctgccgta catgaagctg
     2701 gtagccagga tgtcgaaggc gaaggggaga gggccgccct cgaccacctt gattctcatg
     2761 gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg tgcacttgaa gtggtggttg
     2821 ttcacggtgc cctccatgta cagcttcatg tgcatgttct ccttaatcag ctcttcgccc
     2881 ttagacacca tcctcgacgt cagttttgtt gcttctttat ccttacattg cacacagccc
     2941 atggtggaat tgttaattaa tttaaatcaa ttcgtgtgga ggagctcaaa gtgaggctga
     3001 gacgcgatgg gatccattat ataccctcta gatccatgcc tgcaggtcgg agtactgtcc
     3061 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
     3121 gagcggagta ctgtcctccg agcggagact ctagagggta tataatggat cccatcgcgt
     3181 ctcagcctca ctttgagctc ctccacacga attcgccgcc accatggtga gcaagggcga
     3241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3901 cgccgccggg atcactctcg gcatggacga gctgtacaag taactcgagc ctctagctag
     3961 aactatagtg agtcgtatta ctcgaccgat cctgagaact tcagggtgag tttggggacc
     4021 cttgattgtt ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt
     4081 ttcagggtgt tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa
     4141 ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca
     4201 ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact
     4261 tttgtttatt tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag
     4321 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
     4381 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
     4441 aactacaccc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
     4501 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
     4561 ttcttctctt tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt
     4621 ggcaaagaat taattcctcg acggatcgta gatccagaca tgataagata cattgatgag
     4681 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4741 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4801 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccggc
     4861 cgcggcgcca atgcattggg cccggtacct ctagaactat agtgagtcgt attacgtaga
     4921 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     4981 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5041 caataaacaa gttaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     5101 ggaggttttt taattcgcgg ccgcggcgac ctgcagcctg ttgacaattt tcgatgatga
     5161 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     5221 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5281 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     5341 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     5401 tctagatcag atccagagtc gcggccgctt acttgtacag ctcgtccatg ccgagagtga
     5461 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     5521 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     5581 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     5641 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
     5701 tgtcgctgat ggcgttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     5761 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     5821 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     5881 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
     5941 gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     6001 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     6061 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     6121 tgaacagctc ctcgcccttg ctcaccatgg tacccccgca agtctgactg agtggaagtt
     6181 tcagttcaat ggaagggaga aaagagccag cctttatata tgtcatgtat tgctgctgtc
     6241 tgcatgcgat gaacctagag cttacttttt ctgtcaagta aggcaaaagc agagaacctt
     6301 tgtcttgcat tgtgtttgat gttgtgctgt gacagtcatt tcttagttca tgctggtact
     6361 tttgttccat agctttaccc aaagagttat ccagcattcc cagttattcc aaaaactcaa
     6421 tgtcacaaat tttccagtcc attgcttagc agccgagatc tgcgaagata cggccacggg
     6481 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     6541 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     6601 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     6661 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     6721 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     6781 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     6841 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     6901 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     6961 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     7021 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     7081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt
     8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9241 cgaaaagtg
//
