LOCUS       Exported                9551 bp ds-DNA     circular SYN 19-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Andrew S. Flies
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    459..477
                     /label=ori.REV
     misc_feature    777..1001
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1002..1043
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    1054..1354
                     /label=rBG-pA
                     /note="rBG-pA;rabbit beta-globin polyadenylation 
                     signal;color: #cc0000; direction: RIGHT"
     misc_feature    1068..1092
                     /label=rBG-pA_seq_1
     misc_feature    1259..1289
                     /label=rBG-pA_seq_2
     CDS             complement(1372..2397)
                     /codon_start=1
                     /label=HygroB
                     /label=ORF frame 3
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1400..1419
                     /label=Hygro.FOR
     primer_bind     complement(2372..2389)
                     /label=Hygro.REV
     promoter        2411..3022
                     /label=RPBSA
                     /note="RPBSA;constitutive promoter;color: #cc0000; 
                     direction: RIGHT"
     promoter        2411..3022
                     /label=RPBSA promoter
                     /note="RPBSA promoter;constitutive promoter;color: #cc0000;
                     direction: RIGHT"
     exon            2411..2420
                     /label=RPL41 exon2
                     /note="RPL41 exon2;color: #cc0000; direction: RIGHT"
     intron          2421..2535
                     /label=RPL41 intron1
                     /note="RPL41 intron1;color: #cc0000; direction: RIGHT"
     primer_bind     complement(2466..2482)
                     /label=RPBSA_seq.FOR
     exon            2536..2588
                     /label=RPL41 exon1
                     /note="RPL41 exon1;color: #cc0000; direction: RIGHT"
     promoter        2588..3022
                     /label=RPL13a Promoter
                     /note="RPL13a Promoter;color: #cc0000; direction: RIGHT"
     misc_feature    2929..2936
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     promoter        3030..4211
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3260..4202
                     /label=EF1a Intron1
                     /note="EF1a Intron1;color: #cc0000; direction: LEFT"
     misc_feature    4159..4179
                     /label=EF1a_fwd_primer
     primer_bind     4163..4183
                     /label=pSB_EF1a_seq.FOR
     exon            4203..4221
                     /label=EF1a Exon2
                     /note="EF1a Exon2;color: #cc0000; direction: LEFT"
     primer_bind     4208..4273
                     /label=pAF137-1.FOR
     misc_feature    4208..4245
                     /label=Gibson_overlap_5'
     misc_feature    4227..4233
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     misc_feature    4234..4241
                     /label=NotI
                     /note="Gene-of-interest (GOI) 5' NotI restriction digest 
                     site"
     misc_feature    4237..4245
                     /label=Kozak
                     /note="
                     
                     
                     
                     
                     
                     
                     
                     Kozak
                     consensus sequence"
     CDS             4246..5619
                     /codon_start=1
                     /label=pAF138_ORF
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQPGENLYFQGSAGSAAGSGEFHHHHHHVDVSK
                     GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT
                     TFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVN
                     RIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLAD
                     HYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             4246..4818
                     /codon_start=1
                     /product="Odds of being secreted ~6 (similar to deIFNg)"
                     /label=sha_TNFRSF9_sigpep-ECD
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     CDS             4246..4818
                     /codon_start=1
                     /label=devil 4-1BB
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     sig_peptide     4246..4320
                     /label=sha_TNFRSF9-201_SigP
     misc_feature    4246..4320
                     /label=signal peptide predicted by SignalIP 4.1
                     /note="signal peptide predicted by SignalIP 4.1"
     CDS             4321..5616
                     /codon_start=1
                     /label=pAF138_ORF_no-SigP
                     /translation="SCGSCPAGTFWNGTDCGKNKNQACIPCPPNSFSSTAGEQRTCNIC
                     RRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGCNRCQPDCKQGQELLGKGCRDCSFGT
                     FNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVICGSTQTVATLDTISMTIPASLVHIDK
                     NHQPGENLYFQGSAGSAAGSGEFHHHHHHVDVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN
                     SHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQS
                     KLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    4321..4818
                     /label=ECD predicted by TMHMM
                     /note="ECD predicted by TMHMM"
     primer_bind     complement(4794..4861)
                     /label=pAF137-1.REV
     misc_feature    4819..4850
                     /label=Gibson_3'_overlap
     misc_feature    4819..4824
                     /label=XmaI
                     /note="XmaI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4825..4845
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4846..4878
                     /codon_start=1
                     /product="Chen, X., et al. 2013. Advanced Drug Delivery 
                     Reviews. Fusion protein linkers: Property, design and 
                     functionality Waldo, G. S., et al. 1999. Nat Biotech. Rapid
                     protein-folding assay using green fluorescent protein 
                     Pedelacq, J.-D., et al. 2006. Nat Biotech. Engineering and 
                     characterization of a superfolder green fluorescent 
                     protein"
                     /label=GSAGSAAGSGEF linker
                     /translation="SAGSAAGSGEF"
     primer_bind     4873..4921
                     /label=pAF138.FOR
     CDS             4879..4896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4897..4902
                     /label=SalI
                     /note="SalI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4903..5616
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002) mCitrinemEYFP (mCitrine) with 
                     A206K mutation for monomer
                     "
                     /label=mEYFP (mCitrine)
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5593..5650)
                     /label=pAF138.REV
     misc_feature    5620..5625
                     /label=NheI
                     /note="NheI restriction digest site"
     misc_feature    5626..5633
                     /label=3'-SfiI
                     /note="3'-SfiI;GGCCTGTCAGGCC;color: #a6acb3"
     polyA_signal    5634..5931
                     /label=bGH-pA
                     /note="bGH-pA;bovine growth hormone polyadenylation 
                     signal;color: #cc0000; direction: LEFT"
     primer_bind     complement(5666..5684)
                     /label=pSB_bGH_seq.REV
     misc_feature    5671..5688
                     /label=BGH_rev_primer
     primer_bind     complement(5671..5688)
                     /label=BGH_rev_primer
     polyA_signal    5677..5901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5942..5983
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    5942..5983
                     /label=5'-proxITR
                     /note="5'-proxITR;modified region proximal to 5'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    5989..6213
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6209..6213
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6245..6263
                     /label=5-ITR_seq_1
     enhancer        6263..6566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6267..6554
                     /label=CAG_enhancer
     promoter        6272..6764
                     /label=CMV_immearly_promoter
     promoter        6567..6770
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6721..6741
                     /label=CMV_fwd_primer
     primer_bind     6721..6741
                     /label=CMV_fwd_primer
     promoter        6722..6791
                     /label=CMV_promoter
     promoter        6734..6853
                     /label=CMV2_promoter
     misc_feature    6765..6784
                     /label=pCEP_fwd_primer
     misc_feature    6767..6791
                     /label=LNCX_primer
     misc_feature    6908..7004
                     /label=modSV40_late_16s_int
     intron          6908..7004
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    6908..6938
                     /label=SV40_late_19s_int
     primer_bind     7061..7080
                     /label=T7.FOR
     promoter        7061..7079
                     /label=T7_promoter
     CDS             7090..8112
                     /codon_start=1
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8129..8263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8153..8172)
                     /label=EBV_rev_primer
     primer_bind     complement(8153..8172)
                     /label=EBV.REV
     primer_bind     complement(8207..8226)
                     /label=SV40_pAn1.REV
     primer_bind     complement(8305..8324)
                     /label=SV40_pAn2.REV, pVitro1R
     misc_feature    8327..8355
                     /label=pSB_seq_1.FOR
     promoter        8416..8520
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
     gene            8521..9381
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             8521..9378
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: 1: 5975 .. 6766 / #ccffcc 2: 6767 .. 6835 / 
                     #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt aaacagctac
     4261 aatagactgg aggcgatatc aactcttgtt atactactct gggtaactct ggtccagggt
     4321 tcctgtggca gctgtccagc tggtactttc tggaatggta cagattgtgg aaaaaacaaa
     4381 aatcaagctt gcatcccatg tcccccaaat agtttctcta gtacagctgg cgaacagagg
     4441 acatgtaaca tatgcaggag atgtgaaggt atttttagaa caaagagaga atgtactcca
     4501 acaagtaata cagagtgtga ttgtatgtct gggtatcatt gttccggagt aggatgtaat
     4561 aggtgtcaac cagattgcaa gcaaggtcaa gaacttctag gaaaaggttg tagagactgt
     4621 agctttggta cctttaatga tcagacacgg gggaactgca aacgctggac aaactgctct
     4681 ttgaatggac tagttgtgct tgtaaatgga accaataaaa gagatgtgat atgtggatcc
     4741 acccaaacag ttgccactct ggacacaatc tccatgacaa ttccagcttc tctcgtgcac
     4801 attgataaga accatcagcc cggggagaac ctttacttcc agggatccgc tggctccgct
     4861 gctggttctg gcgaattcca tcatcaccat caccatgtcg acgtgagcaa gggcgaggag
     4921 ctgttcaccg gcgtggtgcc catcctggtg gagctggacg gcgacgtgaa cggccacaag
     4981 ttcagcgtga gcggcgaggg cgagggcgac gccacctacg gcaagttgac cctgaagttc
     5041 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tggtgaccac cttcggctac
     5101 ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagagc
     5161 gccatgcccg agggctacgt gcaggagcgc accatcttct tcaaggacga cggcaactac
     5221 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5281 ggcatcgact tcaaggagga cggcaacatc ctgggccaca agctggagta caactacaac
     5341 agccacaacg tgtacatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     5401 atccgccaca acatcgagga cggcagcgtg cagctggccg accactacca gcagaacacc
     5461 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagagcaag
     5521 ctgagcaagg accccaacga gaagcgcgac cacatggtgc tgctggagtt cgtgaccgcc
     5581 gccggcatca ccctgggcat ggacgagctg tacaagtagg ctagcgtcag gccaagcttg
     5641 gggatcaatt ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     5701 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5761 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5821 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5881 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctgggcc tactagatag
     5941 cggacccctt accgaaacat cgccgcattc tgcagaggag tcgaacttag tgtatgtaaa
     6001 cttctgaccc actggaattg tgatacagtg aattataagt gaaataatct gtctgtaaac
     6061 aattgttgga aaaatgactt gtgtcatgca caaagtagat gtcctaactg acttgccaaa
     6121 actattgttt gttaacaaga aatttgtgga gtagttgaaa aacgagtttt aatgactcca
     6181 acttaagtgt atgtaaactt ccgacttcaa ctgtataggg atccaatttt aacaaaatat
     6241 tcgagcttgc atgcctgcag gtcgttacat aacttacggt aaatggcccg cctggctgac
     6301 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     6361 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     6421 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     6481 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     6541 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     6601 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     6661 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     6721 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     6781 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     6841 accgatccag cctccggact ctagaggatc cggtactcga ggaactgaaa aaccagaaag
     6901 ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatccg gtggtggtgc
     6961 aaatcaaaga actgctcctc agtggatgtt gcctttactt ctaggcctgt acggaagtgt
     7021 tacttctgct ctaaaagctg cggaattgta cccgcggaat taatacgact cactataggg
     7081 actagtacca tgggaaaatc aaaagaaatc agccaagacc tcagaaaaag aattgtagac
     7141 ctccacaagt ctggttcatc cttgggagca atttccaaac gcctggcggt accacgttca
     7201 tctgtacaaa caatagtacg caagtataaa caccatggga ccacgcagcc gtcataccgc
     7261 tcaggaagga gacgcgttct gtctcctaga gatgaacgta ctttggtgcg aaaagtgcaa
     7321 atcaatccca gaacaacagc aaaggacctt gtgaagatgc tggaggaaac aggtacaaaa
     7381 gtatctatat ccacagtaaa acgagtccta tatcgacata acctgaaagg ccactcagca
     7441 aggaagaagc cactgctcca aaaccgacat aagaaagcca gactacggtt tgcaactgca
     7501 catggggaca aagatcgtac tttttggaga aatgtcctct ggtctgatga aacaaaaata
     7561 gaactgtttg gccataatga ccatcgttat gtttggagga agaaggggga ggcttgcaag
     7621 ccgaagaaca ccatcccaac cgtgaagcac gggggtggca gcatcatgtt gtgggggtgc
     7681 tttgctgcag gagggactgg tgcacttcac aaaatagatg gcatcatgga cgcggtgcag
     7741 tatgtggata tattgaagca acatctcaag acatcagtca ggaagttaaa gcttggtcgc
     7801 aaatgggtct tccaacacga caatgacccc aagcatactt ccaaagttgt ggcaaaatgg
     7861 cttaaggaca acaaagtcaa ggtattggag tggccatcac aaagccctga cctcaatcct
     7921 atagaaaatt tgtgggcaga actgaaaaag cgtgtgcgag caaggaggcc tacaaacctg
     7981 actcagttac accagctctg tcaggaggaa tgggccaaaa ttcacccaaa ttattgtggg
     8041 aagcttgtgg aaggctaccc gaaacgtttg acccaagtta aacaatttaa aggcaatgct
     8101 accaaatact aggggcccta accgcgggga tccagacatg ataagataca ttgatgagtt
     8161 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     8221 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     8281 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaacgct tacaatttcc
     8341 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     8401 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8461 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8521 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8581 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8641 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8701 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8761 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8821 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8881 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8941 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9001 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9061 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9121 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9181 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9241 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9301 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9361 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9421 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9481 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9541 aaaggatctt c
//
