LOCUS       Exported                9536 bp ds-DNA     circular SYN 19-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Andrew S. Flies
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    459..477
                     /label=ori.REV
     misc_feature    777..1001
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1002..1043
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    1054..1354
                     /label=rBG-pA
                     /note="rBG-pA;rabbit beta-globin polyadenylation 
                     signal;color: #cc0000; direction: RIGHT"
     misc_feature    1068..1092
                     /label=rBG-pA_seq_1
     misc_feature    1259..1289
                     /label=rBG-pA_seq_2
     CDS             complement(1372..2397)
                     /codon_start=1
                     /label=HygroB
                     /label=ORF frame 3
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1400..1419
                     /label=Hygro.FOR
     primer_bind     complement(2372..2389)
                     /label=Hygro.REV
     promoter        2411..3022
                     /label=RPBSA promoter
                     /note="RPBSA promoter;constitutive promoter;color: #cc0000;
                     direction: RIGHT"
     promoter        2411..3022
                     /label=RPBSA
                     /note="RPBSA;constitutive promoter;color: #cc0000; 
                     direction: RIGHT"
     exon            2411..2420
                     /label=RPL41 exon2
                     /note="RPL41 exon2;color: #cc0000; direction: RIGHT"
     intron          2421..2535
                     /label=RPL41 intron1
                     /note="RPL41 intron1;color: #cc0000; direction: RIGHT"
     primer_bind     complement(2466..2482)
                     /label=RPBSA_seq.FOR
     exon            2536..2588
                     /label=RPL41 exon1
                     /note="RPL41 exon1;color: #cc0000; direction: RIGHT"
     promoter        2588..3022
                     /label=RPL13a Promoter
                     /note="RPL13a Promoter;color: #cc0000; direction: RIGHT"
     misc_feature    2929..2936
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     promoter        3029..4221
                     /label=EF1a promoter
                     /note="EF1a promoter;constitutive promoter;color: #cc0000; 
                     direction: LEFT"
     intron          3260..4202
                     /label=EF1a Intron1
                     /note="EF1a Intron1;color: #cc0000; direction: LEFT"
     misc_feature    4159..4179
                     /label=EF1a_fwd_primer
     primer_bind     4163..4183
                     /label=pSB_EF1a_seq.FOR
     exon            4203..4221
                     /label=EF1a Exon2
                     /note="EF1a Exon2;color: #cc0000; direction: LEFT"
     primer_bind     4208..4273
                     /label=pAF137-1.FOR
     misc_feature    4208..4245
                     /label=Gibson_overlap_5'
     misc_feature    4227..4233
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     misc_feature    4234..4241
                     /label=NotI
                     /note="Gene-of-interest (GOI) 5' NotI restriction digest 
                     site"
     misc_feature    4237..4245
                     /label=Kozak
                     /note="
                     
                     
                     
                     
                     
                     
                     
                     vertebrate consensus sequence for strong initiation of 
                     translation (Kozak, 1987)"
     CDS             4246..5604
                     /codon_start=1
                     /label=pAF139_ORF
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQPGENLYFQGSAGSAAGSGEFHHHHHHVDMSE
                     LIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSF
                     LYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKI
                     RGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRS
                     KKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN"
     CDS             4246..4818
                     /codon_start=1
                     /label=devil 4-1BB
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     CDS             4246..4818
                     /codon_start=1
                     /product="Odds of being secreted ~6 (similar to deIFNg)"
                     /label=sha_TNFRSF9_sigpep-ECD
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     misc_feature    4246..4320
                     /label=signal peptide predicted by SignalIP 4.1
                     /note="signal peptide predicted by SignalIP 4.1"
     sig_peptide     4246..4320
                     /label=sha_TNFRSF9-201_SigP
     CDS             4321..5601
                     /codon_start=1
                     /label=pAF139_ORF_no-SigP
                     /translation="SCGSCPAGTFWNGTDCGKNKNQACIPCPPNSFSSTAGEQRTCNIC
                     RRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGCNRCQPDCKQGQELLGKGCRDCSFGT
                     FNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVICGSTQTVATLDTISMTIPASLVHIDK
                     NHQPGENLYFQGSAGSAAGSGEFHHHHHHVDMSELIKENMHMKLYMEGTVDNHHFKCTS
                     EGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEG
                     FTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTET
                     LYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEA
                     NNETYVEQHEVAVARYCDLPSKLGHKLN"
     misc_feature    4321..4818
                     /label=ECD predicted by TMHMM
                     /note="ECD predicted by TMHMM"
     primer_bind     complement(4794..4861)
                     /label=pAF137-1.REV
     misc_feature    4819..4850
                     /label=Gibson_3'_overlap
     misc_feature    4819..4824
                     /label=XmaI
                     /note="XmaI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4825..4845
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4846..4878
                     /codon_start=1
                     /product="Chen, X., et al. 2013. Advanced Drug Delivery 
                     Reviews. Fusion protein linkers: Property, design and 
                     functionality Waldo, G. S., et al. 1999. Nat Biotech. Rapid
                     protein-folding assay using green fluorescent protein 
                     Pedelacq, J.-D., et al. 2006. Nat Biotech. Engineering and 
                     characterization of a superfolder green fluorescent 
                     protein"
                     /label=GSAGSAAGSGEF linker
                     /translation="SAGSAAGSGEF"
     CDS             4879..4896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4897..4902
                     /label=SalI
                     /note="SalI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4903..5601
                     /codon_start=1
                     /product="TagBFP (scientific name mTagBFP) is a monomeric 
                     blue fluorescent protein generated by site-specific and 
                     random mutagenesis of TagRFP [Subach et al., 2008]. TagBFP 
                     possesses bright blue fluorescence with excitation/emission
                     maxima at 402 and 457 nm, characterized by high 
                     photostability and extremely high pH-stability."
                     /label=mTag-BFP
                     /note="mTag-BFP;color: #cc0000"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(5579..5635)
                     /label=pAF139.REV
     misc_feature    5605..5610
                     /label=NheI
                     /note="NheI restriction digest site"
     misc_feature    5611..5618
                     /label=3'-SfiI
                     /note="3'-SfiI;GGCCTGTCAGGCC;color: #a6acb3"
     polyA_signal    5619..5916
                     /label=bGH-pA
                     /note="bGH-pA;bovine growth hormone polyadenylation 
                     signal;color: #cc0000; direction: LEFT"
     primer_bind     complement(5651..5669)
                     /label=pSB_bGH_seq.REV
     misc_feature    5656..5673
                     /label=BGH_rev_primer
     primer_bind     complement(5656..5673)
                     /label=BGH_rev_primer
     primer_bind     5656..5673
                     /label=BGH.FOR
     polyA_signal    5662..5886
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5927..5968
                     /label=5'-proxITR
                     /note="5'-proxITR;modified region proximal to 5'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    5974..6198
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6230..6248
                     /label=5-ITR_seq_1
     enhancer        6248..6551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6252..6539
                     /label=CAG_enhancer
     promoter        6257..6749
                     /label=CMV_immearly_promoter
     promoter        6552..6755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6706..6726
                     /label=CMV_fwd_primer
     primer_bind     6706..6726
                     /label=CMV_fwd_primer
     promoter        6707..6776
                     /label=CMV_promoter
     promoter        6719..6838
                     /label=CMV2_promoter
     misc_feature    6750..6769
                     /label=pCEP_fwd_primer
     misc_feature    6752..6776
                     /label=LNCX_primer
     intron          6893..6989
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     intron          6893..6989
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    6893..6989
                     /label=modSV40_late_16s_int
     misc_feature    6893..6923
                     /label=SV40_late_19s_int
     primer_bind     7046..7065
                     /label=T7.FOR
     promoter        7046..7064
                     /label=T7_promoter
     CDS             7075..8097
                     /codon_start=1
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8114..8248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8138..8157)
                     /label=EBV_rev_primer
     primer_bind     complement(8138..8157)
                     /label=EBV.REV
     primer_bind     complement(8192..8211)
                     /label=SV40_pAn1.REV
     primer_bind     complement(8290..8309)
                     /label=SV40_pAn2.REV, pVitro1R
     misc_feature    8312..8340
                     /label=pSB_seq_1.FOR
     promoter        8401..8505
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
     gene            8506..9366
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             8506..9363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: 1: 5975 .. 6766 / #ccffcc 2: 6767 .. 6835 / 
                     #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt aaacagctac
     4261 aatagactgg aggcgatatc aactcttgtt atactactct gggtaactct ggtccagggt
     4321 tcctgtggca gctgtccagc tggtactttc tggaatggta cagattgtgg aaaaaacaaa
     4381 aatcaagctt gcatcccatg tcccccaaat agtttctcta gtacagctgg cgaacagagg
     4441 acatgtaaca tatgcaggag atgtgaaggt atttttagaa caaagagaga atgtactcca
     4501 acaagtaata cagagtgtga ttgtatgtct gggtatcatt gttccggagt aggatgtaat
     4561 aggtgtcaac cagattgcaa gcaaggtcaa gaacttctag gaaaaggttg tagagactgt
     4621 agctttggta cctttaatga tcagacacgg gggaactgca aacgctggac aaactgctct
     4681 ttgaatggac tagttgtgct tgtaaatgga accaataaaa gagatgtgat atgtggatcc
     4741 acccaaacag ttgccactct ggacacaatc tccatgacaa ttccagcttc tctcgtgcac
     4801 attgataaga accatcagcc cggggagaac ctttacttcc agggatccgc tggctccgct
     4861 gctggttctg gcgaattcca tcatcaccat caccatgtcg acatgagcga gctgattaag
     4921 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     4981 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     5041 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     5101 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     5161 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     5221 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     5281 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     5341 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     5401 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     5461 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     5521 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     5581 aaactggggc acaagctgaa ctaggctagc gtcaggccaa gcttggggat caattctcta
     5641 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5701 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5761 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5821 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5881 ctatggcttc tgaggcggaa agaaccagct gggcctacta gatagcggac cccttaccga
     5941 aacatcgccg cattctgcag aggagtcgaa cttagtgtat gtaaacttct gacccactgg
     6001 aattgtgata cagtgaatta taagtgaaat aatctgtctg taaacaattg ttggaaaaat
     6061 gacttgtgtc atgcacaaag tagatgtcct aactgacttg ccaaaactat tgtttgttaa
     6121 caagaaattt gtggagtagt tgaaaaacga gttttaatga ctccaactta agtgtatgta
     6181 aacttccgac ttcaactgta tagggatcca attttaacaa aatattcgag cttgcatgcc
     6241 tgcaggtcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     6301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     6361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     6421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     6481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     6541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     6601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     6661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     6721 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     6781 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     6841 ggactctaga ggatccggta ctcgaggaac tgaaaaacca gaaagttaac tggtaagttt
     6901 agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc aaagaactgc
     6961 tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt ctgctctaaa
     7021 agctgcggaa ttgtacccgc ggaattaata cgactcacta tagggactag taccatggga
     7081 aaatcaaaag aaatcagcca agacctcaga aaaagaattg tagacctcca caagtctggt
     7141 tcatccttgg gagcaatttc caaacgcctg gcggtaccac gttcatctgt acaaacaata
     7201 gtacgcaagt ataaacacca tgggaccacg cagccgtcat accgctcagg aaggagacgc
     7261 gttctgtctc ctagagatga acgtactttg gtgcgaaaag tgcaaatcaa tcccagaaca
     7321 acagcaaagg accttgtgaa gatgctggag gaaacaggta caaaagtatc tatatccaca
     7381 gtaaaacgag tcctatatcg acataacctg aaaggccact cagcaaggaa gaagccactg
     7441 ctccaaaacc gacataagaa agccagacta cggtttgcaa ctgcacatgg ggacaaagat
     7501 cgtacttttt ggagaaatgt cctctggtct gatgaaacaa aaatagaact gtttggccat
     7561 aatgaccatc gttatgtttg gaggaagaag ggggaggctt gcaagccgaa gaacaccatc
     7621 ccaaccgtga agcacggggg tggcagcatc atgttgtggg ggtgctttgc tgcaggaggg
     7681 actggtgcac ttcacaaaat agatggcatc atggacgcgg tgcagtatgt ggatatattg
     7741 aagcaacatc tcaagacatc agtcaggaag ttaaagcttg gtcgcaaatg ggtcttccaa
     7801 cacgacaatg accccaagca tacttccaaa gttgtggcaa aatggcttaa ggacaacaaa
     7861 gtcaaggtat tggagtggcc atcacaaagc cctgacctca atcctataga aaatttgtgg
     7921 gcagaactga aaaagcgtgt gcgagcaagg aggcctacaa acctgactca gttacaccag
     7981 ctctgtcagg aggaatgggc caaaattcac ccaaattatt gtgggaagct tgtggaaggc
     8041 tacccgaaac gtttgaccca agttaaacaa tttaaaggca atgctaccaa atactagggg
     8101 ccctaaccgc ggggatccag acatgataag atacattgat gagtttggac aaaccacaac
     8161 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     8221 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     8281 ggttcagggg gaggtgtggg aggtttttta acgcttacaa tttcctgatg cggtattttc
     8341 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
     8401 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8461 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8521 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8581 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8641 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8701 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8761 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8821 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8881 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8941 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9001 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9061 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9121 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9181 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9241 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9301 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9361 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9421 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9481 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
