LOCUS       Exported                9566 bp ds-DNA     circular SYN 05-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Andrew S. Flies
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4903..5631
                     /organism="mNeptune2-N1 Sequencing Result"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    777..1001
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1002..1043
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    1054..1354
                     /label=rBG-pA
                     /note="rBG-pA;rabbit beta-globin polyadenylation 
                     signal;color: #cc0000; direction: RIGHT"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /label=HygroB
                     /label=ORF frame 3
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1400..1419
                     /label=Hygro.FOR
     primer_bind     complement(2372..2389)
                     /label=Hygro.REV
     promoter        2411..3022
                     /label=RPBSA
                     /note="RPBSA;constitutive promoter;color: #cc0000; 
                     direction: RIGHT"
     promoter        2411..3022
                     /label=RPBSA promoter
                     /note="RPBSA promoter;constitutive promoter;color: #cc0000;
                     direction: RIGHT"
     exon            2411..2420
                     /label=RPL41 exon2
                     /note="RPL41 exon2;color: #cc0000; direction: RIGHT"
     intron          2421..2535
                     /label=RPL41 intron1
                     /note="RPL41 intron1;color: #cc0000; direction: RIGHT"
     primer_bind     complement(2466..2482)
                     /label=RPBSA_seq.FOR
     exon            2536..2588
                     /label=RPL41 exon1
                     /note="RPL41 exon1;color: #cc0000; direction: RIGHT"
     promoter        2588..3022
                     /label=RPL13a Promoter
                     /note="RPL13a Promoter;color: #cc0000; direction: RIGHT"
     misc_feature    2929..2936
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     promoter        3029..4221
                     /label=EF1a promoter
                     /note="EF1a promoter;constitutive promoter;color: #cc0000; 
                     direction: LEFT"
     intron          3260..4202
                     /label=EF1a Intron1
                     /note="EF1a Intron1;color: #cc0000; direction: LEFT"
     misc_feature    4159..4179
                     /label=EF1a_fwd_primer
     primer_bind     4163..4183
                     /label=pSB_EF1a_seq.FOR
     exon            4203..4221
                     /label=EF1a Exon2
                     /note="EF1a Exon2;color: #cc0000; direction: LEFT"
     primer_bind     4208..4273
                     /label=pAF137-1.FOR
     misc_feature    4208..4245
                     /label=Gibson_overlap_5'
     misc_feature    4227..4233
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     misc_feature    4234..4241
                     /label=NotI
                     /note="Gene-of-interest (GOI) 5' NotI restriction digest 
                     site"
     misc_feature    4237..4245
                     /label=Kozak
                     /note="
                     
                     
                     
                     
                     
                     
                     
                     Kozak
                     consensus sequence"
     CDS             4246..5634
                     /codon_start=1
                     /label=pAF163_ORF
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQPGENLYFQGSAGSAAGSGEFHHHHHHVDVSK
                     GEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGRIKVVEGGPLPFAFDILA
                     TCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTVTQDTSLQDGCLIYN
                     VKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTT
                     YRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMD
                     ELYK"
     CDS             4246..4818
                     /codon_start=1
                     /product="Odds of being secreted ~6 (similar to deIFNg)"
                     /label=sha_TNFRSF9_sigpep-ECD
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     CDS             4246..4818
                     /codon_start=1
                     /label=devil 4-1BB
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     misc_feature    4246..4320
                     /label=signal peptide predicted by SignalIP 4.1
                     /note="signal peptide predicted by SignalIP 4.1"
     misc_feature    4321..4818
                     /label=ECD predicted by TMHMM
                     /note="ECD predicted by TMHMM"
     primer_bind     complement(4794..4861)
                     /label=pAF137-1.REV
     misc_feature    4819..4850
                     /label=Gibson_3'_overlap
     misc_feature    4819..4824
                     /label=XmaI
                     /note="XmaI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4825..4845
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4846..4878
                     /codon_start=1
                     /product="Chen, X., et al. 2013. Advanced Drug Delivery 
                     Reviews. Fusion protein linkers: Property, design and 
                     functionality Waldo, G. S., et al. 1999. Nat Biotech. Rapid
                     protein-folding assay using green fluorescent protein 
                     Pedelacq, J.-D., et al. 2006. Nat Biotech. Engineering and 
                     characterization of a superfolder green fluorescent 
                     protein"
                     /label=GSAGSAAGSGEF linker
                     /translation="SAGSAAGSGEF"
     primer_bind     4873..4923
                     /label=pAF163.FOR
     CDS             4879..4896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4897..4902
                     /label=SalI
                     /note="SalI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4903..5631
                     /codon_start=1
                     /product="#7cb7bb"
                     /label=mNeptune2_54837
                     /label=ORF frame 1
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTGR
                     IKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCDM
                     ALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     misc_feature    5635..5640
                     /label=NheI
                     /note="NheI restriction digest site"
     misc_feature    5641..5648
                     /label=3'-SfiI
                     /note="3'-SfiI;GGCCTGTCAGGCC;color: #a6acb3"
     polyA_signal    5649..5946
                     /label=bGH-pA
                     /note="bGH-pA;bovine growth hormone polyadenylation 
                     signal;color: #cc0000; direction: LEFT"
     primer_bind     complement(5681..5699)
                     /label=pSB_bGH_seq.REV
     misc_feature    5686..5703
                     /label=BGH_rev_primer
     primer_bind     complement(5686..5703)
                     /label=BGH_rev_primer
     primer_bind     5686..5703
                     /label=BGH.FOR
     polyA_signal    5692..5916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5957..5998
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    5957..5998
                     /label=5'-proxITR
                     /note="5'-proxITR;modified region proximal to 5'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    6004..6228
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6224..6228
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     enhancer        6278..6581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6282..6569
                     /label=CAG_enhancer
     promoter        6287..6779
                     /label=CMV_immearly_promoter
     promoter        6582..6785
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6736..6756
                     /label=CMV_fwd_primer
     primer_bind     6736..6756
                     /label=CMV_fwd_primer
     promoter        6737..6806
                     /label=CMV_promoter
     promoter        6749..6868
                     /label=CMV2_promoter
     misc_feature    6780..6799
                     /label=pCEP_fwd_primer
     misc_feature    6782..6806
                     /label=LNCX_primer
     misc_feature    6923..7019
                     /label=modSV40_late_16s_int
     intron          6923..7019
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     intron          6923..7019
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    6923..6953
                     /label=SV40_late_19s_int
     primer_bind     7076..7095
                     /label=T7.FOR
     promoter        7076..7094
                     /label=T7_promoter
     CDS             7105..8127
                     /codon_start=1
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8144..8278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8168..8187)
                     /label=EBV_rev_primer
     primer_bind     complement(8168..8187)
                     /label=EBV.REV
     primer_bind     complement(8222..8241)
                     /label=SV40_pAn1.REV
     primer_bind     complement(8320..8339)
                     /label=SV40_pAn2.REV, pVitro1R
     promoter        8431..8535
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
     gene            8536..9396
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             8536..9393
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: 1: 5975 .. 6766 / #ccffcc 2: 6767 .. 6835 / 
                     #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt aaacagctac
     4261 aatagactgg aggcgatatc aactcttgtt atactactct gggtaactct ggtccagggt
     4321 tcctgtggca gctgtccagc tggtactttc tggaatggta cagattgtgg aaaaaacaaa
     4381 aatcaagctt gcatcccatg tcccccaaat agtttctcta gtacagctgg cgaacagagg
     4441 acatgtaaca tatgcaggag atgtgaaggt atttttagaa caaagagaga atgtactcca
     4501 acaagtaata cagagtgtga ttgtatgtct gggtatcatt gttccggagt aggatgtaat
     4561 aggtgtcaac cagattgcaa gcaaggtcaa gaacttctag gaaaaggttg tagagactgt
     4621 agctttggta cctttaatga tcagacacgg gggaactgca aacgctggac aaactgctct
     4681 ttgaatggac tagttgtgct tgtaaatgga accaataaaa gagatgtgat atgtggatcc
     4741 acccaaacag ttgccactct ggacacaatc tccatgacaa ttccagcttc tctcgtgcac
     4801 attgataaga accatcagcc cggggagaac ctttacttcc agggatccgc tggctccgct
     4861 gctggttctg gcgaattcca tcatcaccat caccatgtcg acgtgtctaa gggcgaagag
     4921 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     4981 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac cggcagaatc
     5041 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac ctgcttcatg
     5101 tacggcagca agaccttcat caaccacacc cagggcatcc ccgatttctt taagcagtcc
     5161 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgcttacc
     5221 gttacccagg acaccagcct ccaggacggc tgcttgatct acaacgtcaa gctcagaggg
     5281 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagt
     5341 accgagaccc tgtaccccgc tgacggcggc ctggaaggca gatgcgacat ggccctgaag
     5401 ctcgtgggcg ggggccacct gcactgcaac ctgaagacca catacagatc caagaaaccc
     5461 gctaagaacc tcaagatgcc cggcgtctac tttgtggacc gcagactgga aagaatcaag
     5521 gaggccgaca atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     5581 ctccctagca aactggggca caaacttaat ggcatggacg agctgtacaa gtaggctagc
     5641 gtcaggccaa gcttggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     5701 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5761 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5821 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5881 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5941 gggcctacta gatagcggac cccttaccga aacatcgccg cattctgcag aggagtcgaa
     6001 cttagtgtat gtaaacttct gacccactgg aattgtgata cagtgaatta taagtgaaat
     6061 aatctgtctg taaacaattg ttggaaaaat gacttgtgtc atgcacaaag tagatgtcct
     6121 aactgacttg ccaaaactat tgtttgttaa caagaaattt gtggagtagt tgaaaaacga
     6181 gttttaatga ctccaactta agtgtatgta aacttccgac ttcaactgta tagggatcca
     6241 attttaacaa aatattcgag cttgcatgcc tgcaggtcgt tacataactt acggtaaatg
     6301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     6361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     6421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     6481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     6541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     6601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     6661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     6721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     6781 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     6841 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatccggta ctcgaggaac
     6901 tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt caggtcccgg
     6961 atccggtggt ggtgcaaatc aaagaactgc tcctcagtgg atgttgcctt tacttctagg
     7021 cctgtacgga agtgttactt ctgctctaaa agctgcggaa ttgtacccgc ggaattaata
     7081 cgactcacta tagggactag taccatggga aaatcaaaag aaatcagcca agacctcaga
     7141 aaaagaattg tagacctcca caagtctggt tcatccttgg gagcaatttc caaacgcctg
     7201 gcggtaccac gttcatctgt acaaacaata gtacgcaagt ataaacacca tgggaccacg
     7261 cagccgtcat accgctcagg aaggagacgc gttctgtctc ctagagatga acgtactttg
     7321 gtgcgaaaag tgcaaatcaa tcccagaaca acagcaaagg accttgtgaa gatgctggag
     7381 gaaacaggta caaaagtatc tatatccaca gtaaaacgag tcctatatcg acataacctg
     7441 aaaggccact cagcaaggaa gaagccactg ctccaaaacc gacataagaa agccagacta
     7501 cggtttgcaa ctgcacatgg ggacaaagat cgtacttttt ggagaaatgt cctctggtct
     7561 gatgaaacaa aaatagaact gtttggccat aatgaccatc gttatgtttg gaggaagaag
     7621 ggggaggctt gcaagccgaa gaacaccatc ccaaccgtga agcacggggg tggcagcatc
     7681 atgttgtggg ggtgctttgc tgcaggaggg actggtgcac ttcacaaaat agatggcatc
     7741 atggacgcgg tgcagtatgt ggatatattg aagcaacatc tcaagacatc agtcaggaag
     7801 ttaaagcttg gtcgcaaatg ggtcttccaa cacgacaatg accccaagca tacttccaaa
     7861 gttgtggcaa aatggcttaa ggacaacaaa gtcaaggtat tggagtggcc atcacaaagc
     7921 cctgacctca atcctataga aaatttgtgg gcagaactga aaaagcgtgt gcgagcaagg
     7981 aggcctacaa acctgactca gttacaccag ctctgtcagg aggaatgggc caaaattcac
     8041 ccaaattatt gtgggaagct tgtggaaggc tacccgaaac gtttgaccca agttaaacaa
     8101 tttaaaggca atgctaccaa atactagggg ccctaaccgc ggggatccag acatgataag
     8161 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     8221 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     8281 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     8341 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8401 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8461 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8521 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8581 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8641 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8701 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8761 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8821 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8881 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8941 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9001 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9061 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9121 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9181 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9241 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9301 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9361 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9421 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9481 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9541 cccgtagaaa agatcaaagg atcttc
//
