LOCUS       Exported                9542 bp ds-DNA     circular SYN 19-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Andrew S. Flies
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4903..5607
                     /organism="mOrange-N1 Sequencing Result"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    459..477
                     /label=ori.REV
     misc_feature    777..1001
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1002..1043
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    1054..1354
                     /label=rBG-pA
                     /note="rBG-pA;rabbit beta-globin polyadenylation 
                     signal;color: #cc0000; direction: RIGHT"
     misc_feature    1068..1092
                     /label=rBG-pA_seq_1
     CDS             complement(1372..2397)
                     /codon_start=1
                     /label=HygroB
                     /label=ORF frame 3
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1400..1419
                     /label=Hygro.FOR
     primer_bind     complement(2372..2389)
                     /label=Hygro.REV
     promoter        2411..3022
                     /label=RPBSA promoter
                     /note="RPBSA promoter;constitutive promoter;color: #cc0000;
                     direction: RIGHT"
     promoter        2411..3022
                     /label=RPBSA
                     /note="RPBSA;constitutive promoter;color: #cc0000; 
                     direction: RIGHT"
     exon            2411..2420
                     /label=RPL41 exon2
                     /note="RPL41 exon2;color: #cc0000; direction: RIGHT"
     intron          2421..2535
                     /label=RPL41 intron1
                     /note="RPL41 intron1;color: #cc0000; direction: RIGHT"
     primer_bind     complement(2466..2482)
                     /label=RPBSA_seq.FOR
     exon            2536..2588
                     /label=RPL41 exon1
                     /note="RPL41 exon1;color: #cc0000; direction: RIGHT"
     promoter        2588..3022
                     /label=RPL13a Promoter
                     /note="RPL13a Promoter;color: #cc0000; direction: RIGHT"
     misc_feature    2929..2936
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     promoter        3029..4221
                     /label=EF1a promoter
                     /note="EF1a promoter;constitutive promoter;color: #cc0000; 
                     direction: LEFT"
     intron          3260..4202
                     /label=EF1a Intron1
                     /note="EF1a Intron1;color: #cc0000; direction: LEFT"
     misc_feature    4159..4179
                     /label=EF1a_fwd_primer
     primer_bind     4163..4183
                     /label=pSB_EF1a_seq.FOR
     exon            4203..4221
                     /label=EF1a Exon2
                     /note="EF1a Exon2;color: #cc0000; direction: LEFT"
     primer_bind     4208..4273
                     /label=pAF137-1.FOR
     misc_feature    4208..4245
                     /label=Gibson_overlap_5'
     misc_feature    4227..4233
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     misc_feature    4234..4241
                     /label=NotI
                     /note="Gene-of-interest (GOI) 5' NotI restriction digest 
                     site"
     misc_feature    4237..4245
                     /label=Kozak
                     /note="
                     
                     
                     
                     
                     
                     
                     
                     Kozak
                     consensus sequence"
     CDS             4246..5610
                     /codon_start=1
                     /label=pAF164_ORF
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQPGENLYFQGSAGSAAGSGEFHHHHHHVDVSK
                     GEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGFQTAKLKVTKGGPLPFAW
                     DILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGE
                     FIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKMRLKLKDGGHYTSE
                     VKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     CDS             4246..4818
                     /codon_start=1
                     /label=devil 4-1BB
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     CDS             4246..4818
                     /codon_start=1
                     /product="Odds of being secreted ~6 (similar to deIFNg)"
                     /label=sha_TNFRSF9_sigpep-ECD
                     /translation="MVNSYNRLEAISTLVILLWVTLVQGSCGSCPAGTFWNGTDCGKNK
                     NQACIPCPPNSFSSTAGEQRTCNICRRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGC
                     NRCQPDCKQGQELLGKGCRDCSFGTFNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVIC
                     GSTQTVATLDTISMTIPASLVHIDKNHQ"
     sig_peptide     4246..4320
                     /label=sha_TNFRSF9-201_SigP
     misc_feature    4246..4320
                     /label=signal peptide predicted by SignalIP 4.1
                     /note="signal peptide predicted by SignalIP 4.1"
     CDS             4321..5607
                     /codon_start=1
                     /label=pAF164_ORF_no-SigP
                     /translation="SCGSCPAGTFWNGTDCGKNKNQACIPCPPNSFSSTAGEQRTCNIC
                     RRCEGIFRTKRECTPTSNTECDCMSGYHCSGVGCNRCQPDCKQGQELLGKGCRDCSFGT
                     FNDQTRGNCKRWTNCSLNGLVVLVNGTNKRDVICGSTQTVATLDTISMTIPASLVHIDK
                     NHQPGENLYFQGSAGSAAGSGEFHHHHHHVDVSKGEENNMAIIKEFMRFKVRMEGSVNG
                     HEFEIEGEGEGRPYEGFQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYF
                     KLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMG
                     WEASSERMYPEDGALKGEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDI
                     TSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     misc_feature    4321..4818
                     /label=ECD predicted by TMHMM
                     /note="ECD predicted by TMHMM"
     primer_bind     complement(4794..4861)
                     /label=pAF137-1.REV
     misc_feature    4819..4850
                     /label=Gibson_3'_overlap
     misc_feature    4819..4824
                     /label=XmaI
                     /note="XmaI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4825..4845
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4846..4878
                     /codon_start=1
                     /product="Chen, X., et al. 2013. Advanced Drug Delivery 
                     Reviews. Fusion protein linkers: Property, design and 
                     functionality Waldo, G. S., et al. 1999. Nat Biotech. Rapid
                     protein-folding assay using green fluorescent protein 
                     Pedelacq, J.-D., et al. 2006. Nat Biotech. Engineering and 
                     characterization of a superfolder green fluorescent 
                     protein"
                     /label=GSAGSAAGSGEF linker
                     /translation="SAGSAAGSGEF"
     CDS             4879..4896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4897..4902
                     /label=SalI
                     /note="SalI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             4903..5610
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    5611..5616
                     /label=NheI
                     /note="NheI restriction digest site"
     misc_feature    5617..5624
                     /label=3'-SfiI
                     /note="3'-SfiI;GGCCTGTCAGGCC;color: #a6acb3"
     polyA_signal    5625..5922
                     /label=bGH-pA
                     /note="bGH-pA;bovine growth hormone polyadenylation 
                     signal;color: #cc0000; direction: LEFT"
     primer_bind     complement(5657..5675)
                     /label=pSB_bGH_seq.REV
     misc_feature    5662..5679
                     /label=BGH_rev_primer
     primer_bind     complement(5662..5679)
                     /label=BGH_rev_primer
     primer_bind     5662..5679
                     /label=BGH.FOR
     polyA_signal    5668..5892
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5933..5974
                     /label=5'-proxITR
                     /note="5'-proxITR;modified region proximal to 5'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    5980..6204
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6236..6254
                     /label=5-ITR_seq_1
     enhancer        6254..6557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        6254..6557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6263..6755
                     /label=CMV_immearly_promoter
     promoter        6558..6761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6712..6732
                     /label=CMV_fwd_primer
     primer_bind     6712..6732
                     /label=CMV_fwd_primer
     promoter        6713..6782
                     /label=CMV_promoter
     promoter        6725..6844
                     /label=CMV2_promoter
     misc_feature    6756..6775
                     /label=pCEP_fwd_primer
     misc_feature    6758..6782
                     /label=LNCX_primer
     intron          6899..6995
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    6899..6995
                     /label=modSV40_late_16s_int
     misc_feature    6899..6929
                     /label=SV40_late_19s_int
     primer_bind     7052..7071
                     /label=T7.FOR
     promoter        7052..7070
                     /label=T7_promoter
     CDS             7081..8103
                     /codon_start=1
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8120..8254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8144..8163)
                     /label=EBV_rev_primer
     primer_bind     complement(8144..8163)
                     /label=EBV.REV
     primer_bind     complement(8198..8217)
                     /label=SV40_pAn1.REV
     primer_bind     complement(8296..8315)
                     /label=SV40_pAn2.REV, pVitro1R
     misc_feature    8318..8346
                     /label=pSB_seq_1.FOR
     promoter        8407..8511
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
     gene            8512..9372
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             8512..9369
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: 1: 5975 .. 6766 / #ccffcc 2: 6767 .. 6835 / 
                     #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatggt aaacagctac
     4261 aatagactgg aggcgatatc aactcttgtt atactactct gggtaactct ggtccagggt
     4321 tcctgtggca gctgtccagc tggtactttc tggaatggta cagattgtgg aaaaaacaaa
     4381 aatcaagctt gcatcccatg tcccccaaat agtttctcta gtacagctgg cgaacagagg
     4441 acatgtaaca tatgcaggag atgtgaaggt atttttagaa caaagagaga atgtactcca
     4501 acaagtaata cagagtgtga ttgtatgtct gggtatcatt gttccggagt aggatgtaat
     4561 aggtgtcaac cagattgcaa gcaaggtcaa gaacttctag gaaaaggttg tagagactgt
     4621 agctttggta cctttaatga tcagacacgg gggaactgca aacgctggac aaactgctct
     4681 ttgaatggac tagttgtgct tgtaaatgga accaataaaa gagatgtgat atgtggatcc
     4741 acccaaacag ttgccactct ggacacaatc tccatgacaa ttccagcttc tctcgtgcac
     4801 attgataaga accatcagcc cggggagaac ctttacttcc agggatccgc tggctccgct
     4861 gctggttctg gcgaattcca tcatcaccat caccatgtcg acgtgagcaa gggcgaggag
     4921 aataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     4981 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggctttcag
     5041 accgctaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     5101 cctcagttca cctacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     5161 ttcaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5221 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     5281 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     5341 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     5401 atgaggctga agctgaagga cggcggccac tacacctccg aggtcaagac cacctacaag
     5461 gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg gcatcaagtt ggacatcacc
     5521 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     5581 accggcggca tggacgagct gtacaagtag gctagcgtca ggccaagctt ggggatcaat
     5641 tctctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5701 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5761 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5821 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5881 tgggctctat ggcttctgag gcggaaagaa ccagctgggc ctactagata gcggacccct
     5941 taccgaaaca tcgccgcatt ctgcagagga gtcgaactta gtgtatgtaa acttctgacc
     6001 cactggaatt gtgatacagt gaattataag tgaaataatc tgtctgtaaa caattgttgg
     6061 aaaaatgact tgtgtcatgc acaaagtaga tgtcctaact gacttgccaa aactattgtt
     6121 tgttaacaag aaatttgtgg agtagttgaa aaacgagttt taatgactcc aacttaagtg
     6181 tatgtaaact tccgacttca actgtatagg gatccaattt taacaaaata ttcgagcttg
     6241 catgcctgca ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     6301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     6361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6541 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6661 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     6721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     6781 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     6841 gcctccggac tctagaggat ccggtactcg aggaactgaa aaaccagaaa gttaactggt
     6901 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
     6961 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
     7021 tctaaaagct gcggaattgt acccgcggaa ttaatacgac tcactatagg gactagtacc
     7081 atgggaaaat caaaagaaat cagccaagac ctcagaaaaa gaattgtaga cctccacaag
     7141 tctggttcat ccttgggagc aatttccaaa cgcctggcgg taccacgttc atctgtacaa
     7201 acaatagtac gcaagtataa acaccatggg accacgcagc cgtcataccg ctcaggaagg
     7261 agacgcgttc tgtctcctag agatgaacgt actttggtgc gaaaagtgca aatcaatccc
     7321 agaacaacag caaaggacct tgtgaagatg ctggaggaaa caggtacaaa agtatctata
     7381 tccacagtaa aacgagtcct atatcgacat aacctgaaag gccactcagc aaggaagaag
     7441 ccactgctcc aaaaccgaca taagaaagcc agactacggt ttgcaactgc acatggggac
     7501 aaagatcgta ctttttggag aaatgtcctc tggtctgatg aaacaaaaat agaactgttt
     7561 ggccataatg accatcgtta tgtttggagg aagaaggggg aggcttgcaa gccgaagaac
     7621 accatcccaa ccgtgaagca cgggggtggc agcatcatgt tgtgggggtg ctttgctgca
     7681 ggagggactg gtgcacttca caaaatagat ggcatcatgg acgcggtgca gtatgtggat
     7741 atattgaagc aacatctcaa gacatcagtc aggaagttaa agcttggtcg caaatgggtc
     7801 ttccaacacg acaatgaccc caagcatact tccaaagttg tggcaaaatg gcttaaggac
     7861 aacaaagtca aggtattgga gtggccatca caaagccctg acctcaatcc tatagaaaat
     7921 ttgtgggcag aactgaaaaa gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta
     7981 caccagctct gtcaggagga atgggccaaa attcacccaa attattgtgg gaagcttgtg
     8041 gaaggctacc cgaaacgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac
     8101 taggggccct aaccgcgggg atccagacat gataagatac attgatgagt ttggacaaac
     8161 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     8221 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     8281 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc ttacaatttc ctgatgcggt
     8341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     8401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9541 tc
//
