LOCUS       Exported                9600 bp ds-DNA     circular SYN 19-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Andrew S. Flies
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4246..4311
                     /organism="Mesocricetus auratus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10036"
     source          5107..5658
                     /organism="Sarcophilus harrisii"
                     /chromosome="2"
                     /map="unlocalized"
                     /isolate="Salem"
                     /mol_type="mRNA"
                     /country="Australia: Taronga Zoo, Sydney"
                     /sex="female"
                     /db_xref="taxon:9305"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    777..1001
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1002..1043
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    1054..1354
                     /label=rBG-pA
                     /note="rBG-pA;rabbit beta-globin polyadenylation 
                     signal;color: #cc0000; direction: RIGHT"
     CDS             complement(1372..2397)
                     /codon_start=1
                     /label=HygroB
                     /label=ORF frame 3
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     1400..1419
                     /label=Hygro.FOR
     primer_bind     complement(2372..2389)
                     /label=Hygro.REV
     promoter        2411..3022
                     /label=RPBSA
                     /note="RPBSA;constitutive promoter;color: #cc0000; 
                     direction: RIGHT"
     promoter        2411..3022
                     /label=RPBSA promoter
                     /note="RPBSA promoter;constitutive promoter;color: #cc0000;
                     direction: RIGHT"
     exon            2411..2420
                     /label=RPL41 exon2
                     /note="RPL41 exon2;color: #cc0000; direction: RIGHT"
     intron          2421..2535
                     /label=RPL41 intron1
                     /note="RPL41 intron1;color: #cc0000; direction: RIGHT"
     primer_bind     complement(2466..2482)
                     /label=RPBSA_seq.FOR
     exon            2536..2588
                     /label=RPL41 exon1
                     /note="RPL41 exon1;color: #cc0000; direction: RIGHT"
     promoter        2588..3022
                     /label=RPL13a Promoter
                     /note="RPL13a Promoter;color: #cc0000; direction: RIGHT"
     misc_feature    2929..2936
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     promoter        3029..4221
                     /label=EF1a promoter
                     /note="EF1a promoter;constitutive promoter;color: #cc0000; 
                     direction: LEFT"
     intron          3260..4202
                     /label=EF1a Intron1
                     /note="EF1a Intron1;color: #cc0000; direction: LEFT"
     misc_feature    4159..4179
                     /label=EF1a_fwd_primer
     primer_bind     4163..4183
                     /label=pSB_EF1a_seq.FOR
     exon            4203..4221
                     /label=EF1a Exon2
                     /note="EF1a Exon2;color: #cc0000; direction: LEFT"
     misc_feature    4215..4245
                     /label=Gibson_overlap_5'_insert1
     misc_feature    4227..4239
                     /label=5'-SfiI
                     /note="5'-SfiI;GGCCTCTGAGGCC;color: #a6acb3"
     misc_feature    4234..4241
                     /label=NotI
                     /note="Gene-of-interest (GOI) 5' NotI restriction digest 
                     site"
     misc_feature    4237..4245
                     /label=Kozak
                     /note="
                     
                     
                     
                     
                     
                     
                     
                     Kozak
                     consensus sequence"
     CDS             4246..5659
                     /codon_start=1
                     /label=pAF92_ORF
                     /translation="MYSMQLASCLALTLALLVSSAPVDVSKGEEDNMAIIKEFMRFKVH
                     MEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHP
                     ADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPV
                     MQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYN
                     VNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKASHHHHHHSAGSAAGSGEFENL
                     YFQGPGRANLAHPHAAEGLSAPQFPAQSPYAQLVMKDALVQNQTLSWHNDPHLSRVFLD
                     PRMNYDADKGELEVKVAGLYFVYTHLKLQQVVVPAYSQGTVEVFVTVVQDFGQRAVLNL
                     SLDVGPSSTSTITTSESSLIFLHPGERLRLSMSATAGDFLDWQLVSGANTFGLFWVAGG
                     QPLTDSGTLTVIN"
     CDS             4246..4311
                     /codon_start=1
                     /gene="Il2"
                     /gene_synonym="IL-2"
                     /product="interleukin-2 precursor"
                     /label=mau_IL2_NM_001281629
                     /db_xref="GeneID:101835410"
                     /protein_id="NP_001268558.1"
                     /translation="MYSMQLASCLALTLALLVSSAP"
     sig_peptide     4246..4305
                     /gene="Il2"
                     /gene_synonym="IL-2"
                     /label=mau_IL2_NM_001281629_SigP
                     /inference="COORDINATES:same species:SignalP"
     CDS             4306..5658
                     /codon_start=1
                     /label=pAF92.3_ORF_no-SigP
                     /translation="APVDVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRP
                     YEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQY
                     ERAEGRHSTGGMDELYKASHHHHHHSAGSAAGSGEFENLYFQGPGRANLAHPHAAEGLS
                     APQFPAQSPYAQLVMKDALVQNQTLSWHNDPHLSRVFLDPRMNYDADKGELEVKVAGLY
                     FVYTHLKLQQVVVPAYSQGTVEVFVTVVQDFGQRAVLNLSLDVGPSSTSTITTSESSLI
                     FLHPGERLRLSMSATAGDFLDWQLVSGANTFGLFWVAGGQPLTDSGTLTVIN"
     CDS             4306..5097
                     /codon_start=1
                     /label=TEV_digest_frag1
                     /translation="APVDVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRP
                     YEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERV
                     MNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQY
                     ERAEGRHSTGGMDELYKASHHHHHHSAGSAAGSGEFENLYFQ"
     primer_bind     4307..4333
                     /label=pAF137-2a.FOR
     CDS             4312..4317
                     /codon_start=1
                     /label=SalI
                     /translation="VD"
     CDS             4318..5022
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     4318..4333
                     /label=pAF137-2.FOR
     primer_bind     complement(5004..5022)
                     /label=pAF137-2.REV
     misc_feature    5023..5051
                     /label=Gibson_overlap_3'_site1
     misc_feature    5023..5028
                     /label=NheI
                     /note="NheI restriction digest site"
     CDS             5029..5046
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5047..5079
                     /codon_start=1
                     /product="Chen, X., et al. 2013. Advanced Drug Delivery 
                     Reviews. Fusion protein linkers: Property, design and 
                     functionality Waldo, G. S., et al. 1999. Nat Biotech. Rapid
                     protein-folding assay using green fluorescent protein 
                     Pedelacq, J.-D., et al. 2006. Nat Biotech. Engineering and 
                     characterization of a superfolder green fluorescent 
                     protein"
                     /label=GSAGSAAGSGEF linker
                     /translation="SAGSAAGSGEF"
     primer_bind     5074..5128
                     /label=pAF92.FOR
     misc_feature    5074..5106
                     /label=Gibson_overlap_5'_insert2
     CDS             5080..5100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5098..5658
                     /codon_start=1
                     /label=pAF92_TEV_digest_frag2
                     /translation="GPGRANLAHPHAAEGLSAPQFPAQSPYAQLVMKDALVQNQTLSWH
                     NDPHLSRVFLDPRMNYDADKGELEVKVAGLYFVYTHLKLQQVVVPAYSQGTVEVFVTVV
                     QDFGQRAVLNLSLDVGPSSTSTITTSESSLIFLHPGERLRLSMSATAGDFLDWQLVSGA
                     NTFGLFWVAGGQPLTDSGTLTVIN"
     misc_feature    5101..5106
                     /label=XmaI
                     /note="XmaI restriction digest site. Addition based (and 
                     amino acids) were added to create this site."
     CDS             5107..5658
                     /codon_start=1
                     /gene="LOC100917236"
                     /product="tumor necrosis factor ligand superfamily member 
                     9-like"
                     /label=sha_TNFSF9_ XM_003762879.1
                     /db_xref="GI:395517526"
                     /db_xref="GeneID:100917236"
                     /protein_id="XP_003762927.1"
                     /translation="RANLAHPHAAEGLSAPQFPAQSPYAQLVMKDALVQNQTLSWHNDP
                     HLSRVFLDPRMNYDADKGELEVKVAGLYFVYTHLKLQQVVVPAYSQGTVEVFVTVVQDF
                     GQRAVLNLSLDVGPSSTSTITTSESSLIFLHPGERLRLSMSATAGDFLDWQLVSGANTF
                     GLFWVAGGQPLTDSGTLTVIN"
     misc_feature    5245..5247
                     /label=TNFRSF9_binding
     misc_feature    5281..5283
                     /label=TNFRSF9_binding
     misc_feature    5569..5571
                     /label=TNFRSF9_binding
     primer_bind     complement(5636..5693)
                     /label=pAF92.REV
     misc_feature    5662..5686
                     /label=Gibson_overlap_3'_insert2
     misc_feature    5662..5669
                     /label=PmeI
     misc_feature    5670..5682
                     /label=3'-SfiI
                     /note="3'-SfiI;GGCCTGTCAGGCC;color: #a6acb3"
     polyA_signal    5683..5980
                     /label=bGH-pA
                     /note="bGH-pA;bovine growth hormone polyadenylation 
                     signal;color: #cc0000; direction: LEFT"
     primer_bind     complement(5715..5733)
                     /label=pSB_bGH_seq.REV
     misc_feature    5720..5737
                     /label=BGH_rev_primer
     primer_bind     5720..5737
                     /label=BGH.FOR
     polyA_signal    5726..5950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5991..6032
                     /label=5'-proxITR
                     /note="5'-proxITR;modified region proximal to 5'-ITR - 
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    6038..6262
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6258..6262
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     enhancer        6312..6615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6316..6603
                     /label=CAG_enhancer
     promoter        6321..6813
                     /label=CMV_immearly_promoter
     promoter        6616..6819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    6770..6790
                     /label=CMV_fwd_primer
     primer_bind     6770..6790
                     /label=CMV_fwd_primer
     promoter        6771..6840
                     /label=CMV_promoter
     promoter        6783..6902
                     /label=CMV2_promoter
     misc_feature    6814..6833
                     /label=pCEP_fwd_primer
     misc_feature    6816..6840
                     /label=LNCX_primer
     misc_feature    6957..7053
                     /label=modSV40_late_16s_int
     intron          6957..7053
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    6957..6987
                     /label=SV40_late_19s_int
     primer_bind     7110..7129
                     /label=T7.FOR
     promoter        7110..7128
                     /label=T7_promoter
     CDS             7139..8161
                     /codon_start=1
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    8178..8312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8202..8221)
                     /label=EBV_rev_primer
     primer_bind     complement(8202..8221)
                     /label=EBV.REV
     primer_bind     complement(8256..8275)
                     /label=SV40_pAn1.REV
     primer_bind     complement(8354..8373)
                     /label=SV40_pAn2.REV, pVitro1R
     promoter        8465..8569
                     /gene="bla"
                     /label=bla AmpR promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
     gene            8570..9430
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             8570..9427
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: 1: 5975 .. 6766 / #ccffcc 2: 6767 .. 6835 / 
                     #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggatc cctatacagt
      781 tgaagtcgga agtttacata caccttagcc aaatacattt aaactcactt tttcacaatt
      841 cctgacattt aatcctagta aaaattccct gtcttaggtc agttaggatc accactttat
      901 tttaagaatg tgaaatatca gaataatagt agagagaatg attcatttca gcttttattt
      961 ctttcatcac attcccagtg ggtcagaagt ttacatacac tcgactcctc tgcagaatgc
     1021 ggcgatgttt cggtaagggg tcccgagtag ctagttcatg gcagccagca tatggcatat
     1081 gttgccaaac tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat
     1141 gtccttccga gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc
     1201 ctttattagc cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca
     1261 gagggaaaaa gatctcagtg gtatttgtga gccagggcat tggccacacc agccaccacc
     1321 ttctgatagg cagcctgcac ctgaggagtg aattcacgac aggccttcga actattcctt
     1381 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1441 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1501 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1561 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1621 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     1681 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     1741 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     1801 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     1861 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     1921 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     1981 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2041 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2101 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2161 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2221 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2281 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2341 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatggt
     2401 ggcctcaggt gcagaggttt ctacagggaa agagagaaag gctacagtta gtttgctgtg
     2461 atgatgctaa gtgccgaggt cagatgtcta tggattgtac acggaatccc aacgcaggaa
     2521 ccaaaaaaca ctcacccaaa aaaatggcgc taaggccgag agaaaggcga agttccgtct
     2581 acggctatat accttgaaac aggggcggca gaagtgacgc aacgcggttg cgcggacggc
     2641 ttcctgtgtt ttcattggtc gaagcgttct gcgcgtgcgc agagattccc cagggaaggc
     2701 gggccctctt ccgaatgtcc aatccggaca ttcttccggt tggacctccg cggtcagcgc
     2761 tgtgcccggc gagagatcac gtggggcgcg gaggcggtgc tgctggggca cggccgtcca
     2821 gcctcggcgg ccatattttt gaggggctgt tcatctcgtt cacacgctct gtccgccatg
     2881 tttgtgagtg gaagcgccat taccccttca agcgactgaa ggctgcaggg cctctggtgg
     2941 cccgcatggg gagaccagac ccgccaggcc cgcctttccg cactcagtcc gggcttactt
     3001 tattttgtga gacagggtct cgcctagagg ctccggtgcc cgtcagtggg cagagcgcac
     3061 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3121 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3181 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3241 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3301 gggttatggc ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc
     3361 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     3421 ccccttcgcc tcgtgcttga gttgaggctt ggcctgggcg ctggggccgc cgcgtgcgaa
     3481 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     3541 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     3601 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     3661 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     3721 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     3781 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     3841 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     3901 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     3961 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     4021 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccatactgag tgggtggaga
     4081 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     4141 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccattta
     4201 aggtgtcgtg aaaactaccc caagctggcc tctgcggccg ccaccatgta cagcatgcag
     4261 ctcgcatcct gtcttgcact gacgctcgca ctccttgtca gcagtgcacc cgtcgacgtg
     4321 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     4381 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4441 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     4501 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     4561 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4621 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     4681 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     4741 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     4801 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     4861 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     4921 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     4981 gagggccgcc actccaccgg cggcatggac gagctgtaca aggctagcca tcatcaccat
     5041 caccattccg ctggctccgc tgctggttct ggcgaattcg agaaccttta cttccaggga
     5101 cccgggaggg caaatcttgc ccaccctcac gccgccgaag ggctctctgc cccccagttt
     5161 cctgctcaga gcccctatgc ccagctggtg atgaaagatg ccttggtgca gaaccagaca
     5221 ctgagctggc acaatgatcc tcatctttct cgtgtgttcc tggacccacg aatgaattac
     5281 gatgctgata aaggagagct ggaggtcaaa gtcgccgggc tttacttcgt ctacactcac
     5341 ctgaagctgc agcaggttgt ggttcctgca tacagtcaag gtacagtcga ggtctttgtg
     5401 actgtggtgc aggactttgg acagcgagct gtcttgaatc tctccctgga tgtgggacct
     5461 tccagcactt ccaccatcac cacctcggag tcctccctca tcttcctgca tcctggggag
     5521 cggctcaggc tcagcatgag cgccacagca ggggacttcc tggactggca gctggtgtca
     5581 ggggccaata cctttggcct tttctgggtg gccgggggac agcccttaac cgattctggg
     5641 acattaactg tgatcaatta ggtttaaacg gcctgtcagg ccaagcttgg ggatcaattc
     5701 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5761 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5821 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5881 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5941 ggctctatgg cttctgaggc ggaaagaacc agctgggcct actagatagc ggacccctta
     6001 ccgaaacatc gccgcattct gcagaggagt cgaacttagt gtatgtaaac ttctgaccca
     6061 ctggaattgt gatacagtga attataagtg aaataatctg tctgtaaaca attgttggaa
     6121 aaatgacttg tgtcatgcac aaagtagatg tcctaactga cttgccaaaa ctattgtttg
     6181 ttaacaagaa atttgtggag tagttgaaaa acgagtttta atgactccaa cttaagtgta
     6241 tgtaaacttc cgacttcaac tgtataggga tccaatttta acaaaatatt cgagcttgca
     6301 tgcctgcagg tcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     6361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     6421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     6481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     6541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     6601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     6661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     6721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     6781 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     6841 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     6901 ctccggactc tagaggatcc ggtactcgag gaactgaaaa accagaaagt taactggtaa
     6961 gtttagtctt tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa
     7021 ctgctcctca gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc
     7081 taaaagctgc ggaattgtac ccgcggaatt aatacgactc actataggga ctagtaccat
     7141 gggaaaatca aaagaaatca gccaagacct cagaaaaaga attgtagacc tccacaagtc
     7201 tggttcatcc ttgggagcaa tttccaaacg cctggcggta ccacgttcat ctgtacaaac
     7261 aatagtacgc aagtataaac accatgggac cacgcagccg tcataccgct caggaaggag
     7321 acgcgttctg tctcctagag atgaacgtac tttggtgcga aaagtgcaaa tcaatcccag
     7381 aacaacagca aaggaccttg tgaagatgct ggaggaaaca ggtacaaaag tatctatatc
     7441 cacagtaaaa cgagtcctat atcgacataa cctgaaaggc cactcagcaa ggaagaagcc
     7501 actgctccaa aaccgacata agaaagccag actacggttt gcaactgcac atggggacaa
     7561 agatcgtact ttttggagaa atgtcctctg gtctgatgaa acaaaaatag aactgtttgg
     7621 ccataatgac catcgttatg tttggaggaa gaagggggag gcttgcaagc cgaagaacac
     7681 catcccaacc gtgaagcacg ggggtggcag catcatgttg tgggggtgct ttgctgcagg
     7741 agggactggt gcacttcaca aaatagatgg catcatggac gcggtgcagt atgtggatat
     7801 attgaagcaa catctcaaga catcagtcag gaagttaaag cttggtcgca aatgggtctt
     7861 ccaacacgac aatgacccca agcatacttc caaagttgtg gcaaaatggc ttaaggacaa
     7921 caaagtcaag gtattggagt ggccatcaca aagccctgac ctcaatccta tagaaaattt
     7981 gtgggcagaa ctgaaaaagc gtgtgcgagc aaggaggcct acaaacctga ctcagttaca
     8041 ccagctctgt caggaggaat gggccaaaat tcacccaaat tattgtggga agcttgtgga
     8101 aggctacccg aaacgtttga cccaagttaa acaatttaaa ggcaatgcta ccaaatacta
     8161 ggggccctaa ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     8221 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     8281 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8341 ttcaggttca gggggaggtg tgggaggttt tttaacgctt acaatttcct gatgcggtat
     8401 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     8461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
