LOCUS       Exported                2302 bp ds-DNA     circular SYN 05-JUN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   AVA
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene 5.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     24..46
                     /label=AVA037
     promoter        82..330
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     189..208
                     /label=KZ396
     primer_bind     253..272
                     /label=LKO.1 5'
     primer_bind     complement(312..353)
                     /label=AVA091
     primer_bind     326..374
                     /label=AVA092
     misc_feature    331..350
                     /label=spacer
     misc_RNA        351..426
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    427..440
                     /label=RT template
     misc_feature    436
                     /label=G to T
     misc_feature    441..455
                     /label=annealing
     primer_bind     complement(478..501)
                     /label=AVA038
     rep_origin      complement(553..1141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(716..735)
                     /label=KZ397
     primer_bind     1216..1235
                     /label=ACK562
     CDS             complement(1312..2172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1718..1737
                     /label=ACK563
     promoter        complement(2173..2277)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(2214..2242)
                     /label=amp prom
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gtcatcttag tcattacctg gttttagagc
      361 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      421 cggtgcaacg aacacatcag gtaatgacta agatgttttt ttgggccgct cgaggtacct
      481 ctctacatat gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      961 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1441 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1501 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1621 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1921 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2281 atttccccga aaagtgccac ct
//
