LOCUS Exported 14030 bp ds-DNA circular SYN 28-SEP-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14030) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4 https://www.snapgene.com COMMENT AsiS1+XhoI replace copGFP for TK FEATURES Location/Qualifiers source 1..14030 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 49..289 /label=U6 Promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_RNA 318..393 /label=gRNA scaffold /note="guide RNA scaffold for the CRISPR/Cas9 system" misc_feature 441..558 /label=cPPT misc_feature 602..852 /label=EFS CDS 867..890 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 897..917 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 942..5042 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5043..5090 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5106..5162 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 5163..5759 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" CDS 5787..5840 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" misc_feature 5860..6602 /label=copGFP polyA_signal 6653..6787 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature 7021..9303 /label=EBV misc_feature 9304..11913 /label=EBV_genomic misc_feature 12024..12036 /label=pcr fwd promoter 12050..12154 /gene="bla" /label=AmpR promoter CDS 12155..13015 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 13186..13774 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cattatggta cctttcagac ccacctccca accccgaggg gacccagaga gggcctattt 61 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt 121 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt 181 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta 241 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgag 301 aagagcgatg ctcttcggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt 361 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat cttgagacaa 421 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 481 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 541 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg 601 ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 661 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg 721 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 781 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt 841 gtcgtgacgc gggatccgcc accatggatt acaaagacga tgacgataag atggccccaa 901 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg 961 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc 1021 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga 1081 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg 1141 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca 1201 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg 1261 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct 1321 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca 1381 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact 1441 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc 1501 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg 1561 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc 1621 agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc 1681 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca 1741 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg 1801 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag 1861 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc 1921 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca 1981 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca 2041 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg 2101 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg 2161 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag 2221 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca 2281 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa 2341 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg 2401 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc 2461 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga 2521 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca 2581 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact 2641 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca 2701 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg 2761 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg 2821 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga 2881 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca 2941 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct 3001 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca 3061 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg 3121 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg 3181 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc 3241 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca 3301 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga 3361 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac 3421 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga 3481 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg 3541 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga 3601 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc 3661 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca 3721 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca 3781 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga 3841 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct 3901 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt 3961 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg 4021 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga 4081 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg 4141 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga 4201 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag 4261 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc 4321 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca 4381 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca 4441 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca 4501 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa 4561 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc 4621 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct 4681 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg 4741 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg 4801 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga 4861 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg 4921 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga 4981 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg 5041 acaagcgtcc tgctgctact aagaaagctg gtcaagctaa gaaaaagaaa gctagcggca 5101 gcggcgccac caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc 5161 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 5221 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 5281 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 5341 acatcggcaa ggtgtgggtc gcggacgacg gcgcggccgt ggcggtctgg accacgccgg 5401 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 5461 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 5521 agcccgcgtg gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg 5581 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 5641 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 5701 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg 5761 cgatcgccga aggatccgcg gccgctgagg gcagaggaag tcttctaaca tgcggtgacg 5821 tggaggagaa tcccggccct tccggaatgg agagcgacga gagcggcctg cccgccatgg 5881 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg 5941 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaaaagcacc aaaggcgccc 6001 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca 6061 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca 6121 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct 6181 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca 6241 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca 6301 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct 6361 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc 6421 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg 6481 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc 6541 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc 6601 gctagagctg aatctaagtc gacttaagaa ccgctcgagg ccggcaaggc cggatccaga 6661 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 6721 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 6781 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 6841 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc 6901 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 6961 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 7021 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatg ccccgccccg 7081 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc 7141 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg 7201 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat 7261 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac 7321 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt 7381 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg 7441 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc 7501 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta 7561 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag 7621 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta 7681 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa 7741 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt 7801 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa 7861 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg 7921 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat 7981 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac 8041 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg 8101 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta 8161 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt 8221 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga 8281 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc 8341 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc 8401 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct 8461 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt 8521 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg 8581 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata 8641 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta 8701 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta 8761 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta 8821 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta 8881 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat 8941 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat 9001 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat 9061 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat 9121 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat 9181 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta 9241 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc 9301 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc 9361 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg 9421 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg 9481 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta 9541 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt 9601 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc 9661 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac 9721 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat 9781 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac 9841 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc 9901 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt 9961 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac 10021 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc 10081 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca 10141 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc 10201 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc 10261 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg 10321 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg 10381 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc 10441 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc 10501 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc 10561 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc 10621 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc 10681 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc 10741 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc 10801 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc 10861 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc 10921 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc 10981 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc 11041 tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc 11101 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc 11161 ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc 11221 tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc 11281 tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg 11341 ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc 11401 ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc 11461 tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc 11521 gtcagacatg attcacacta aaagagatca atagacatct ttattagacg acgctcagtg 11581 aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat 11641 ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct 11701 catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct 11761 gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg 11821 attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa 11881 cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca 11941 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 12001 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 12061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 12121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 12181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 12241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 12301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 12361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact 12421 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 12481 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 12541 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 12601 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 12661 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg 12721 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 12781 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 12841 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 12901 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 12961 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 13021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 13081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 13141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 13201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 13261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 13321 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 13381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 13441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 13501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 13561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 13621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 13681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 13741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 13801 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga 13861 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg 13921 gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg tgtcagttag 13981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg //