LOCUS       Exported               14030 bp ds-DNA     circular SYN 28-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
COMMENT     AsiS1+XhoI replace copGFP for TK
FEATURES             Location/Qualifiers
     source          1..14030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        49..289
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        318..393
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     misc_feature    441..558
                     /label=cPPT
     misc_feature    602..852
                     /label=EFS
     CDS             867..890
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             897..917
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             942..5042
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5043..5090
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5106..5162
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5163..5759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             5787..5840
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5860..6602
                     /label=copGFP
     polyA_signal    6653..6787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7021..9303
                     /label=EBV
     misc_feature    9304..11913
                     /label=EBV_genomic
     misc_feature    12024..12036
                     /label=pcr fwd
     promoter        12050..12154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12155..13015
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13186..13774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cattatggta cctttcagac ccacctccca accccgaggg gacccagaga gggcctattt
       61 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
      121 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      181 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      241 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgag
      301 aagagcgatg ctcttcggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      361 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat cttgagacaa
      421 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      481 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      541 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
      601 ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      661 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
      721 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      781 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
      841 gtcgtgacgc gggatccgcc accatggatt acaaagacga tgacgataag atggccccaa
      901 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg
      961 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     1021 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     1081 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     1141 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     1201 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     1261 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     1321 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     1381 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     1441 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     1501 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     1561 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     1621 agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc
     1681 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     1741 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1801 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1861 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1921 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1981 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     2041 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     2101 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     2161 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     2221 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     2281 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     2341 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     2401 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     2461 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     2521 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     2581 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     2641 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     2701 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     2761 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2821 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2881 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2941 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     3001 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     3061 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     3121 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     3181 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     3241 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     3301 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     3361 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     3421 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     3481 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     3541 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     3601 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     3661 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     3721 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3781 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3841 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3901 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3961 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     4021 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     4081 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     4141 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     4201 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     4261 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     4321 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     4381 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     4441 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     4501 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     4561 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     4621 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     4681 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     4741 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4801 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4861 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4921 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4981 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     5041 acaagcgtcc tgctgctact aagaaagctg gtcaagctaa gaaaaagaaa gctagcggca
     5101 gcggcgccac caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc
     5161 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5221 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5281 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5341 acatcggcaa ggtgtgggtc gcggacgacg gcgcggccgt ggcggtctgg accacgccgg
     5401 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5461 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5521 agcccgcgtg gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg
     5581 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5641 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5701 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg
     5761 cgatcgccga aggatccgcg gccgctgagg gcagaggaag tcttctaaca tgcggtgacg
     5821 tggaggagaa tcccggccct tccggaatgg agagcgacga gagcggcctg cccgccatgg
     5881 agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg gtgggcggcg
     5941 gagagggcac ccccaagcag ggccgcatga ccaacaagat gaaaagcacc aaaggcgccc
     6001 tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac cacttcggca
     6061 cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc ggctacacca
     6121 acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc agctaccgct
     6181 acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc cccgaggaca
     6241 gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac ctgcacccca
     6301 tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc gacggcggct
     6361 actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac cccagcatcc
     6421 tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg
     6481 agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc gccagatccc
     6541 gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc accggatctc
     6601 gctagagctg aatctaagtc gacttaagaa ccgctcgagg ccggcaaggc cggatccaga
     6661 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     6721 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     6781 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     6841 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
     6901 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6961 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7021 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatg ccccgccccg
     7081 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     7141 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     7201 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     7261 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     7321 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     7381 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     7441 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     7501 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     7561 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     7621 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     7681 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     7741 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     7801 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     7861 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     7921 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     7981 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     8041 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     8101 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     8161 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     8221 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     8281 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     8341 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     8401 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     8461 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     8521 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     8581 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     8641 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     8701 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     8761 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8821 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     8881 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     8941 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     9001 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     9061 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9121 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     9181 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     9241 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     9301 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     9361 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     9421 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     9481 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     9541 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     9601 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     9661 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     9721 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     9781 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     9841 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     9901 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     9961 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
    10021 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
    10081 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
    10141 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
    10201 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
    10261 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
    10321 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
    10381 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
    10441 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
    10501 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
    10561 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
    10621 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
    10681 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
    10741 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
    10801 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
    10861 ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
    10921 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
    10981 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
    11041 tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
    11101 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
    11161 ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
    11221 tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc
    11281 tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg
    11341 ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc
    11401 ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc
    11461 tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc
    11521 gtcagacatg attcacacta aaagagatca atagacatct ttattagacg acgctcagtg
    11581 aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat
    11641 ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct
    11701 catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct
    11761 gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg
    11821 attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa
    11881 cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca
    11941 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    12001 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    12061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    12121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    12181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    12241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    12301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    12361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    12421 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    12481 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    12541 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    12601 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    12661 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
    12721 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    12781 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    12841 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    12901 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    12961 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    13021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    13081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    13141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    13201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    13261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    13321 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    13381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    13441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    13501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    13561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    13621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    13681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    13741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    13801 gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
    13861 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
    13921 gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg tgtcagttag
    13981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
//
