LOCUS       Exported               14060 bp ds-DNA     circular SYN 28-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   ymw
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    8..328
                     /label=mU6
     misc_RNA        348..423
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     misc_feature    471..588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    632..882
                     /label=EFS
     CDS             897..920
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             927..947
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    972..5072
                     /label=eSpCas9(1.1)
     CDS             5073..5120
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5136..5192
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5193..5789
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             5817..5870
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5890..6632
                     /label=copGFP
     polyA_signal    6683..6817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7051..11943
                     /label=EBNA genomic
     promoter        12080..12184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12185..13045
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13216..13804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cattatgtct agagatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga
       61 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag
      121 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct
      181 acattttaca tgataggctt ggatttctat aagagataca aatactaaat tattatttta
      241 aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat
      301 aaatatgcat gcgagaaaag ccttgtttag aagagcgatg ctcttcggtt ttagagctag
      361 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      421 tgcttttttg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      481 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      541 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      601 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc
      661 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      721 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      781 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      841 tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gggatccgcc accatggatt
      901 acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag
      961 tcccagcagc cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct
     1021 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     1081 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     1141 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     1201 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     1261 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     1321 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     1381 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     1441 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     1501 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     1561 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     1621 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     1681 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     1741 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     1801 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     1861 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     1921 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     1981 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     2041 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     2101 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     2161 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     2221 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     2281 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     2341 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     2401 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     2461 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     2521 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     2581 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     2641 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     2701 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     2761 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     2821 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     2881 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     2941 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     3001 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     3061 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     3121 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     3181 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     3241 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     3301 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     3361 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     3421 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     3481 tggaccatat cgtgcctcag agctttctgg cggacgactc catcgacaac aaggtgctga
     3541 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     3601 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     3661 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     3721 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     3781 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     3841 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     3901 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     3961 tcaaaaagta ccctgcgctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     4021 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     4081 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     4141 ggaaggcgcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
     4201 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
     4261 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
     4321 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     4381 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     4441 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     4501 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     4561 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     4621 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     4681 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     4741 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
     4801 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
     4861 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     4921 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     4981 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     5041 cacggatcga cctgtctcag ctgggaggcg acaagcgtcc tgctgctact aagaaagctg
     5101 gtcaagctaa gaaaaagaaa gctagcggca gcggcgccac caacttcagc ctgctgaagc
     5161 aggccggcga cgtggaggag aaccccggcc ccatgaccga gtacaagccc acggtgcgcc
     5221 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5281 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5341 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5401 gcgcggccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5461 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5521 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag
     5581 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5641 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5701 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5761 cctggtgcat gacccgcaag cccggtgccg cgatcgccga aggatccgcg gccgctgagg
     5821 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct tccggaatgg
     5881 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga
     5941 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga
     6001 ccaacaagat gaaaagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg
     6061 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc
     6121 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg
     6181 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca
     6241 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca
     6301 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg
     6361 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc
     6421 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc
     6481 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct
     6541 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg
     6601 gcaccgccgg acccggctcc accggatctc gctagagctg aatctaagtc gacttaagaa
     6661 ccgctcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     6721 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6781 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6841 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6901 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6961 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7021 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     7081 tcgactctag aggatcgatg ccccgccccg gacgaactaa acctgactac gacatctctg
     7141 ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact
     7201 gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta
     7261 gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga
     7321 gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg
     7381 aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga
     7441 ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag
     7501 ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt
     7561 cccgggtagt agtatatact atccagacta accctaattc aatagcatat gttacccaac
     7621 gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc
     7681 ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg agggtagtga
     7741 accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga
     7801 atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga
     7861 acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa
     7921 atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac
     7981 cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa
     8041 atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct
     8101 atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc
     8161 agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt
     8221 ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg
     8281 ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg
     8341 gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg
     8401 gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca
     8461 aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg
     8521 gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca
     8581 gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg
     8641 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     8701 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     8761 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     8821 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     8881 gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta
     8941 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     9001 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9061 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     9121 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     9181 atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat
     9241 atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg
     9301 ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt
     9361 aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg
     9421 ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc
     9481 caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca
     9541 acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt
     9601 aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc
     9661 tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac
     9721 cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct
     9781 gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac
     9841 ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag
     9901 cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt
     9961 aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt
    10021 actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac
    10081 gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga
    10141 acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc
    10201 ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat
    10261 cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata
    10321 atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg
    10381 agacccggat gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg
    10441 acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg
    10501 gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact
    10561 gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc
    10621 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
    10681 tcctgcccct cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc
    10741 tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
    10801 tgcccctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
    10861 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgctcc
    10921 tgcccctcct gcccctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc
    10981 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
    11041 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
    11101 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
    11161 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc
    11221 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc
    11281 tgctcctgct cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg
    11341 gggtctccgg acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg
    11401 tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct
    11461 tctttgaggt ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag
    11521 gccatttcca ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca
    11581 atagacatct ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc
    11641 caacagcccc cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa
    11701 attccccatc ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact
    11761 cccgctgaac atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt
    11821 cccccttttt gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa
    11881 ggtcaccaga cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg
    11941 atcagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
    12001 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
    12061 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    12121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    12181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    12241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    12301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    12361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    12421 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    12481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    12541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    12601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    12661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    12721 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    12781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    12841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    12901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    12961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    13021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    13081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    13141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13321 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    13381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    13501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    13561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    13621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    13681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    13741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    13801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg
    13861 tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat
    13921 gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgaactaga
    13981 tgatccggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    14041 gcagaagtat gcaaagcatg
//
