LOCUS       Exported               13578 bp ds-DNA     circular SYN 17-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 6, 2019 from SnapGene Viewer 5.0.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13578
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        49..289
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        318..393
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    441..558
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        627..838
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             867..890
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             897..917
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             942..5042
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5043..5090
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5106..5162
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5163..6170
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     6182..6201
                     /label=Ratio-F
     polyA_signal    6201..6335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7061..8850
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        11598..11702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11703..12563
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12734..13322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cattatggta cctttcagac ccacctccca accccgaggg gacccagaga gggcctattt
       61 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt
      121 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
      181 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
      241 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgag
      301 aagagcgatg ctcttcggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      361 tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat cttgagacaa
      421 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      481 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      541 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
      601 ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      661 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
      721 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      781 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
      841 gtcgtgacgc gggatccgcc accatggatt acaaagacga tgacgataag atggccccaa
      901 agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg
      961 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     1021 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     1081 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     1141 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     1201 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     1261 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     1321 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     1381 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     1441 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     1501 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     1561 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     1621 agctgcccgg cgagaagaag aatggcctgt tcggcaacct gattgccctg agcctgggcc
     1681 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     1741 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     1801 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     1861 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     1921 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     1981 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     2041 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     2101 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     2161 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     2221 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     2281 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     2341 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     2401 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     2461 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     2521 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     2581 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     2641 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     2701 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     2761 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     2821 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     2881 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     2941 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     3001 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     3061 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     3121 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     3181 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     3241 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     3301 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     3361 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     3421 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     3481 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     3541 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     3601 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     3661 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     3721 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     3781 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     3841 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     3901 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     3961 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     4021 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     4081 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     4141 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     4201 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     4261 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     4321 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     4381 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     4441 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     4501 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     4561 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     4621 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     4681 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     4741 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     4801 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     4861 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     4921 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     4981 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     5041 acaagcgtcc tgctgctact aagaaagctg gtcaagctaa gaaaaagaaa gctagcggca
     5101 gcggcgccac caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc
     5161 ccatgtctag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     5221 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
     5281 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     5341 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     5401 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     5461 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     5521 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
     5581 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     5641 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     5701 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     5761 aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt
     5821 ctaccatcga gggcctgctc gatctcccgg acgacgacgc ccccgaagag gcggggctgg
     5881 cggctccgcg cctgtccttt ctccccgcgg gacacacgcg cagactgtcg acggcccccc
     5941 cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg gcgatggcgc
     6001 atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat tccccgggtc
     6061 cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc gacttcgagt
     6121 ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtag cttaagaacc
     6181 gctcgaggcc ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa
     6241 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6301 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6361 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     6421 gtggtatggc tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     6481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc
     6601 gactctagag gatcgatgcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     6661 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     6721 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     6781 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     6841 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     6901 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     6961 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     7021 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     7081 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     7141 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     7201 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     7261 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     7321 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     7381 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     7441 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     7501 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     7561 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     7621 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     7681 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     7741 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     7801 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     7861 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     7921 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     7981 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     8041 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     8101 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     8161 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     8221 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8281 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8341 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     8401 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     8461 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     8521 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     8581 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     8641 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     8701 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
     8761 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
     8821 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
     8881 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
     8941 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
     9001 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
     9061 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
     9121 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
     9181 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
     9241 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
     9301 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
     9361 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
     9421 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
     9481 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
     9541 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
     9601 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
     9661 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
     9721 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
     9781 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
     9841 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
     9901 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
     9961 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
    10021 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
    10081 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
    10141 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
    10201 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
    10261 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
    10321 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
    10381 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
    10441 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
    10501 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
    10561 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
    10621 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
    10681 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
    10741 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
    10801 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
    10861 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
    10921 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
    10981 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
    11041 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
    11101 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
    11161 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
    11221 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
    11281 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
    11341 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
    11401 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
    11461 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
    11521 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
    11581 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    11641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    11701 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    11761 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    11821 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11881 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11941 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    12001 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    12061 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    12121 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    12181 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    12241 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    12301 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    12361 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    12421 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    12481 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    12541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    12601 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    12661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12841 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    12901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    13021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    13081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    13141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    13201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    13261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    13321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
    13381 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
    13441 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gaactagatg
    13501 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    13561 agaagtatgc aaagcatg
//
