LOCUS       Exported                7336 bp ds-DNA     circular SYN 12-NOV-2019
DEFINITION  pcDNA3.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-His:DmrC:nls:VPR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 12, 2019 from SnapGene 5.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    206..235
                     /label=pimp my pCDNA3 -r
                     /note="color: #a6acb3"
     promoter        236..852
                     /label=CMV_immearly_promoter
                     /note="color: #ffffff"
     misc_feature    315..602
                     /label=CAG_enhancer
                     /note="color: #a6acb3"
     misc_feature    769..789
                     /label=CMV_fwd_primer
                     /note="color: #a6acb3"
     promoter        863..881
                     /label=T7_promoter
                     /note="color: #ffffff"
     misc_signal     944..949
                     /label=Kozakovo zaporedje
                     /note="color: #a6acb3"
     misc_feature    950..952
                     /label=start kodon
                     /note="color: #a6acb3"
     misc_feature    953..970
                     /label=HIS tag
                     /note="color: #ffff99"
     misc_feature    971..1258
                     /label=FRB
                     /note="color: #00ff00"
     misc_signal     1259..1282
                     /label=NLS
                     /note="color: #3366ff"
     misc_feature    1283..2872
                     /label=VPR
                     /note="color: #008000"
     misc_feature    complement(2889..2906)
                     /label=Sp6_primer
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(2890..2907)
                     /label=Sp6_promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(2926..2943)
                     /label=BGH_rev_primer
                     /note="color: #a6acb3; direction: LEFT"
     terminator      2929..3156
                     /label=bGH_PA_terminator
                     /note="color: #ffffff"
     primer_bind     3157..3186
                     /label=pimp my pCDNA3- F
                     /note="color: #a020f0; direction: RIGHT"
     rep_origin      3219..3525
                     /label=f1_origin
                     /note="color: #ffff00"
     misc_feature    complement(3639..3659)
                     /label=pBABE_3_primer
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(3645..3861)
                     /label=SV40_enhancer
                     /note="color: #a6acb3; direction: LEFT"
     promoter        3657..3926
                     /label=SV40_promoter
                     /note="color: #ffffff"
     rep_origin      3825..3902
                     /label=SV40_origin
                     /note="color: #ffff00"
     misc_feature    3887..3906
                     /label=SV40pro_F_primer
                     /note="color: #a6acb3"
     CDS             4041..4835
                     /gene="Neomycin"
                     /label=Neomycin
                     /note="ORF frame 3"
                     /note="color: #993366; direction: RIGHT"
     gene            4044..4832
                     /gene="Neomycin"
                     /label=Neomycin
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5012..5131
                     /label=SV40_PA_terminator
                     /note="color: #ffffff"
     misc_feature    5100..5119
                     /label=EBV_rev_primer
                     /note="color: #a6acb3"
     misc_feature    complement(5175..5193)
                     /label=M13_reverse_primer
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(5192..5214)
                     /label=M13_pUC_rev_primer
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(5228..5257)
                     /label=lac_promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      complement(5566..6185)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="color: #ffff00"
     CDS             complement(6340..7200)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="ORF frame 1"
                     /note="color: #993366; direction: LEFT"
     gene            complement(6340..7200)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="color: #a6acb3; direction: LEFT"
     promoter        complement(7242..7270)
                     /label=AmpR_promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca
      961 ccaccaccac gcttctagaa tcctctggca tgagatgtgg catgaaggcc tggaagaggc
     1021 atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt
     1081 gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta
     1141 tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt
     1201 caaggacctc ctccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaagga
     1261 tccaaaaaag aagagaaagg tagaggccag cggttcggga cgggctgacg cattggacga
     1321 ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg acatgcttgg
     1381 ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc ttgatgattt
     1441 cgacctggac atgctgatta acactagtgg cggctctggc ggcggctccg gaggctctag
     1501 ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta
     1561 cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc
     1621 tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc
     1681 ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat
     1741 ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt
     1801 gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc
     1861 accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc
     1921 taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga
     1981 cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct
     2041 ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc ctgtggcccc
     2101 tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg
     2161 cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg
     2221 actgctgtct ggcgacgagg acttcagctc tatcgccgat atggatttct cagccttgct
     2281 gggctctggc agcggcagcc gggattccag ggaagggatg tttttgccga agcctgaggc
     2341 cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg
     2401 gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca gcctcgcacc
     2461 aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac cagtccctca
     2521 gccactggac ccagcgcccg cagtgactcc cgaggccagt cacctgttgg aggaccccga
     2581 tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg tgattcccca
     2641 gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc caaggggcca
     2701 tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc tggactcacc
     2761 cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt gcctcttgca
     2821 tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttt gatctagagg
     2881 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     2941 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3001 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3061 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3121 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3181 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3241 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3301 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     3361 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3421 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3481 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3541 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     3601 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     3661 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     3721 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3781 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3841 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3901 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3961 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     4021 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4081 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4141 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4201 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4261 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4321 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4381 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4441 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4501 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4561 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4621 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4681 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4741 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4801 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4861 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4921 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4981 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5101 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5161 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5341 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5761 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5821 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5881 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5941 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6001 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6061 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6241 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6301 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6361 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6421 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6481 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6541 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6601 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6661 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6721 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6781 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6841 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6901 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6961 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7021 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7081 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7141 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7201 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7261 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7321 agtgccacct gacgtc
//
