LOCUS       Exported                8898 bp ds-DNA     circular SYN 05-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 5, 2019 from SnapGene Viewer 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     misc_feature    1162..4518
                     /label=UPF1
     misc_feature    3067..3072
                     /label=DE647AA Helicase/ATPase Mutant
     promoter        complement(4565..4583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    4600..4721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4907..5362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5481..5838
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5689..5824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5889..6683
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6747..6795
                     /note="synthetic polyadenylation signal"
     promoter        7101..7205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7206..8066
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8237..8825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac gcttagcgtg gaggcgtacg ggcccagctc gcagactctc
     1201 actttcctgg acacggagga ggccgagctg cttggcgccg acacacaggg ctccgagttc
     1261 gagttcaccg actttactct tcctagccag acgcagacgc cccccggcgg ccccggcggc
     1321 ccgggcggtg gcggcgcggg aggcccgggc ggcgcgggcg cgggcgctgc ggcgggacag
     1381 ctcgacgcgc aggttgggcc cgaaggcatc ctgcagaacg gggctgtgga cgacagtgta
     1441 gccaagacca gccagttgtt ggctgagttg aacttcgagg aagatgaaga agacacctat
     1501 tacacgaagg acctccccat acacgcctgc agttactgtg gaatacacga tcctgcctgc
     1561 gtggtttact gtaataccag caagaagtgg ttctgcaacg gacgtggaaa tacttctggc
     1621 agccacattg taaatcacct tgtgagggca aaatgcaaag aggtgaccct gcacaaggac
     1681 gggcccctgg gggagacagt cctggagtgc tacaactgcg gctgtcgcaa cgtcttcctc
     1741 ctcggcttca tcccggccaa agctgactca gtggtggtgc tgctgtgcag gcagccctgt
     1801 gccagccaga gcagcctcaa ggacatcaac tgggacagct cgcagtggca gccgctgatc
     1861 caggaccgct gcttcctgtc ctggctggtc aagatcccct ccgagcagga gcagctgcgg
     1921 gcacgccaga tcacggcaca gcagatcaac aagctggagg agctgtggaa ggaaaaccct
     1981 tctgccacgc tggaggacct ggagaagccg ggggtggacg aggagccgca gcatgtcctc
     2041 ctgcggtacg aggacgccta ccagtaccag aacatattcg ggcccctggt caagctggag
     2101 gccgactacg acaagaagct gaaggagtcc cagactcaag ataacatcac tgtcaggtgg
     2161 gacctgggcc ttaacaagaa gagaatcgcc tacttcactt tgcccaagac tgactctgac
     2221 atgcggctca tgcaggggga tgagatatgc ctgcggtaca aaggggacct tgcgcccctg
     2281 tggaaaggga tcggccacgt catcaaggtc cctgataatt atggcgatga gatcgccatt
     2341 gagctgcgga gcagcgtggg tgcacctgtg gaggtgactc acaacttcca ggtggatttt
     2401 gtgtggaagt cgacctcctt tgacaggatg cagagcgcat tgaaaacgtt tgccgtggat
     2461 gagacctcgg tgtctggcta catctaccac aagctgttgg gccacgaggt ggaggacgta
     2521 atcatcaagt gccagctgcc caagcgcttc acggcgcagg gcctccccga cctcaaccac
     2581 tcccaggttt atgccgtgaa gactgtgctg caaagaccac tgagcctgat ccagggcccg
     2641 ccaggcacgg ggaagacggt gacgtcggcc accatcgtct accacctggc ccggcaaggc
     2701 aacgggccgg tgctggtgtg tgctccgagc aacatcgccg tggaccagct aacggagaag
     2761 atccaccaga cggggctaaa ggtcgtgcgc ctctgcgcca agagccgtga ggccatcgac
     2821 tccccggtgt cttttctggc cctgcacaac cagatcagga acatggacag catgcctgag
     2881 ctgcagaagc tgcagcagct gaaagacgag actggggagc tgtcgtctgc cgacgagaag
     2941 cggtaccggg ccttgaagcg caccgcagag agagagctgc tgatgaacgc agatgtcatc
     3001 tgctgcacat gtgtgggcgc cggtgacccg aggctggcca agatgcagtt ccgctccatt
     3061 ttaatcgacg aaagcaccca ggccaccgag ccggagtgca tggttcccgt ggtcctcggg
     3121 gccaagcagc tgatccttgt aggcgaccac tgccagctgg gcccagtggt gatgtgcaag
     3181 aaggcggcca aggccgggct gtcacagtcg ctcttcgagc gcctggtggt gctgggcatc
     3241 cggcccatcc gcctgcaggt ccagtaccgg atgcaccctg cactcagcgc cttcccatcc
     3301 aacatcttct acgagggctc cctccagaat ggtgtcactg cagcggatcg tgtgaagaag
     3361 ggatttgact tccagtggcc ccaacccgat aaaccgatgt tcttctacgt gacccagggc
     3421 caagaggaga ttgccagctc gggcacctcc tacctgaaca ggaccgaggc tgcgaacgtg
     3481 gagaagatca ccacgaagtt gctgaaggca ggcgccaagc cggaccagat tggcatcatc
     3541 acgccctacg agggccagcg ctcctacctg gtgcagtaca tgcagttcag cggctccctg
     3601 cacaccaagc tctaccagga ggtggagatc gccagtgtgg acgcctttca gggacgcgag
     3661 aaggacttca tcatcctgtc ctgtgtgcgg gccaacgagc accaaggcat tggcttttta
     3721 aatgacccca ggcgtctgaa cgtggccctg accagagcaa ggtatggcgt catcattgtg
     3781 ggcaacccga aggcactatc aaagcagccg ctctggaacc acctgctgaa ctactataag
     3841 gagcagaagg tgctggtgga ggggccgctc aacaacctgc gtgagagcct catgcagttc
     3901 agcaagccac ggaagctggt caacactatc aacccgggag cccgcttcat gaccacagcc
     3961 atgtatgatg cccgggaggc catcatccca ggctccgtct atgatcggag cagccagggc
     4021 cggccttcca gcatgtactt ccagacccat gaccagattg gcatgatcag tgccggccct
     4081 agccacgtgg ctgccatgaa cattcccatc cccttcaacc tggtcatgcc acccatgcca
     4141 ccgcctggct attttggaca agccaacggg cctgctgcag ggcgaggcac cccgaaaggc
     4201 aagactggtc gtgggggacg ccagaagaac cgctttgggc ttcctggacc cagccagact
     4261 aacctcccca acagccaagc cagccaggat gtggcgtcac agcccttctc tcagggcgcc
     4321 ctgacgcagg gctacatctc catgagccag ccttcccaga tgagccagcc cggcctctcc
     4381 cagccggagc tgtcccagga cagttacctt ggtgacgagt ttaaatcaca aatcgacgtg
     4441 gcgctctcac aggactccac gtaccaggga gagcgggctt accagcatgg cggggtgacg
     4501 gggctgtccc agtattaaaa ggtggcggcg gaagagctaa agagtcgacc cgggcggccg
     4561 cttcccttta gtgagggtta atgcttcgag cagacatgat aagatacatt gatgagtttg
     4621 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4681 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4741 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     4801 acaaatgtgg taaaatccga taaggatcga tccgggctgg cgtaatagcg aagaggcccg
     4861 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     4921 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4981 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5041 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagagc tttacggcac
     5101 ctcgaccgca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     5161 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5221 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5281 atttcggcct attggttaaa aaatgagctg atttaacaaa tatttaacgc gaattttaac
     5341 aaaatattaa cgtttacaat ttcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     5401 tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     5461 ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     5521 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5581 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5641 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5761 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5821 ggcctaggct tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta
     5881 gagccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5941 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6001 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6061 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6121 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6181 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6241 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6301 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6361 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6421 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6481 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6541 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6601 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6661 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6721 gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg
     6781 ttggtttttt gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa
     6841 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6901 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6961 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     7021 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7081 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7141 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7201 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7261 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7321 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7381 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7441 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7501 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7561 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7621 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7681 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7741 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7801 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7861 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7921 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7981 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8041 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8101 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8161 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8221 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8281 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8341 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     8401 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8461 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8521 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8581 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8641 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8701 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8761 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8821 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8881 acatggctcg acagatct
//
