LOCUS       Exported                7774 bp ds-DNA     circular SYN 28-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 28, 2018 from SnapGene Viewer 4.2.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2134..2150
                     /label=M13R
                     /note="M13R"
     misc_feature    2136..2154
                     /label=M13 reverse priming site
                     /note="M13 reverse priming site"
     misc_feature    2177..2180
                     /label=Pme1 site
                     /note="Pme1 site"
     misc_feature    2185..2392
                     /label=5' homology arm
                     /note="5' homology arm"
     enhancer        2406..2783
                     /label=hCMV enhancer
                     /note="hCMV enhancer"
     misc_feature    complement(2729..2750)
                     /label=HN853
                     /note="HN853"
     promoter        2789..4130
                     /label=AG hybrid promoter
                     /note="AG hybrid promoter"
     misc_feature    2789..3066
                     /label=Chicken b-actin promoter
                     /note="Chicken b-actin promoter"
     misc_feature    2976..2980
                     /label=CCATT box
                     /note="CCATT box"
     misc_feature    3038..3045
                     /label=TATA box
                     /note="TATA box"
     misc_feature    3050..3066
                     /label=TSS
                     /note="TSS"
     misc_feature    3067..3157
                     /label=Chicken b-actin exon 1
                     /note="Chicken b-actin exon 1"
     misc_feature    3158..4082
                     /label=Chicken b-actin rabbit globin hybrid
                     /note="Chicken b-actin rabbit globin hybrid intron"
     intron          3158..4033
                     /label=Chicken b-actin intron
                     /note="Chicken b-actin intron"
     misc_feature    4034..4130
                     /label=Rabbit b-globin splice acceptor
                     /note="Rabbit b-globin splice acceptor"
     misc_feature    4047..4067
                     /label=HN737
                     /note="HN737"
     misc_feature    4091..4110
                     /label=HN382
                     /note="HN382"
     gene            complement(4142..4175)
                     /label=loxP
                     /note="loxP"
     misc_feature    4182..4185
                     /label=Untitled
     misc_feature    4202..4429
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    4435..4662
                     /label=SV40 pA
                     /note="SV40 pA"
     misc_feature    4668..4895
                     /label=SV40 pA
                     /note="SV40 pA"
     gene            complement(4918..4951)
                     /label=loxP
                     /note="loxP"
     CDS             4961..5338
                     /label=H2B
                     /note="H2B"
     misc_feature    5142..5154
                     /label=BspH1 blocked by dam methylation
                     /note="BspH1 blocked by dam methylation"
     misc_feature    5150..5152
                     /label=mutated from AAT
                     /note="mutated from AAT"
     misc_feature    5357..6073
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    5357..5387
                     /label=Untitled
     misc_feature    6082..6667
                     /label=WPRE
     misc_feature    6688..6989
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    7006..7119
                     /label=3' homology arm
                     /note="3' homology arm"
     misc_feature    complement(7132..7153)
                     /label=T7 priming site
                     /note="T7 priming site"
     misc_feature    complement(7159..7175)
                     /label=M13 F2 priming site
                     /note="M13 F2 priming site"
     misc_feature    complement(7159..7175)
                     /label=M13F
                     /note="M13F"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaagttt aaacaagggc aggatgtgtc aaactgccaa tagagaacta
     2221 cttactcttc aggctgaagc tgatggaaca ggtaacaaag gcaaacacta atcatgatca
     2281 gcaagatgaa gcagaaaggg aacaagggga tattaaatgt gtatagacac gctagagaga
     2341 tggctcagca gttaagagaa ctagctggtc tttcagaggt cctgagatca ataccggtca
     2401 tggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2461 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2521 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2581 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2641 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2701 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2761 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2821 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     2881 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     2941 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     3001 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     3061 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     3121 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     3181 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     3241 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     3301 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     3361 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     3421 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     3481 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     3541 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     3601 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     3661 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     3721 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     3781 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     3841 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     3901 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     3961 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     4021 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     4081 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgctag
     4141 cataacttcg tatagcatac attatacgaa gttatagcca ccgcggtggc ggccgctcta
     4201 gaatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4261 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ctagaatcat
     4441 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4501 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4621 gcattctagt tgtggtttgt ccaaactcat caatgtatct taactagaat cataatcagc
     4681 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     4741 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     4801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4861 agttgtggtt tgtccaaact catcaatgta tcttaactag tggatctcat gcctaggata
     4921 acttcgtata gcatacatta tacgaagtta taggcgcgcc atgcctgaac cctctaagtc
     4981 tgctccagcc cctaaaaagg gttctaagaa ggctatcact aaggcgcaga agaaggatgg
     5041 taagaagcgt aagcgcagcc gcaaggagag ctattctatc tatgtgtaca aggttctgaa
     5101 gcaggtccac cccgacaccg gcatctcatc caaggccatg gggatcatga actccttcgt
     5161 caacgacatc ttcgagcgca tcgcgggcga ggcttctcgc ctggctcact acaataagcg
     5221 ctcgaccatc acctccaggg agattcagac ggctgtgcgc ctgctgctgc ctggggagct
     5281 ggctaagcat gctgtgtccg agggcactaa ggcagttacc aagtacacta gctctaagga
     5341 tccaccggtc gccaccatga gcaaaggaga agaacttttc actggagttg tcccaattct
     5401 tgttgaatta gatggtgatg ttaatgggca caaattttct gtccgtggag agggtgaagg
     5461 tgatgctaca aacggaaaac tcacccttaa atttatttgc actactggaa aactacctgt
     5521 tccgtggcca acacttgtca ctactctgac ctatggtgtt caatgctttt cccgttatcc
     5581 ggatcacatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     5641 acgcactata tctttcaaag atgacgggac ctacaagacg cgtgctgaag tcaagtttga
     5701 aggtgatacc cttgttaatc gtatcgagtt aaagggtatt gattttaaag aagatggaaa
     5761 cattcttgga cacaaactcg agtacaactt taactcacac aatgtataca tcacggcaga
     5821 caaacaaaag aatggaatca aagctaactt caaaattcgc cacaacgttg aagatggttc
     5881 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     5941 accagacaac cattacctgt cgacacaatc tgtcctttcg aaagatccca acgaaaagcg
     6001 tgaccacatg gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga
     6061 gctctacaaa tgattaatta aaacctctgg attacaaaat ttgtgaaaga ttgactggta
     6121 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6181 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6241 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6301 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6361 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6421 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6481 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6541 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6601 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6661 ccccgcagga tccactagag cggccgccta gagctcgctg atcagcctcg actgtgcctt
     6721 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6781 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6841 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6901 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6961 ggggctcgac tagagcttgc ggaaccctta agcttgtcat tcgaatttag acacccacat
     7021 ggtggctcat gaccatctat ctataaatgg atctgatttt catgtctggc agtgtacaga
     7081 agctaactga agaaaggtgg aagacccaca agagttcaag gtacccaatt cgccctatag
     7141 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7201 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7261 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     7321 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     7381 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     7441 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     7501 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     7561 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     7621 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     7681 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7741 gccgcgctta atgcgccgct acagggcgcg tcag
//
