LOCUS       CDS_nptII               2856 bp ds-DNA     linear       29-OCT-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-B06" "Type.plasmid:L0"          
            "Description" "Use:Confers plant resistance to kanamycin" "Plasmid
            size, bp:2856" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1371/journal.pone.0138876"
            "Registry.ID.JHLab:HMO_p0_o055" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    complement(1047..1052)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Replication_ori complement(1311..1899)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    244..1041
                     /label="nptII / KanR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      complement(2082..2176)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    934..940
                     /label="Restriction site removed"
                     /ApEinfo_revcolor=#f96381
                     /ApEinfo_fwdcolor=#f96381
     misc_feature    1042..1045
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2197..2856)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    724..730
                     /label="Restriction site removed"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cAaatgggga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
      301 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgtttcgg
      361 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
      421 gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
      481 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
      541 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
      601 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
      661 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
      721 gacgaggagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
      781 cccgacggcg aggatctcgt cgtgacacat ggcgatgcct gcttgccgaa tatcatggtg
      841 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
      901 caggacatag cgttggctac ccgtgatatt gctgaggagc ttggcggcga atgggctgac
      961 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     1021 cttcttgacg agttcttctg agcttCgaga ccCTGCAGTC CGGCAAAAAA GGGCAAGGTG
     1081 TCACCACCCT GCCCTTTTTC TTTAAAACCG AAAAGATTAC TTCGCGTTAT GCAGGCTTCC
     1141 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
     1201 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
     1261 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCACAGG
     1321 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     1381 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     1441 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     1501 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     1561 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1621 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     1681 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     1741 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     1801 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     1861 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     1921 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     1981 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     2041 AGTATATATG AGTAAACTTG GTCTGACAGC TCGAGGCTTG GATTCTCACC AATAAAAAAC
     2101 GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA
     2161 TCTATCAACA GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC
     2221 GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG
     2281 ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT
     2341 GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA
     2401 ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA
     2461 GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA
     2521 ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT
     2581 GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA
     2641 TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG
     2701 CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA
     2761 GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT
     2821 ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCAT
//