LOCUS       CDS_mALS                4095 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-B07" "Type.plasmid:L0"          
            "Description" "Use:Confers plant resistance to chlorsulfuron"
            "Plasmid size, bp:4095" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1584/jpestics.G07-29
            https\://doi.org/10.1371/journal.pone.0138876"
            "Registry.ID.JHLab:HMO_p0_o682" "Name.JHLab"
FEATURES             Location/Qualifiers
     CDS             complement(3436..4095)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Replication_ori complement(2550..3138)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2286..2291)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    244..2280
                     /label="mALS / CsR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2281..2284
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(3321..3415)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGAatggcgt caattagtgg atgcgcagct gtgcctttga gcatagcgag cctggagaag
      301 gtgcagccat cggcaaggtc ttgttgcaag ggagctaaag agctaaaggt gaggagtggt
      361 ttcttctcgg gagccgctct gaggggacat gcatacaaca cctctcggaa gactgagcgt
      421 tcactggaag tgaaagccca agctgttagc gagaagatcc agtctgcagt cgatgtgagt
      481 ggaccacggc ttagcagcgg gaagtcactc gttgcaccaa ccccgtccga ctttcagtct
      541 cgctacaagg atgacgagcc ccgcaaagga gccgacattt tggtggaggc tcttgagcga
      601 gaagggatta gaacggtgtt cgcttatccc ggcggagcgt ctatggagat tcaccaggct
      661 cttacacgtt ccagcagcat ccgcaacatt ctctgtcgcc acgagcaggg ggagattttc
      721 gctgctgaag gttatgctaa atcgtctgga cgagtagggg tttgtatagc cacatccggt
      781 cctggagcga ccaatcttgt taccgggctc gctgatgccc tcttggacag tgtgccacta
      841 gtggccatta caggtcaagt atccagtagg atgattggta ccgatgcttt tcaagaaact
      901 ccaattgtgg aggttacgag atcgatcacc aagcacaatt accttgttat gaacattgat
      961 gacatccctc gcgtaatcag ggaggctttc tttttggcag cttcaggtag gccgggccca
     1021 gtcttggtag atattcccaa ggacgtgcaa cagcaaatgg ctattccaga ttggtcGcag
     1081 ccaatgcaac tgaagggcta cttacaacga ctgcctcggc caccggaacc aacgttaatc
     1141 gagcagatta taagactcat ttctagttct aagaagcctg tagtgtattc tggaggaggt
     1201 tgcaatcacg ccagcgaaga gttgagggaa tttgtcgagc ttaccggaat tcctatcaca
     1261 tcgactttaa tgggtttggg agcgtatccc gagtctgatc cattaagttt gaaaatgctt
     1321 ggaatgcatg gaactgttta cgccaactac gccgtggacc aagcagatct gttgttggca
     1381 tttggagtgc gtttcgacga tcgagtgact ggtaaactgg agacttttgc tagcaggtcc
     1441 agcatcgttc atatcgatat tgaccctgct gaaattggaa agaacaaaca gccccatgtg
     1501 tccatgtgtg ctgatgtgaa acctgcgcta agtgaaatca atgccctctt gaagagtcaa
     1561 aagaggactt tcgacttctc caactgggtg gcagaactag aagaacaaaa gcagaaatgg
     1621 ccaatgaagt ttcccgtatt cgaggatgcc attgctcctc aatctgccat acagacgctg
     1681 tacgaactca ctggtggaaa tgccatcata tctactggtg ttggccaaca tcaaatgtgg
     1741 gcagcccagt ggttcaagta caacgaaccg cggcgttggt tgacatctgg aggtcttgga
     1801 gccatgggat tcggacttcc ttctgctttg ggggctgccg caactcaacc agatattcct
     1861 gtgatcgaca tcgatggcga tggtagcttt gtgatgaacg tacaggagtt ggctacaatt
     1921 tttgtggaga agttacccgt gaagatcatg atcttaaaca accaacatct aggaatggtc
     1981 gtacaattgg aggatagatt ctacaaggcc aacagagcac atacttactt aggtgatcca
     2041 gatcacgagg agcagatcta ccccgacttc tgtacagttg cagacggttt caaggtgcca
     2101 aatgcgcgtg tggtgaagaa ggatgaacta agagcagcaa ttcaaactat gctagattct
     2161 cccggtcctt atttactgga tgtgatagtt ccccaccagg agcacgttct gcctatgatt
     2221 cctgggggag caagttttaa ggagattatc acagagggtg atggaaggac tatatactaa
     2281 GCTTTgagac cCTGCAGTCC GGCAAAAAAG GGCAAGGTGT CACCACCCTG CCCTTTTTCT
     2341 TTAAAACCGA AAAGATTACT TCGCGTTATG CAGGCTTCCT CGCTCACTGA CTCGCTGCGC
     2401 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
     2461 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
     2521 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCACAGGC TCCGCCCCCC TGACGAGCAT
     2581 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     2641 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     2701 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     2761 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     2821 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     2881 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     2941 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     3001 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     3061 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     3121 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     3181 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     3241 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3301 TCTGACAGCT CGAGGCTTGG ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT
     3361 CTGAACAAAT CCAGATGGAG TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC
     3421 GAGCTCGATA TCAAATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT
     3481 AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG
     3541 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA
     3601 GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA
     3661 GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA
     3721 CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA
     3781 GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC
     3841 CCATATCACC AGCTCACCGT CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG
     3901 GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT
     3961 TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG
     4021 AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT
     4081 GATTTTTTTC TCCAT
//