LOCUS       CDS_hptII               3084 bp ds-DNA     circular     04-NOV-2019
DEFINITION  .
KEYWORDS    "Addgene" "OP.kit.position:Plate01-B08" "Type.plasmid:L0"          
            "Description" "Use:Confers plant resistance to hygromycin" "Plasmid
            size, bp:3084" "Bacteria resistance:Cm" "Plant resistance"
            "Phytozome.ID.v3" "Reference:Sauret-Gueto S, Frangedakis E, et al
            (in preparation)" "Primary
            publication:https\://doi.org/10.1093/pcp/pcn085"
            "Registry.ID.JHLab:HMO_p0_o687" "Name.JHLab"
FEATURES             Location/Qualifiers
     misc_feature    236..241
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(1539..2127)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1270..1273
                     /label="L0 fusion site GCTT"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(2425..3084)
                     /label="cat / CmR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             244..1269
                     /label="hptII / HygR (seq a bit diff from MoClo, but same AA, except Q>K)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1275..1280)
                     /label="BsaI recog site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      complement(2310..2404)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    243..246
                     /label="L0 fusion site AATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT
       61 TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA GTGCCACCTG
      121 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC
      181 AGAATTTCAG ATAAAAAAAA TCCTTAGCTT TCGCTAAGGA TGATTTCTGG AATTCggtct
      241 cGAatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
      301 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      361 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      421 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
      481 attggggagt ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg
      541 ttgcaagacc tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg
      601 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
      661 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
      721 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
      781 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
      841 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
      901 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
      961 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     1021 ccacgactcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     1081 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     1141 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     1201 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     1261 aagaaatagG CTTTgagacc CTGCAGTCCG GCAAAAAAGG GCAAGGTGTC ACCACCCTGC
     1321 CCTTTTTCTT TAAAACCGAA AAGATTACTT CGCGTTATGC AGGCTTCCTC GCTCACTGAC
     1381 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA
     1441 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     1501 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCACAGGCT CCGCCCCCCT
     1561 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     1621 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     1681 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     1741 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     1801 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     1861 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     1921 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA
     1981 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     2041 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     2101 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2161 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     2221 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2281 TAAACTTGGT CTGACAGCTC GAGGCTTGGA TTCTCACCAA TAAAAAACGC CCGGCGGCAA
     2341 CCGAGCGTTC TGAACAAATC CAGATGGAGT TCTGAGGTCA TTACTGGATC TATCAACAGG
     2401 AGTCCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG
     2461 TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT
     2521 CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG
     2581 GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG
     2641 ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC
     2701 ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA
     2761 TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG
     2821 AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC
     2881 ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT
     2941 TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC
     3001 AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT
     3061 ATATCCAGTG ATTTTTTTCT CCAT
//